Western Blot (WB)
(Western Blot: Sample: Recombinant protein.)
Rabbit anti-Rat Never In Mitosis Gene A Related Kinase 2 (NEK2) Polyclonal Antibody | anti-NEK2 antibody
Polyclonal Antibody to Never In Mitosis Gene A Related Kinase 2 (NEK2)
MGSSHHHHHH SSGLVPRGSH MASMTGGQQM GRGS-VLHR DLKPANVFLD SKHNVKLGDF GLARILNHDT SFAKTFVGTPYYMSPEQISR LSYNEKSDIW SLGCLLYELC ALMPPFTAFN QKELAGKIRE GRFRRIPYRY SDGLNDLITR MLNLKDYHRP SVEEILESPL IADLVAEEQR RNLERRGRRS GEPSKLQDSSPVLSELKLKE RQLQDRERAL RAREDSLEQK ERELCIRERL AEDKLARAES LLKNYSLLKEHRLLCLAGGS ELDLPSSALK KKVHFHGESK ENTARSENSE
Immunocytochemistry in formalin fixed cells: 1:100-500
Immunohistochemistry in formalin fixed frozen section: 1:100-500
Immunohistochemistry in paraffin section: 1:50-200
Enzyme-linked Immunosorbent Assay: 1:100-200
Western Blot (WB)
(Western Blot: Sample: Recombinant protein.)
Western Blot (WB)
(Western Blot: Sample: Recombinant protein.)
Immunohistochemistry (IHC)
(DABstainingonIHC-P.Samples:RatTissue))
Immunohistochemistry (IHC)
(DABstainingonIHC-P.Samples:RatTissue))
NCBI and Uniprot Product Information
NCBI Description
mouse homolog displays cell cycle associated changes in nuclear and cytoplasmic localization; may act to regulate mitosis [RGD, Feb 2006]
Uniprot Description
NEK2: a protein kinase of the NEK family. Accumulates throughout S phase and shows maximal levels in late G2. This expression pattern is highly reminiscent of that of A and B cyclins. Phosphorylation of Hec1 by Nek2 is essential for faithful chromosome segregation
Protein type: EC 2.7.11.1; Kinase, protein; NEK family; Nucleolus; Other group; Protein kinase, Other; Protein kinase, Ser/Thr (non-receptor)
Chromosomal Location of Human Ortholog: 13q27
Cellular Component: centrosome; condensed nuclear chromosome; kinetochore; midbody; nucleoplasm; protein complex; spindle pole
Molecular Function: protein kinase activity; protein phosphatase binding
Biological Process: blastocyst development; centrosome separation; chromosome segregation; mitotic sister chromatid segregation; mitotic spindle assembly; negative regulation of DNA binding; positive regulation of telomerase activity; positive regulation of telomere capping; positive regulation of telomere maintenance via telomerase; protein amino acid phosphorylation; protein autophosphorylation; regulation of attachment of spindle microtubules to kinetochore; regulation of mitotic centrosome separation
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Product Notes
The NEK2 nek2 (Catalog #AAA2001516) is an Antibody produced from Rabbit and is intended for research purposes only. The product is available for immediate purchase. The Polyclonal Antibody to Never In Mitosis Gene A Related Kinase 2 (NEK2) reacts with Rat and may cross-react with other species as described in the data sheet. AAA Biotech's Never In Mitosis Gene A Related Kinase 2 (NEK2) can be used in a range of immunoassay formats including, but not limited to, Immunocytochemistry (ICC), Immunohistochemistry (IHC) - Formalin/Paraffin, ELISA (EIA), Western Blot (WB). Western blotting: 1:100-400 Immunocytochemistry in formalin fixed cells: 1:100-500 Immunohistochemistry in formalin fixed frozen section: 1:100-500 Immunohistochemistry in paraffin section: 1:50-200 Enzyme-linked Immunosorbent Assay: 1:100-200. Researchers should empirically determine the suitability of the NEK2 nek2 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. The amino acid sequence is listed below: Antigen: The target protein is fused with two N-terminal Tags, His-tag and its sequence is listed below. MGSSHHHHHH SSGLVPRGSH MASMTGGQQM GRGS-VLHR DLKPANVFLD SKHNVKLGDF GLARILNHDT SFAKTFVGTP YYMSPEQISR LSYNEKSDIW SLGCLLYELC ALMPPFTAFN QKELAGKIRE GRFRRIPYRY SDGLNDLITR MLNLKDYHRP SVEEILESPL IADLVAEEQR RNLERRGRRS GEPSKLQDSS PVLSELKLKE RQLQDRERAL RAREDSLEQK ERELCIRERL AEDKLARAES LLKNYSLLKE HRLLCLAGGS ELDLPSSALK KKVHFHGESK ENTARSENSE. It is sometimes possible for the material contained within the vial of "Never In Mitosis Gene A Related Kinase 2 (NEK2), Polyclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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