Never In Mitosis Gene A Related Kinase 2 (NEK2) Recombinant Protein | NEK2 recombinant protein

Recombinant Never In Mitosis Gene A Related Kinase 2 (NEK2)

Cat. Num. AAA2009270

• Applications: SDS-Page, Western Blot, ELISA, Immunoprecipitation
• Purity: > 95%
• Synonyms: Never In Mitosis Gene A Related Kinase 2 (NEK2); Recombinant Never In Mitosis Gene A Related Kinase 2 (NEK2); NEK2 recombinant protein

• Host: E Coli
• Purity/Purification: > 95%
• Form/Format: Supplied as lyophilized form in PBS,pH7.4, containing 5% sucrose, 0.01% sarcosyl.
• Sequence: The target protein is fused with two N-terminal Tags, His-tag and T7-tag, its sequence is listed below.; MGSSHHHHHH SSGLVPRGSH MASMTGGQQM GRGS-VLHR DLKPANVFLD SKHNVKLGDF GLARILNHDT SFAKTFVGTPYYMSPEQISR LSYNEKSDIW SLGCLLYELC ALMPPFTAFN QKELAGKIRE GRFRRIPYRY SDGLNDLITR MLNLKDYHRP SVEEILESPL IADLVAEEQR RNLERRGRRS GEPSKLQDSSPVLSELKLKE RQLQDRERAL RAREDSLEQK ERELCIRERL AEDKLARAES LLKNYSLLKEHRLLCLAGGS ELDLPSSALK KKVHFHGESK ENTARSENSE
• Sequence Length: 443

• Applicable Applications for NEK2 recombinant protein: SDS-PAGE, Western Blot (WB), ELISA (EIA), Immunoprecipitation (IP)
• Organism: Rattus norvegicus (Rat)
• Expression System: Prokaryotic expression
• Residues: Val137~Glu400 (Accession # Q91XQ1) with two N-terminal Tags, His-tag and T7-tag
• Endotoxin Level: <1.0EU per 1ug (determined by the LAL method)
• Reconstitution: Reconstitute in sterile PBS, pH7.2-pH7.4.
• Preparation and Storage: Avoid repeated freeze/thaw cycles. Store at 2-8 degree C for one month. Aliquot and store at -80 degree C for 12 months.; Stability Test: The thermal stability is described by the loss rate of the targetprotein. The loss rate was determined by accelerated thermal degradation test,that is, incubate the protein at 37 degree C for 48h, and no obvious degradation andprecipitation were observed. (Referring from China Biological Products Standard,which was calculated by the Arrhenius equation.) The loss of this protein is lessthan 5% within the expiration date under appropriate storage condition.

SDS-Page

NCBI and Uniprot Product Information

• NCBI GI #: 281371462
• NCBI GeneID: 114482
• NCBI Accession #: NP_446143.1
• NCBI GenBank Nucleotide #: NM_053691.2
• UniProt Accession #: Q91XQ1
• Molecular Weight: 34.1kDa
• NCBI Official Full Name: serine/threonine-protein kinase Nek2
• NCBI Official Synonym Full Names: NIMA (never in mitosis gene a)-related expressed kinase 2
• NCBI Official Symbol: Nek2
• NCBI Protein Information: serine/threonine-protein kinase Nek2; NIMA-related kinase 2; NIMA (never in mitosis gene a)-related kinase 2
• UniProt Protein Name: NIMA related kinase 2
• Protein Family: Serine/threonine-protein kinase
• UniProt Gene Name: Nek2
• UniProt Entry Name: Q91XQ1_RAT

NCBI Description

mouse homolog displays cell cycle associated changes in nuclear and cytoplasmic localization; may act to regulate mitosis [RGD, Feb 2006]

Uniprot Description

NEK2: a protein kinase of the NEK family. Accumulates throughout S phase and shows maximal levels in late G2. This expression pattern is highly reminiscent of that of A and B cyclins. Phosphorylation of Hec1 by Nek2 is essential for faithful chromosome segregation

Protein type: Protein kinase, Other; EC 2.7.11.1; Protein kinase, Ser/Thr (non-receptor); Nucleolus; Kinase, protein; Other group; NEK family

Cellular Component: kinetochore; spindle pole; centrosome; intercellular bridge; condensed nuclear chromosome; protein complex; cytoplasm; midbody; nucleus

Molecular Function: protein serine/threonine kinase activity; protein phosphatase binding; ATP binding; protein kinase activity

Biological Process: regulation of attachment of spindle microtubules to kinetochore; blastocyst development; mitotic sister chromatid segregation; protein amino acid autophosphorylation; centrosome separation; protein amino acid phosphorylation; negative regulation of DNA binding; microtubule-based process; chromosome segregation