Rabbit PSMA6 Polyclonal Antibody | anti-PSMA6 antibody

PSMA6 Polyclonal Antibody

Cat. Num. AAA127790

• Gene Names: PSMA6; IOTA; p27K; PROS27
• Reactivity: Human, Mouse, Monkey
• Applications: Western Blot, Immunohistochemistry, Immunofluorescence
• Purity: Affinity Purification
• Synonyms: PSMA6; Polyclonal Antibody; PSMA6 Polyclonal Antibody; IOTA; MGC22756; MGC2333; MGC23846; PROS27; p27K; anti-PSMA6 antibody

• Host: Rabbit
• Reactivity: Human, Mouse, Monkey
• Clonality: Polyclonal
• Isotype: IgG
• Purity/Purification: Affinity Purification
• Concentration: 1mg/ml (varies by lot)
• Sequence Length: 246

• Applicable Applications for anti-PSMA6 antibody: Western Blot (WB), Immunohistochemistry (IHC), Immunofluorescence (IF)
• Application Notes: WB: 1:500 - 1:2000; IHC: 1:50 - 1:200; IF: 1:50 - 1:200
• Species: Human
• Immunogen: Recombinant Protein
• Immunogen: Recombinant protein of human PSMA6
• Calculated Molecular Weight: 27kDa
• Preparation and Storage: Store at -20 degree C (regular) or -80 degree C (long term). Avoid freeze / thaw cycles.; Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3.

Western Blot (WB)

(Western blot analysis of extracts of various cell lines, using PSMA6 antibody at 1:1000 dilution.Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (MBS128200) at 1:10000 dilution.Lysates/proteins: 25ug per lane.Blocking buffer: 3% nonfat dry milk in TBST.)

Immunofluorescence (IF)

(Immunofluorescence analysis of U2OS cells using PSMA6 antibody. Blue: DAPI for nuclear staining.)

Related Product Information for anti-PSMA6 antibody

The 20S proteasome is the major proteolytic enzyme complex involved in intracellular protein degradation. It consists of four stacked rings, each with seven distinct subunits. The two outer layers are identical rings composed of alpha subunits (called PSMAs), and the two inner layers are identical rings composed of beta subunits. While the catalytic sites are located on the beta rings (1-3), the alpha subunits are important for assembly and as binding sites for regulatory proteins (4). Seven different alpha and ten different beta proteasome genes have been identified in mammals (5). PA700, PA28, and PA200 are three major protein complexes that function as activators of the 20S proteasome. PA700 binds polyubiquitin with high affinity and associates with the 20S proteasome to form the 26S proteasome, which preferentially degrades poly-ubiquitinated proteins (1-3). The proteasome has a broad substrate spectrum that includes cell cycle regulators, signaling molecules, tumor suppressors, and transcription factors. By controlling the degradation of these intracellular proteins, the proteasome functions in cell cycle regulation, cancer development, immune responses, protein folding, and disease progression (6-9).

Product Categories/Family for anti-PSMA6 antibody

Polyclonal

NCBI and Uniprot Product Information

• NCBI GI #: 46397659
• NCBI GeneID: 5687
• UniProt Accession #: P60900; P34062; Q6IB60; B2R7J9; B4DQR4; B4DXJ9
• Molecular Weight: 246
• NCBI Official Full Name: Proteasome subunit alpha type-6
• NCBI Official Synonym Full Names: proteasome (prosome, macropain) subunit, alpha type, 6
• NCBI Official Symbol: PSMA6
• NCBI Official Synonym Symbols: IOTA; p27K; PROS27
• NCBI Protein Information: proteasome subunit alpha type-6; PROS-27; macropain iota chain; prosomal P27K protein; proteasome iota chain; macropain subunit iota; 27 kDa prosomal protein; proteasome subunit iota; multicatalytic endopeptidase complex iota chain
• UniProt Protein Name: Proteasome subunit alpha type-6
• Protein Family: Proteasome
• UniProt Gene Name: PSMA6
• UniProt Synonym Gene Names: PROS27; PROS-27; p27K
• UniProt Entry Name: PSA6_HUMAN

NCBI Description

The proteasome is a multicatalytic proteinase complex with a highly ordered ring-shaped 20S core structure. The core structure is composed of 4 rings of 28 non-identical subunits; 2 rings are composed of 7 alpha subunits and 2 rings are composed of 7 beta subunits. Proteasomes are distributed throughout eukaryotic cells at a high concentration and cleave peptides in an ATP/ubiquitin-dependent process in a non-lysosomal pathway. An essential function of a modified proteasome, the immunoproteasome, is the processing of class I MHC peptides. This gene encodes a member of the peptidase T1A family, that is a 20S core alpha subunit. Multiple transcript variants encoding several different isoforms have been found for this gene. A pseudogene has been identified on the Y chromosome. [provided by RefSeq, Aug 2013]

Uniprot Description

PSMA6: The proteasome is a multicatalytic proteinase complex which is characterized by its ability to cleave peptides with Arg, Phe, Tyr, Leu, and Glu adjacent to the leaving group at neutral or slightly basic pH. The proteasome has an ATP-dependent proteolytic activity. The 26S proteasome consists of a 20S proteasome core and two 19S regulatory subunits. The 20S proteasome core is composed of 28 subunits that are arranged in four stacked rings, resulting in a barrel-shaped structure. The two end rings are each formed by seven alpha subunits, and the two central rings are each formed by seven beta subunits. The catalytic chamber with the active sites is on the inside of the barrel. Belongs to the peptidase T1A family.

Protein type: Proteasome complex; EC 3.4.25.1; Protease

Chromosomal Location of Human Ortholog: 14q13

Cellular Component: proteasome complex; nucleoplasm; polysome; proteasome core complex; sarcomere; nuclear matrix; myofibril; cytoplasm; nucleus; cytosol

Molecular Function: threonine endopeptidase activity; NF-kappaB binding; protein binding; RNA binding; endopeptidase activity

Biological Process: positive regulation of ubiquitin-protein ligase activity during mitotic cell cycle; negative regulation of ubiquitin-protein ligase activity during mitotic cell cycle; protein polyubiquitination; viral reproduction; apoptosis; antigen processing and presentation of exogenous peptide antigen via MHC class I, TAP-dependent; DNA damage response, signal transduction by p53 class mediator resulting in cell cycle arrest; activation of NF-kappaB transcription factor; regulation of apoptosis; antigen processing and presentation of peptide antigen via MHC class I; proteolysis involved in cellular protein catabolic process; regulation of ubiquitin-protein ligase activity during mitotic cell cycle; anaphase-promoting complex-dependent proteasomal ubiquitin-dependent protein catabolic process; regulation of inflammatory response; antigen processing and presentation of exogenous peptide antigen via MHC class I; gene expression; mitotic cell cycle; regulation of amino acid metabolic process; G1/S transition of mitotic cell cycle; negative regulation of apoptosis

Disease: Myocardial Infarction, Susceptibility To