Rabbit anti-Human PRMT7 Polyclonal Antibody | anti-PRMT7 antibody

Anti-PRMT7 Antibody, Rabbit Polyclonal

Cat. Num. AAA8100922

• Reactivity: Human
• Applications: Immunohistochemistry, Immunocytochemistry, Immunofluorescence
• Purity: Protein A & Antigen Affinity
• Synonyms: PRMT7; Polyclonal Antibody; Anti-PRMT7 Antibody; Rabbit Polyclonal; PRMT7 Antibody; Rabbit PAb; Antigen Affinity Purified; anti-PRMT7 antibody

• Host: Rabbit
• Reactivity: Human
• Clonality: Polyclonal
• Isotype: Rabbit IgG
• Specificity: Human PRMT7
• Purity/Purification: Protein A & Antigen Affinity
• Form/Format: Liquid; 0.2um filtered solution in PBS

• Applicable Applications for anti-PRMT7 antibody: Immunohistochemistry-Paraffin (IHC-P), Immunocytochemistry (ICC), Immunofluorescence (IF)
• Application Notes: IHC-P: 1:1000-1:4000; ICC/IF: 1:300-1:10,000
• Immunogen: A synthetic peptide corresponding to the center region of the Human PRMT7
• Conjugation: Unconjugated
• Preparation: Produced in rabbits immunized with a synthetic peptide corresponding to the center region of the Human PRMT7, and purified by antigen affinity chromatography.
• Preparation and Storage: This antibody can be stored at 2-8 degree C for one month without detectable loss of activity. Antibody products are stable for twelve months from date of receipt when stored at -20 degree C to -80 degree C. Preservative-Free. Avoid repeated freeze-thaw cycles.

Immunohistochemistry (IHC)

(Immunochemical staining of human PRMT7 in human breast carcinoma with rabbit polyclonal antibody (1:2000, formalin-fixed paraffin embedded sections).)

Immunofluorescence (IF)

(Immunofluorescence staining of PRMT7 in HeLa cells. Cells were fixed with 4% PFA, permeabilzed with 0.3% Triton X-100 in PBS,blocked with 10% serum, and incubated with rabbit anti-human PRMT7 polyclonal antibody (1:1000) at 4 degree C overnight. Then cells were stained with the Alexa Fluor488-conjugated Goat Anti-rabbit IgG secondary antibody (green).Positive staining was localized to cytoplasm and nucleus.)

Related Product Information for anti-PRMT7 antibody

PRMT7 belongs to the protein arginine methyl-transferases family. Protein arginine methyltransferase 7 (PRMT7) is a member of a family of enzymes that catalyze the transfer of methyl groups from S-adenosyl-l-methionine to nitrogen atoms on arginine residues. Arginine methylation is involved in multiple biological processes, such as signal transduction, mRNA splicing, transcriptional control, DNA repair, and protein translocation. PRMT7 encodes for an arginine methyltransferase that methylates arginine residues on various protein substrates and has been shown to play a role in various developmental processes. Mutations in PRMT7 have been recently shown to be implicated in a phenotype with intellectual disability, short stature and brachydactyly, and considered to be a phenocopy of pseudohypoparathyroidism.

NCBI and Uniprot Product Information

• NCBI GI #: 9506615
• NCBI GeneID: 54496
• NCBI Accession #: NP_061896.1
• NCBI GenBank Nucleotide #: NM_019023.2
• UniProt Accession #: Q9NVM4; Q96PV5; Q9H9L0; B3KPR0; B3KUG9; B4E379
• Molecular Weight: 63,806 Da
• NCBI Official Full Name: protein arginine N-methyltransferase 7 isoform 1
• NCBI Official Synonym Full Names: protein arginine methyltransferase 7
• NCBI Official Symbol: PRMT7
• NCBI Protein Information: protein arginine N-methyltransferase 7; histone-arginine N-methyltransferase PRMT7; myelin basic protein-arginine N-methyltransferase
• UniProt Protein Name: Protein arginine N-methyltransferase 7
• UniProt Gene Name: PRMT7
• UniProt Synonym Gene Names: KIAA1933
• UniProt Entry Name: ANM7_HUMAN

NCBI Description

Arginine methylation is an apparently irreversible protein modification catalyzed by arginine methyltransferases, such as PMT7, using S-adenosylmethionine (AdoMet) as the methyl donor. Arginine methylation is implicated in signal transduction, RNA transport, and RNA splicing (Miranda et al., 2004 [PubMed 15044439]).[supplied by OMIM, Mar 2008]

Uniprot Description

PRMT7: Arginine methyltransferase that can both catalyze the formation of omega-N monomethylarginine (MMA) and symmetrical dimethylarginine (sDMA), with a preference for the formation of MMA. Specifically mediates the symmetrical dimethylation of arginine residues in the small nuclear ribonucleoproteins Sm D1 (SNRPD1) and Sm D3 (SNRPD3); such methylation being required for the assembly and biogenesis of snRNP core particles. Specifically mediates the symmetric dimethylation of histone H4 'Arg-3' to form H4R3me2s. Plays a role in gene imprinting by being recruited by CTCFL at the H19 imprinted control region (ICR) and methylating histone H4 to form H4R3me2s, possibly leading to recruit DNA methyltransferases at these sites. May also play a role in embryonic stem cell (ESC) pluripotency. Also able to mediate the arginine methylation of histone H2A and myelin basic protein (MBP) in vitro; the relevance of such results is however unclear in vivo. Belongs to the protein arginine N-methyltransferase family. PRMT7 subfamily. 4 isoforms of the human protein are produced by alternative splicing.

Protein type: EC 2.1.1.126; EC 2.1.1.125; Methyltransferase, protein arginine; Methyltransferase; RNA processing

Chromosomal Location of Human Ortholog: 16q22.1

Cellular Component: nucleoplasm; plasma membrane; nucleolus; cytosol; nucleus

Molecular Function: ribonucleoprotein binding; protein-arginine omega-N asymmetric methyltransferase activity; protein-arginine omega-N monomethyltransferase activity; [myelin basic protein]-arginine N-methyltransferase activity; histone binding; protein-arginine omega-N symmetric methyltransferase activity; S-adenosylmethionine-dependent methyltransferase activity; histone-arginine N-methyltransferase activity

Biological Process: histone methylation; peptidyl-arginine methylation, to symmetrical-dimethyl arginine; establishment and/or maintenance of chromatin architecture; spliceosomal snRNP biogenesis; peptidyl-arginine methylation; genetic imprinting; regulation of transcription, DNA-dependent; transcription, DNA-dependent; DNA methylation during gametogenesis; regulation of protein binding; peptidyl-arginine methylation, to asymmetrical-dimethyl arginine; cell differentiation