Rabbit anti-Human EIF4E Polyclonal Antibody | anti-EIF4E antibody

EIF4E (EIF4E, EIF4EL1, EIF4F, Eukaryotic translation initiation factor 4E, eIF-4F 25kD subunit, mRNA cap-binding protein) (Biotin)

Cat. Num. AAA6295085

• Gene Names: EIF4E; CBP; EIF4F; AUTS19; EIF4E1; EIF4EL1
• Reactivity: Human
• Applications: Flow Cytometry, Functional Assay, ELISA, Immunohistochemistry, Western Blot
• Purity: Purified by Protein A Affinity Chromatography.
• Synonyms: EIF4E; Polyclonal Antibody; EIF4E (EIF4E; EIF4EL1; EIF4F; Eukaryotic translation initiation factor 4E; eIF-4F 25kD subunit; mRNA cap-binding protein) (Biotin); mRNA cap-binding protein; anti-EIF4E antibody

• Host: Rabbit
• Reactivity: Human
• Clonality: Polyclonal
• Isotype: IgG
• Specificity: Recognizes human VGFR2.
• Purity/Purification: Purified by Protein A Affinity Chromatography.
• Form/Format: Supplied as a liquid in PBS, pH 7.2. No preservative added. Labeled with Biotin.

• Applicable Applications for anti-EIF4E antibody: Flow Cytometry (FC/FACS), ELISA (EIA), Immunohistochemistry (IHC), Western Blot (WB)
• Application Notes: FC/FACS: 1:10-50; ELISA: 1:1,000; IHC: 1:50-100; WB: 1:100-500; Applications are based on unconjugated antibody.
• Immunogen: Recombinant protein corresponding to human VGFR2
• Conjugate: Biotin
• Note: Preservative Free
• Preparation and Storage: Store product at 4 degree C if to be used immediately within two weeks. For long-term storage, aliquot to avoid repeated freezing and thawing and store at -20 degree C. Aliquots are stable at -20 degree C for 12 months after receipt. Dilute required amount only prior to immediate use. Further dilutions can be made in assay buffer. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.

Western Blot (WB)

(Western Blot analysis of HL-60 cell line lysates (35ug/lane) using 035012.)

Immunohistochemistry (IHC)

(Formalin-fixed and paraffin-embedded human testis tissue reacted with 035012, which was peroxidase-conjugated to the secondary antibody, followed by DAB staining.)

Flow Cytometry (FC/FACS)

(Flow cytometric analysis with HL-60 cells (bottom histogram) using 035012 compared to a negative control cell (top histogram). FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.)

Related Product Information for anti-EIF4E antibody

EIF4E is a eukaryotic translation initiation factor involved in directing ribosomes to the cap structure of mRNAs. It is a 24-kD polypeptide that exists as both a free form and as part of a multiprotein complex termed EIF4F. The EIF4E polypeptide is the rate-limiting component of the eukaryotic translation apparatus and is involved in the mRNA-ribosome binding step of eukaryotic protein synthesis. The other subunits of EIF4F are a 50-kD polypeptide, termed EIF4A (see MIM 601102), that possesses ATPase and RNA helicase activities, and a 220-kD polypeptide, EIF4G.

Product Categories/Family for anti-EIF4E antibody

Antibodies; Translation Initiation Factors

References

Kim, S., et.al., J. Virol. 84 (1), 52-58 (2010). Kao, C.L., et.al., Cancer Lett. 286 (2), 250-259 (2009).

NCBI and Uniprot Product Information

• NCBI GI #: 194578907
• NCBI GeneID: 1977
• NCBI Accession #: NP_001124150.1
• NCBI GenBank Nucleotide #: NM_001130678.1
• UniProt Accession #: P06730; Q96E95; B7Z6V1; D6RCQ6
• Molecular Weight: 25,097 Da
• NCBI Official Full Name: eukaryotic translation initiation factor 4E isoform 3
• NCBI Official Synonym Full Names: eukaryotic translation initiation factor 4E
• NCBI Official Symbol: EIF4E
• NCBI Official Synonym Symbols: CBP; EIF4F; AUTS19; EIF4E1; EIF4EL1
• NCBI Protein Information: eukaryotic translation initiation factor 4E; eIF-4E; eIF-4F 25 kDa subunit; mRNA cap-binding protein; eukaryotic translation initiation factor 4E-like 1
• UniProt Protein Name: Eukaryotic translation initiation factor 4E
• Protein Family: Eukaryotic translation initiation factor
• UniProt Gene Name: EIF4E
• UniProt Synonym Gene Names: EIF4EL1; EIF4F; eIF-4E; eIF4E
• UniProt Entry Name: IF4E_HUMAN

NCBI Description

The protein encoded by this gene is a component of the eukaryotic translation initiation factor 4F complex, which recognizes the 7-methylguanosine cap structure at the 5' end of cellular mRNAs. The encoded protein aids in translation initiation by recruiting ribosomes to the mRNA. Association of this protein with the 4F complex is the rate-limiting step in translation initiation. Three transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, Jan 2012]

Uniprot Description

EIF4E: a protein of the eukaryotic initiation factor 4E family. Recognizes and binds the 7-methylguanosine-containing mRNA cap during an early step in the initiation of protein synthesis and facilitates ribosome binding by inducing the unwinding of the mRNAs secondary structures. Phosphorylation increases the ability of the protein to bind to mRNA caps and to form the EIF4F complex.

Protein type: Translation initiation; Translation

Chromosomal Location of Human Ortholog: 4q23

Cellular Component: mRNA cap complex; stress granule; cytoplasm; eukaryotic translation initiation factor 4F complex; cytosol

Molecular Function: protein binding; eukaryotic initiation factor 4G binding; translation initiation factor activity; RNA cap binding

Biological Process: translation; viral reproduction; cytokine and chemokine mediated signaling pathway; positive regulation of mitotic cell cycle; regulation of translation; poly(A) tail shortening; mRNA export from nucleus; cellular protein metabolic process; insulin receptor signaling pathway; translational initiation; gene expression; mRNA catabolic process, deadenylation-dependent decay; lung development; G1/S transition of mitotic cell cycle

Disease: Autism, Susceptibility To, 19