Rabbit CSK Polyclonal Antibody | anti-CSK antibody

CSK Antibody (N-term)

Cat. Num. AAA9216480

• Reactivity: Human, Mouse, Rat
• Applications: Western Blot
• Synonyms: CSK; Polyclonal Antibody; CSK Antibody (N-term); Purified Rabbit Polyclonal Antibody (Pab); Tyrosine-protein kinase CSK; C-Src kinase; Protein-tyrosine kinase CYL; anti-CSK antibody

• Host: Rabbit
• Reactivity: Human, Mouse, Rat
• Clonality: Polyclonal
• Isotype: Ig
• Specificity: This CSK antibody is generated from a rabbit immunized with a KLH conjugated synthetic peptide between 17-51 amino acids from the N-terminal region of human CSK.
• Form/Format: Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein A column, followed by peptide affinity purification.
• Sequence Length: 450

• Applicable Applications for anti-CSK antibody: Western Blot (WB)
• Application Notes: WB ~~ 1:2000
• Antigen Source: Human
• Preparation and Storage: Maintain refrigerated at 2-8 degree C for up to 6 months. For long term storage store at -20 degree C in small aliquots to prevent freeze-thaw cycles.

Western Blot (WB)

(All lanes : Anti-CSK Antibody (N-term) at 1:2000 dilutionLane 1: C6 whole cell lysatesLane 2: Ramos whole cell lysatesLane 3: A549 whole cell lysatesLane 4: mouse spleen lysatesLysates/proteins at 20 ug per lane.SecondaryGoat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/10000 dilution.Predicted band size : 51 kDaBlocking/Dilution buffer: 5% NFDM/TBST.)

Related Product Information for anti-CSK antibody

Non-receptor tyrosine-protein kinase that plays an important role in the regulation of cell growth, differentiation, migration and immune response. Phosphorylates tyrosine residues located in the C-terminal tails of Src-family kinases (SFKs) including LCK, SRC, HCK, FYN, LYN or YES1. Upon tail phosphorylation, Src-family members engage in intramolecular interactions between the phosphotyrosine tail and the SH2 domain that result in an inactive conformation. To inhibit SFKs, CSK is recruited to the plasma membrane via binding to transmembrane proteins or adapter proteins located near the plasma membrane. Suppresses signaling by various surface receptors, including T- cell receptor (TCR) and B-cell receptor (BCR) by phosphorylating and maintaining inactive several positive effectors such as FYN or LCK.

Product Categories/Family for anti-CSK antibody

Crown Antibodies; Signal Transduction

References

Partanen J.,et al.Oncogene 6:2013-2018(1991). Braeuninger A.,et al.Proc. Natl. Acad. Sci. U.S.A. 88:10411-10415(1991). Brauninger A.,et al.Gene 110:205-211(1992). Braeuninger A.,et al.Oncogene 8:1365-1369(1993). Halleck A.,et al.Submitted (JUN-2004) to the EMBL/GenBank/DDBJ databases.

NCBI and Uniprot Product Information

• NCBI GI #: 187475373
• NCBI GeneID: 1445
• NCBI Accession #: NP_001120662.1
• NCBI GenBank Nucleotide #: NM_001127190.1
• UniProt Accession #: P41240; Q2M3N2; Q6FGZ6
• Molecular Weight: 50704
• NCBI Official Full Name: tyrosine-protein kinase CSK
• NCBI Official Synonym Full Names: c-src tyrosine kinase
• NCBI Official Symbol: CSK
• NCBI Protein Information: tyrosine-protein kinase CSK
• UniProt Protein Name: Tyrosine-protein kinase CSK
• Protein Family: Chloroplast sensor kinase
• UniProt Gene Name: CSK
• UniProt Entry Name: CSK_HUMAN

NCBI Description

CSK: a tyrosine kinase of the Csk family that phosphorylates and inhibits Src family kinases. Specifically phosphorylates Y504 on LCK, which acts as a negative regulatory site. Csk and the phospho-tyrosine phosphatase CD45 reciprocally regulate phosphorylation of the inhibitory tyrosine of the Src family kinases Lck and Fyn during T-cell receptor mediated signaling during thymic development.

Protein type: Kinase, protein; EC 2.7.10.2; Protein kinase, TK; Protein kinase, tyrosine (non-receptor); TK group; Csk family

Chromosomal Location of Human Ortholog: 15q24.1

Cellular Component: extrinsic to internal side of plasma membrane; cytoplasm; plasma membrane; intercellular junction; cytosol; lipid raft

Molecular Function: protein C-terminus binding; identical protein binding; protein binding; protein-tyrosine kinase activity; non-membrane spanning protein tyrosine kinase activity; protein phosphatase binding; ATP binding; receptor binding

Biological Process: epidermal growth factor receptor signaling pathway; negative regulation of phagocytosis; negative regulation of kinase activity; platelet activation; adaptive immune response; cell migration; negative regulation of Golgi to plasma membrane protein transport; protein amino acid phosphorylation; T cell receptor signaling pathway; regulation of cell proliferation; regulation of cytokine production; negative regulation of cell proliferation; positive regulation of MAP kinase activity; T cell costimulation; negative regulation of interleukin-6 production; morphogenesis of an epithelium; innate immune response; oligodendrocyte differentiation; brain development; blood coagulation; cell differentiation; negative regulation of bone resorption; transmembrane receptor protein tyrosine kinase signaling pathway

Uniprot Description

CSK: a tyrosine kinase of the Csk family that phosphorylates and inhibits Src family kinases. Specifically phosphorylates Y504 on LCK, which acts as a negative regulatory site. Csk and the phospho-tyrosine phosphatase CD45 reciprocally regulate phosphorylation of the inhibitory tyrosine of the Src family kinases Lck and Fyn during T-cell receptor mediated signaling during thymic development.

Protein type: Protein kinase, TK; Kinase, protein; EC 2.7.10.2; Protein kinase, tyrosine (non-receptor); TK group; Csk family

Chromosomal Location of Human Ortholog: 15q24.1

Cellular Component: cytoplasm; cytosol; extrinsic to internal side of plasma membrane; intercellular junction; lipid raft; plasma membrane

Molecular Function: ATP binding; identical protein binding; metal ion binding; non-membrane spanning protein tyrosine kinase activity; protein binding; protein C-terminus binding; protein phosphatase binding; protein-tyrosine kinase activity; receptor binding

Biological Process: adaptive immune response; blood coagulation; brain development; cell differentiation; cell migration; epidermal growth factor receptor signaling pathway; innate immune response; negative regulation of bone resorption; negative regulation of cell proliferation; negative regulation of Golgi to plasma membrane protein transport; negative regulation of interleukin-6 production; negative regulation of kinase activity; negative regulation of phagocytosis; oligodendrocyte differentiation; platelet activation; positive regulation of MAP kinase activity; protein amino acid phosphorylation; regulation of cell proliferation; regulation of cytokine production; T cell costimulation; T cell receptor signaling pathway; transmembrane receptor protein tyrosine kinase signaling pathway