Testing Data
(Formalin-fixed, paraffin-embedded breast carcinoma stained with Phosphotyrosine Ab (PY20). Note cell surface and cytoplasmic staining.)
Mouse anti-All species Phosphotyrosine Monoclonal Antibody
Phosphotyrosine
Reactivity
All species
Applications
ELISA, Flow Cytometry, Functional Assay, Immunofluorescence, Western Blot, Immunoprecipitation, Immunohistochemistry
Synonyms
Phosphotyrosine; Monoclonal Antibody; P-Tyr; anti-Phosphotyrosine antibody
Host
Mouse
Reactivity
All species
Clonality
Monoclonal
Isotype
IgG2b
Clone Number
PY20
Specificity
Protein phosphorylation is a fundamental event in the regulation of a large number of intracellular processes. Phosphorylation of specific tyrosine residues is the result of activation or stimulation of their respective protein tyrosine kinases. The phosphorylated proteins can be auto-phosphorylated kinases or certain cellular protein substrates. Tyrosine-phosphorylated proteins are involved in signal transduction and in the regulation of cell proliferation. Antibody to phosphotyrosine provides an excellent tool for the detection, characterization, and purification of phosphotyrosine containing proteins. This MAb shows no cross-reaction with other phosphoamino acids and is superb for multiple applications including staining of formalin/paraffin tissues.
Form/Format
200ug/ml of Ab purified from Bioreactor Concentrate by Protein A/G. Prepared in 10mM PBS with 0.05% BSA & 0.05% azide. Also available WITHOUT BSA & azide at 1.0mg/ml.
Applicable Applications for anti-Phosphotyrosine antibody
ELISA (EIA), Flow Cytometry (FC/FACS), Immunofluorescence (IF), Western Blot (WB), Immunoprecipitation (IP), Immunohistology (IH)
Application Notes
ELISA (For coating, order Ab without BSA); Flow Cytometry (0.5-1ug/million cells); Immunofluorescence (1-2ug/ml); Western Blotting (0.5-1ug/ml); Immunoprecipitation (1-2ug/500ug protein lysate); Immunohistology (Frozen & Formalin-fixed) (1-2ug/ml for 30 minutes at RT) (No pretreatment is required for staining of formalin-fixed, paraffin-embedded tissues) Optimal dilution for a specific application should be determined.
Localization
Depends upon the phosphorylated target
Positive Control
MCF-7, MDA-231, T47-D cells or breast carcinoma
Immunogen
Phosphotyrosine conjugated to KLH
Quantity and Buffer
0.5 ml at 200ug/ml with BSA and azide
Preparation and Storage
Store at 2 to 8 degree C. Antibody is stable for 24 months.
Testing Data
(Formalin-fixed, paraffin-embedded breast carcinoma stained with Phosphotyrosine Ab (PY20). Note cell surface and cytoplasmic staining.)
Testing Data
(Formalin-fixed, paraffin-embedded breast carcinoma stained with Phosphotyrosine Ab (PY20). Note cell surface and cytoplasmic staining.)
References
1. Glenny, Jr. JR, et. al. Journal of Immunological Methods, 1988, 109:277-85.
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Product Notes
The Phosphotyrosine (Catalog #AAA438085) is an Antibody produced from Mouse and is intended for research purposes only. The product is available for immediate purchase. The Phosphotyrosine reacts with All species and may cross-react with other species as described in the data sheet. AAA Biotech's Phosphotyrosine can be used in a range of immunoassay formats including, but not limited to, ELISA (EIA), Flow Cytometry (FC/FACS), Immunofluorescence (IF), Western Blot (WB), Immunoprecipitation (IP), Immunohistology (IH). ELISA (For coating, order Ab without BSA); Flow Cytometry (0.5-1ug/million cells); Immunofluorescence (1-2ug/ml); Western Blotting (0.5-1ug/ml); Immunoprecipitation (1-2ug/500ug protein lysate); Immunohistology (Frozen & Formalin-fixed) (1-2ug/ml for 30 minutes at RT) (No pretreatment is required for staining of formalin-fixed, paraffin-embedded tissues) Optimal dilution for a specific application should be determined. Researchers should empirically determine the suitability of the Phosphotyrosine for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "Phosphotyrosine, Monoclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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