Rabbit PSMA6 Antibody | anti-PSMA6 antibody

PSMA6 Antibody

Cat. Num. AAA8501704

• Gene Names: PSMA6; IOTA; p27K; PROS27
• Reactivity: Human, Mouse, Rat
• Applications: Western Blot, Immunohistochemistry
• Purity: Affinity purification
• Synonyms: PSMA6; PSMA6 Antibody; IOTA; MGC22756; MGC2333; MGC23846; PROS27; p27K; anti-PSMA6 antibody

• Host: Rabbit
• Reactivity: Human, Mouse, Rat
• Isotype: IgG
• Purity/Purification: Affinity purification
• Sequence Length: 167

• Applicable Applications for anti-PSMA6 antibody: Western Blot (WB), Immunohistochemistry (IHC)
• Application Notes: WB: 1:500-1:2000; IHC: 1:50-1:200
• Immunogen: Recombinant protein of human PSMA6
• Storage Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
• Preparation and Storage: Store at-20 degree C or-80 degree C. Avoid freeze/thaw cycles.

Western Blot (WB)

(Western blot analysis of extracts of various celllines, using PSMA6 antibody.)

Related Product Information for anti-PSMA6 antibody

The 20S proteasome is the major proteolytic enzyme complex involved in intracellular protein degradation. It consists of four stacked rings, each with seven distinct subunits. The two outer layers are identical rings composed of alpha subunits (called PSMAs), and the two inner layers are identical rings composed of beta subunits. While the catalytic sites are located on the beta rings (1-3), the alpha subunits are important for assembly and as binding sites for regulatory proteins (4). Seven different alpha and ten different beta proteasome genes have been identified in mammals (5). PA700, PA28, and PA200 are three major protein complexes that function as activators of the 20S proteasome. PA700 binds polyubiquitin with high affinity and associates with the 20S proteasome to form the 26S proteasome, which preferentially degrades poly-ubiquitinated proteins (1-3). The proteasome has a broad substrate spectrum that includes cell cycle regulators, signaling molecules, tumor suppressors, and transcription factors. By controlling the degradation of these intracellular proteins, the proteasome functions in cell cycle regulation, cancer development, immune responses, protein folding, and disease progression (6-9).

Product Categories/Family for anti-PSMA6 antibody

Autophagy antibody; Cancer; Cardiovascular; Cell Biology; Epigenetics & Nuclear Signaling; Developmental Biologys; Immunology; Drug Discovery Products; Metabolism; Neuroscience; Signal Transduction; Stem Cells

References

1. Dahlmann, B. (2005) Essays Biochem. 41, 31-48. 2. Pickart, C.M. and Cohen, R.E. (2004) Nat. Rev. Mol. Cell Biol. 5, 177-187. 3. Nandi, D. et al. (2006) J. Biosci. 31, 137-155. 4. Lupas, A. et al. (1993) Enzyme Protein 47, 252-273. 5. Monaco, J.J. and Nandi, D. (1995) Annu. Rev. Genet. 29, 729-754. 6. Murray, A.W. (2004) Cell 116, 221-234. 7. Ciechanover, A. (2006) Proc. Am. Thorac. Soc. 3, 21-31. 8. Wang, J. and Maldonado, M.A. (2006) Cell. Mol. Immunol. 3, 255-261. 9. Rubinsztein, D.C. (2006) Nature 443, 780-786.

NCBI and Uniprot Product Information

• NCBI GI #: 532691770
• NCBI GeneID: 5687
• NCBI Accession #: NP_001269161.1
• NCBI GenBank Nucleotide #: NM_001282232.1
• UniProt Accession #: P60900; P34062; Q6IB60; B2R7J9; B4DQR4; B4DXJ9
• Molecular Weight: 27kDa
• NCBI Official Full Name: proteasome subunit alpha type-6 isoform b
• NCBI Official Synonym Full Names: proteasome subunit alpha 6
• NCBI Official Symbol: PSMA6
• NCBI Official Synonym Symbols: IOTA; p27K; PROS27
• NCBI Protein Information: proteasome subunit alpha type-6
• UniProt Protein Name: Proteasome subunit alpha type-6
• Protein Family: Proteasome
• UniProt Gene Name: PSMA6
• UniProt Synonym Gene Names: PROS27; PROS-27; p27K

NCBI Description

The proteasome is a multicatalytic proteinase complex with a highly ordered ring-shaped 20S core structure. The core structure is composed of 4 rings of 28 non-identical subunits; 2 rings are composed of 7 alpha subunits and 2 rings are composed of 7 beta subunits. Proteasomes are distributed throughout eukaryotic cells at a high concentration and cleave peptides in an ATP/ubiquitin-dependent process in a non-lysosomal pathway. An essential function of a modified proteasome, the immunoproteasome, is the processing of class I MHC peptides. This gene encodes a member of the peptidase T1A family, that is a 20S core alpha subunit. Multiple transcript variants encoding several different isoforms have been found for this gene. A pseudogene has been identified on the Y chromosome. [provided by RefSeq, Aug 2013]

Uniprot Description

Component of the 20S core proteasome complex involved in the proteolytic degradation of most intracellular proteins. This complex plays numerous essential roles within the cell by associating with different regulatory particles. Associated with two 19S regulatory particles, forms the 26S proteasome and thus participates in the ATP-dependent degradation of ubiquitinated proteins. The 26S proteasome plays a key role in the maintenance of protein homeostasis by removing misfolded or damaged proteins that could impair cellular functions, and by removing proteins whose functions are no longer required. Associated with the PA200 or PA28, the 20S proteasome mediates ubiquitin-independent protein degradation. This type of proteolysis is required in several pathways including spermatogenesis (20S-PA200 complex) or generation of a subset of MHC class I-presented antigenic peptides (20S-PA28 complex).