Western Blot (WB)
(Western Blot: Sample: Recombinant protein.)
Rabbit anti-Rat Leukocyte Cell Derived Chemotaxin 2 (LECT2) Polyclonal Antibody | anti-LECT2 antibody
Polyclonal Antibody to Leukocyte Cell Derived Chemotaxin 2 (LECT2)
Reactivity
Rat
Applications
Immunocytochemistry, Immunohistochemistry, ELISA, Western Blot
Purity
Affinity Chromatography
Synonyms
Leukocyte Cell Derived Chemotaxin 2 (LECT2); Polyclonal Antibody; Polyclonal Antibody to Leukocyte Cell Derived Chemotaxin 2 (LECT2); anti-LECT2 antibody
Host
Rabbit
Reactivity
Rat
Clonality
Polyclonal
Specificity
The antibody is a rabbit polyclonal antibody raised against LECT2. It has been selected for its ability to recognize LECT2 in immunohistochemical staining andwestern blotting.
Purity/Purification
Affinity Chromatography
Form/Format
Supplied as solution form in PBS, pH7.4, containing 0.02% NaN3,50% glycerol.
Concentration
500ug/mL (varies by lot)
Sequence
Antigen: The target protein is fused with two N-terminal Tags, His-tag and its sequence is listed below.
MGSSHHHHHH SSGLVPRGSH MASMTGGQQM GRGSEF-MFPTRILMSA ALISTALAGP WANICASRSS NEIRTCDSYG CGQYSTQRTQ RHHPGVDVLC SDGSVVYAPF TGKIVGQEKP YRNKNAINDG VRLSGRGFCV KIFYIKPIKY KGSIRKGEKL GTLLPLQKVY PGIQSHVHIE NCDSSDPTAY L
MGSSHHHHHH SSGLVPRGSH MASMTGGQQM GRGSEF-MFPTRILMSA ALISTALAGP WANICASRSS NEIRTCDSYG CGQYSTQRTQ RHHPGVDVLC SDGSVVYAPF TGKIVGQEKP YRNKNAINDG VRLSGRGFCV KIFYIKPIKY KGSIRKGEKL GTLLPLQKVY PGIQSHVHIE NCDSSDPTAY L
Sequence Length
151
Applicable Applications for anti-LECT2 antibody
Immunocytochemistry (ICC), Immunohistochemistry (IHC) - Formalin/Paraffin, ELISA (EIA), Western Blot (WB)
Application Notes
Western blotting: 1:100-400
Immunocytochemistry in formalin fixed cells: 1:100-500
Immunohistochemistry in formalin fixed frozen section: 1:100-500
Immunohistochemistry in paraffin section: 1:50-200
Enzyme-linked Immunosorbent Assay: 1:100-200
Immunocytochemistry in formalin fixed cells: 1:100-500
Immunohistochemistry in formalin fixed frozen section: 1:100-500
Immunohistochemistry in paraffin section: 1:50-200
Enzyme-linked Immunosorbent Assay: 1:100-200
Immunogen
Recombinant LECT2 (Met1~Leu151) expressed in E.coli.
Cross Reactivity
Rat
Conjugated Antibody
The APC conjugated antibody version of this item is also available as catalog #MBS2076760
Preparation and Storage
Store at 4 degree C for frequent use. Stored at -20 degree C to -80 degree C in a manual defrost freezer for one year without detectable loss of activity. Avoid repeated freeze-thaw cycles.
Western Blot (WB)
(Western Blot: Sample: Recombinant protein.)
Western Blot (WB)
(Western Blot: Sample: Recombinant protein.)
Immunohistochemistry (IHC)
(DABstainingonIHC-P.Samples:RatTissue))
Immunohistochemistry (IHC)
(DABstainingonIHC-P.Samples:RatTissue))
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
UniProt Accession #
Molecular Weight
16,598 Da
NCBI Official Full Name
leukocyte cell-derived chemotaxin-2
NCBI Official Synonym Full Names
leukocyte cell-derived chemotaxin 2
NCBI Official Symbol
Lect2
NCBI Protein Information
leukocyte cell-derived chemotaxin-2
UniProt Protein Name
Leukocyte cell-derived chemotaxin 2
Protein Family
UniProt Gene Name
Lect2
NCBI Description
This gene encodes a secreted, 16 kDa protein that acts as a chemotactic factor to neutrophils and stimulates the growth of chondrocytes and osteoblasts. This protein has high sequence similarity to the chondromodulin repeat regions of the chicken myb-induced myeloid 1 protein. A polymorphism in this gene may be associated with rheumatoid arthritis. [provided by RefSeq, Jul 2008]
Uniprot Description
LECT2: Has a neutrophil chemotactic activity. Also a positive regulator of chondrocyte proliferation. Belongs to the LECT2/MIM-1 family.
Protein type: Secreted, signal peptide; Secreted; Motility/polarity/chemotaxis
Chromosomal Location of Human Ortholog: 5q31.1
Cellular Component: extracellular space; cytoplasm
Molecular Function: identical protein binding
Biological Process: negative regulation of Wnt receptor signaling pathway; chemotaxis; skeletal development
Research Articles on LECT2
Similar Products
Product Notes
The LECT2 lect2 (Catalog #AAA2006681) is an Antibody produced from Rabbit and is intended for research purposes only. The product is available for immediate purchase. The Polyclonal Antibody to Leukocyte Cell Derived Chemotaxin 2 (LECT2) reacts with Rat and may cross-react with other species as described in the data sheet. AAA Biotech's Leukocyte Cell Derived Chemotaxin 2 (LECT2) can be used in a range of immunoassay formats including, but not limited to, Immunocytochemistry (ICC), Immunohistochemistry (IHC) - Formalin/Paraffin, ELISA (EIA), Western Blot (WB). Western blotting: 1:100-400 Immunocytochemistry in formalin fixed cells: 1:100-500 Immunohistochemistry in formalin fixed frozen section: 1:100-500 Immunohistochemistry in paraffin section: 1:50-200 Enzyme-linked Immunosorbent Assay: 1:100-200. Researchers should empirically determine the suitability of the LECT2 lect2 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. The amino acid sequence is listed below: Antigen: The target protein is fused with two N-terminal Tags, His-tag and its sequence is listed below. MGSSHHHHHH SSGLVPRGSH MASMTGGQQM GRGSEF-MFP TRILMSA ALISTALAGP WANICASRSS NEIRTCDSYG CGQYSTQRTQ RHHPGVDVLC SDGSVVYAPF TGKIVGQEKP YRNKNAINDG VRLSGRGFCV KIFYIKPIKY KGSIRKGEKL GTLLPLQKVY PGIQSHVHIE NCDSSDPTAY L. It is sometimes possible for the material contained within the vial of "Leukocyte Cell Derived Chemotaxin 2 (LECT2), Polyclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
If you are ready to order, navigate to Shopping Cart and get ready to checkout.
