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Western Blot (WB) (Western Blot: Sample: Recombinant protein.)

Rabbit anti-Rat Vascular Endothelial Growth Factor B (VEGFB) Polyclonal Antibody | anti-VEGFB antibody

Polyclonal Antibody to Vascular Endothelial Growth Factor B (VEGFB)

Reactivity
Rat
Applications
Immunocytochemistry, Immunohistochemistry, ELISA, Western Blot
Purity
Affinity Chromatography
Synonyms
Vascular Endothelial Growth Factor B (VEGFB); Polyclonal Antibody; Polyclonal Antibody to Vascular Endothelial Growth Factor B (VEGFB); anti-VEGFB antibody
Ordering
For Research Use Only!
Host
Rabbit
Reactivity
Rat
Clonality
Polyclonal
Specificity
The antibody is a rabbit polyclonal antibody raised against VEGFB. It has been selected for its ability to recognize VEGFB in immunohistochemical staining andwestern blotting.
Purity/Purification
Affinity Chromatography
Form/Format
Supplied as solution form in PBS, pH7.4, containing 0.02% NaN3,50% glycerol.
Concentration
500ug/mL (varies by lot)
Sequence
Antigen: The target protein is fused with two N-terminal Tags, His-tag and its sequence is listed below.
MGSSHHHHHH SSGLVPRGSH MASMTGGQQM GRGSEF-APVSQFDGPS HQKKVVSWID VYARATCQPR EVVVPLSMEL MGNVVKQLVP SCVTVQRCGG CCPDDGLECV PIGQHQVRMQ ILMIQYPSSQ LGEMSLEEHS QCECRPKRKE SAVKPDS
Sequence Length
207
Applicable Applications for anti-VEGFB antibody
Immunocytochemistry (ICC), Immunohistochemistry (IHC) - Formalin/Paraffin, ELISA (EIA), Western Blot (WB)
Application Notes
Western blotting: 1:100-400
Immunocytochemistry in formalin fixed cells: 1:100-500
Immunohistochemistry in formalin fixed frozen section: 1:100-500
Immunohistochemistry in paraffin section: 1:50-200
Enzyme-linked Immunosorbent Assay: 1:100-200
Immunogen
Recombinant VEGFB (Ala21~Ser137) expressed in E.coli.
Cross Reactivity
Rat
Conjugated Antibody
The APC conjugated antibody version of this item is also available as catalog #MBS2038768
Preparation and Storage
Store at 4 degree C for frequent use. Stored at -20 degree C to -80 degree C in a manual defrost freezer for one year without detectable loss of activity. Avoid repeated freeze-thaw cycles.

Western Blot (WB)

(Western Blot: Sample: Recombinant protein.)

Western Blot (WB) (Western Blot: Sample: Recombinant protein.)

Immunohistochemistry (IHC)

(DABstainingonIHC-P.Samples:RatTissue))

Immunohistochemistry (IHC) (DABstainingonIHC-P.Samples:RatTissue))

NCBI and Uniprot Product Information

NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
UniProt Accession #
Molecular Weight
21,102 Da
NCBI Official Full Name
vascular endothelial growth factor B
NCBI Official Synonym Full Names
vascular endothelial growth factor B
NCBI Official Symbol
Vegfb
NCBI Protein Information
vascular endothelial growth factor B
UniProt Protein Name
Vascular endothelial growth factor B
UniProt Gene Name
Vegfb
UniProt Synonym Gene Names
Vrf; VEGF-B; VRF

NCBI Description

mouse homolog is a growth factor; involved in the promotion of angiogenesis [RGD, Feb 2006]

Uniprot Description

VEGFB: Growth factor for endothelial cells. VEGF-B167 binds heparin and neuropilin-1 whereas the binding to neuropilin-1 of VEGF-B186 is regulated by proteolysis. Homodimer; disulfide-linked. Can also form heterodimer with VEGF. Expressed in all tissues except liver. Highest levels found in heart, skeletal muscle and pancreas. Belongs to the PDGF/VEGF growth factor family. 2 isoforms of the human protein are produced by alternative splicing.

Protein type: Cell cycle regulation; Cytokine; Motility/polarity/chemotaxis; Secreted; Secreted, signal peptide

Chromosomal Location of Human Ortholog: 1q43

Cellular Component: extracellular region; extracellular space; intracellular; membrane

Molecular Function: chemoattractant activity; growth factor activity; heparin binding; protein heterodimerization activity; protein homodimerization activity; vascular endothelial growth factor receptor 1 binding; vascular endothelial growth factor receptor 2 binding

Biological Process: angiogenesis; cardiac muscle contraction; heart development; induction of positive chemotaxis; negative regulation of apoptosis; negative regulation of neuron apoptosis; positive regulation of angiogenesis; positive regulation of cell division; positive regulation of endothelial cell proliferation; positive regulation of peptidyl-tyrosine phosphorylation; positive regulation of protein kinase B signaling; positive regulation of vascular endothelial growth factor receptor signaling pathway; positive regulation of vascular permeability; protein O-linked glycosylation; response to drug; response to hypoxia; vascular endothelial growth factor receptor signaling pathway

Research Articles on VEGFB

Similar Products

Product Notes

The VEGFB vegfb (Catalog #AAA2005459) is an Antibody produced from Rabbit and is intended for research purposes only. The product is available for immediate purchase. The Polyclonal Antibody to Vascular Endothelial Growth Factor B (VEGFB) reacts with Rat and may cross-react with other species as described in the data sheet. AAA Biotech's Vascular Endothelial Growth Factor B (VEGFB) can be used in a range of immunoassay formats including, but not limited to, Immunocytochemistry (ICC), Immunohistochemistry (IHC) - Formalin/Paraffin, ELISA (EIA), Western Blot (WB). Western blotting: 1:100-400 Immunocytochemistry in formalin fixed cells: 1:100-500 Immunohistochemistry in formalin fixed frozen section: 1:100-500 Immunohistochemistry in paraffin section: 1:50-200 Enzyme-linked Immunosorbent Assay: 1:100-200. Researchers should empirically determine the suitability of the VEGFB vegfb for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. The amino acid sequence is listed below: Antigen: The target protein is fused with two N-terminal Tags, His-tag and its sequence is listed below. MGSSHHHHHH SSGLVPRGSH MASMTGGQQM GRGSEF-APV SQFDGPS HQKKVVSWID VYARATCQPR EVVVPLSMEL MGNVVKQLVP SCVTVQRCGG CCPDDGLECV PIGQHQVRMQ ILMIQYPSSQ LGEMSLEEHS QCECRPKRKE SAVKPDS. It is sometimes possible for the material contained within the vial of "Vascular Endothelial Growth Factor B (VEGFB), Polyclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.

Precautions

All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.

Disclaimer

Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.

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