Western Blot (WB)
(Western blot analysis of PRR5L in 3T3 cell lysate with PRR5L antibody at 1 ug/mL in (A) the absence and (B) the presence of blocking peptide)
Rabbit anti-Human, Mouse PRR5L Polyclonal Antibody | anti-PRR5L antibody
PRR5L Antibody
Gene Names
PRR5L; PROTOR2
Reactivity
Human, Mouse
Applications
ELISA, Western Blot
Purity
Affinity chromatography purified via peptide column
Synonyms
PRR5L; Polyclonal Antibody; PRR5L Antibody; Proline-rich protein 5-like; Protor-2; PROTOR2; anti-PRR5L antibody
Host
Rabbit
Reactivity
Human, Mouse
Clonality
Polyclonal
Specificity
At least three isoforms of PRR5L are known to exist; this antibody will recognize only isoforms a and b. PRR5L antibody will not cross-react with PPR5.
Purity/Purification
Affinity chromatography purified via peptide column
Form/Format
Supplied in PBS containing 0.02% sodium azide.
Sequence Length
368
Applicable Applications for anti-PRR5L antibody
ELISA (EIA), Western Blot (WB)
Immunogen Type
Peptide
Immunogen Description
Raised against a 17 amino acid peptide near the center of human PRR5L.
Target Name
PRR5L
Preparation and Storage
Can be stored at -20 degree C, stable for one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures.
Western Blot (WB)
(Western blot analysis of PRR5L in 3T3 cell lysate with PRR5L antibody at 1 ug/mL in (A) the absence and (B) the presence of blocking peptide)
Western Blot (WB)
(Western blot analysis of PRR5L in 3T3 cell lysate with PRR5L antibody at 1 ug/mL in (A) the absence and (B) the presence of blocking peptide)
Related Product Information for anti-PRR5L antibody
Proline-rich protein 5-like (PRR5L), also known as Protor-2, is structurally similar to PRR5 and also binds to the mTOR complex 2 (mTORC2). PRR5L is not required for mTORC2 integrity or kinase activity, but dissociates from mTORC2 upon knockdown of tuberous sclerosis complex 1 (TSC1) and TSC2. Knockdown of PRR5L reduced apoptosis in mTORC2 deficient cells as well as cells where mTOR was active, suggesting that mTORC2-dissociated PRR5L may promote apoptosis, and thus play a role in the balance between cell growth and cell death.
Product Categories/Family for anti-PRR5L antibody
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
Molecular Weight
23,005 Da
NCBI Official Full Name
proline-rich protein 5-like isoform a
NCBI Official Synonym Full Names
proline rich 5 like
NCBI Official Symbol
PRR5L
NCBI Official Synonym Symbols
PROTOR2
NCBI Protein Information
proline-rich protein 5-like
UniProt Protein Name
Proline-rich protein 5-like
Protein Family
UniProt Gene Name
PRR5L
UniProt Synonym Gene Names
PROTOR2; Protor-2
UniProt Entry Name
PRR5L_HUMAN
Uniprot Description
PROTOR2: May be part of the TORC2 complex which plays a critical role in AKT1 'Ser-473' phosphorylation, and may modulate the phosphorylation of PKCA and regulate actin cytoskeleton organization. Belongs to the PROTOR family. May replace PROTOR1 in the target of rapamycin 2 complex (TORC2) comprised of FRAP1, LST8, PROTOR1, RICTOR and MAPKAP1. 3 isoforms of the human protein are produced by alternative splicing.
Chromosomal Location of Human Ortholog: 11p13-p12
Cellular Component: mitochondrion; TORC2 complex
Molecular Function: ubiquitin protein ligase binding
Biological Process: positive regulation of phosphoinositide 3-kinase cascade; negative regulation of protein amino acid phosphorylation; positive regulation of protein amino acid phosphorylation; negative regulation of signal transduction
Research Articles on PRR5L
Similar Products
Product Notes
The PRR5L prr5l (Catalog #AAA9413235) is an Antibody produced from Rabbit and is intended for research purposes only. The product is available for immediate purchase. The PRR5L Antibody reacts with Human, Mouse and may cross-react with other species as described in the data sheet. AAA Biotech's PRR5L can be used in a range of immunoassay formats including, but not limited to, ELISA (EIA), Western Blot (WB). Researchers should empirically determine the suitability of the PRR5L prr5l for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "PRR5L, Polyclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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