Western Blot (WB)
(Western Blot: Sample: Recombinant protein.)
Rabbit anti-Mouse Mitogen Activated Protein Kinase 8 (MAPK8) Polyclonal Antibody | anti-MAPK8 antibody
Polyclonal Antibody to Mitogen Activated Protein Kinase 8 (MAPK8)
MGSSHHHHHH SSGLVPRGSH MASMTGGQQM GRGS- GIK HLHSAGIIHR DLKPSNIVVK SDCTLKILDF GLARTAGTSF MMTPYVVTRY YRAPEVILGM GYKENVDLWS VGCIMGEMVC HKILFPGRDY IDQWNKVIEQ LGTPCPEFMK KLQPTVRTYV ENRPKYAGYS FEKLFPDVLF PADSEHNKLK ASQARDLLSK MLVIDASKRI SVDEALQHPY INVWYDPSEA EAPPPKIPDK QLDEREHTIE EWKELIYKEV MDLEERTKNG VIRGQPSPLA QV
Immunocytochemistry in formalin fixed cells: 1:100-500
Immunohistochemistry in formalin fixed frozen section: 1:100-500
Immunohistochemistry in paraffin section: 1:50-200
Enzyme-linked Immunosorbent Assay: 1:100-200
Western Blot (WB)
(Western Blot: Sample: Recombinant protein.)
Western Blot (WB)
(Western Blot: Sample: Recombinant protein.)
Immunohistochemistry (IHC)
(DABstainingonIHC-P.Samples:MouseTissue))
Immunohistochemistry (IHC)
(DABstainingonIHC-P.Samples:MouseTissue))
NCBI and Uniprot Product Information
Uniprot Description
JNK1: a protein kinase of the MAPK family that is potently activated by a variety of environmental stresses, including UV and gamma radiation, ceramides, pro-inflammatory cytokines and, in some instances, by growth factors and GPCR agonists. Substrates include a number of transcription factors, primarily components of AP-1 such as c-Jun and ATF2, thus regulating AP-1 transcriptional activity. In T- cells, JNK1 and JNK2 are required for polarized differentiation of T-helper cells into Th1 cells. Binds to at least four scaffolding proteins, JIP-1, -2, -3 and -4. Activity increased in obesity. Inhibition or mouse knockout increases insulin sensitivity. Part of NFB pathway involved in inflammation and cancer, and signals downstream of Ras, though possibly as an apoptotic negative regulator of growth. Four alternatively-spliced isoforms have been described.
Protein type: CMGC group; EC 2.7.11.24; JNK subfamily; Kinase, protein; MAPK family; MAPK/JNK subfamily; Protein kinase, CMGC; Protein kinase, Ser/Thr (non-receptor)
Chromosomal Location of Human Ortholog: 14|14 B
Cellular Component: axon; cytoplasm; cytosol; dendrite cytoplasm; mitochondrion; neuron projection; nucleus; perikaryon; podosome; vesicle
Molecular Function: ATP binding; enzyme binding; histone deacetylase binding; histone deacetylase regulator activity; JUN kinase activity; kinase activity; kinesin binding; MAP kinase activity; nucleotide binding; protein binding; protein kinase activity; protein serine/threonine kinase activity; transferase activity
Biological Process: cellular response to amino acid starvation; cellular response to cadmium ion; cellular response to hydrogen peroxide; cellular response to nitric oxide; cellular response to organic substance; cellular response to reactive oxygen species; dendrite morphogenesis; determination of dorsal identity; inflammatory response; JNK cascade; JUN phosphorylation; negative regulation of apoptosis; negative regulation of protein binding; neuron migration; neuron projection development; ossification; peptidyl-serine phosphorylation; peptidyl-threonine phosphorylation; phosphorylation; positive regulation of apoptotic signaling pathway; positive regulation of cardiac muscle cell apoptotic process; positive regulation of cell migration; positive regulation of cyclase activity; positive regulation of deacetylase activity; positive regulation of determination of dorsal identity; positive regulation of DNA replication; positive regulation of gene expression; positive regulation of microtubule polymerization; positive regulation of neuroblast proliferation; positive regulation of neuron apoptotic process; positive regulation of neuron migration; positive regulation of podosome assembly; positive regulation of protein metabolic process; programmed necrotic cell death; protein amino acid phosphorylation; protein localization to tricellular tight junction; regulation of centrosome cycle; regulation of circadian rhythm; regulation of DNA replication origin binding; regulation of gene expression; regulation of neuron differentiation; regulation of protein localization; regulation of transcription, DNA-templated; response to cadmium ion; response to heat; response to hydrogen peroxide; response to mechanical stimulus; response to osmotic stress; response to UV; rhythmic process; signal transduction; stress-activated MAPK cascade
Research Articles on MAPK8
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Product Notes
The MAPK8 mapk8 (Catalog #AAA2003601) is an Antibody produced from Rabbit and is intended for research purposes only. The product is available for immediate purchase. The Polyclonal Antibody to Mitogen Activated Protein Kinase 8 (MAPK8) reacts with Mouse and may cross-react with other species as described in the data sheet. AAA Biotech's Mitogen Activated Protein Kinase 8 (MAPK8) can be used in a range of immunoassay formats including, but not limited to, Immunocytochemistry (ICC), Immunohistochemistry (IHC) - Formalin/Paraffin, ELISA (EIA), Western Blot (WB). Western blotting: 1:100-400 Immunocytochemistry in formalin fixed cells: 1:100-500 Immunohistochemistry in formalin fixed frozen section: 1:100-500 Immunohistochemistry in paraffin section: 1:50-200 Enzyme-linked Immunosorbent Assay: 1:100-200. Researchers should empirically determine the suitability of the MAPK8 mapk8 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. The amino acid sequence is listed below: Antigen: The target protein is fused with two N-terminal Tags, His-tag and its sequence is listed below. MGSSHHHHHH SSGLVPRGSH MASMTGGQQM GRGS- GIK HLHSAGIIHR DLKPSNIVVK SDCTLKILDF GLARTAGTSF MMTPYVVTRY YRAPEVILGM GYKENVDLWS VGCIMGEMVC HKILFPGRDY IDQWNKVIEQ LGTPCPEFMK KLQPTVRTYV ENRPKYAGYS FEKLFPDVLF PADSEHNKLK ASQARDLLSK MLVIDASKRI SVDEALQHPY INVWYDPSEA EAPPPKIPDK QLDEREHTIE EWKELIYKEV MDLEERTKNG VIRGQPSPLA QV. It is sometimes possible for the material contained within the vial of "Mitogen Activated Protein Kinase 8 (MAPK8), Polyclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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