Western Blot (WB)
(Anti-BOB1 antibody, MBS175383, Western blottingLane: Rat Spleen Tissue Lysate )
Rabbit anti-Mouse, Rat BOB1 Polyclonal Antibody | anti-POU2AF1 antibody
Anti-BOB1 antibody
Gene Names
Pou2af1; Bob1; OCAB; BOB.1; Bob-1; OBF-1; OBF.1; OCA-B
Reactivity
Mouse, Rat
Applications
Western Blot, Immunohistochemistry
Purity
Immunogen affinity purified.
Synonyms
BOB1; Polyclonal Antibody; Anti-BOB1 antibody; POU domain class 2-associating factor 1; POU class 2 associating factor 1; B cell Oct binding protein 1 antibody; B cell specific coactivator OBF 1 antibody; B cell specific coactivator OBF1 antibody; B-cell-specific coactivator OBF-1 antibody; BOB 1 antibody; BOB-1 antibody; OBF 1 antibody; OBF1 antibody; OBF1_HUMAN antibody; OCA B antibody; OCA-B antibody; OCAB antibody; OCT binding factor 1 antibody; OCT-binding factor 1 antibody; POU domain class 2 associating factor 1 antibody; POU domain class 2-associating factor 1 antibody; POU2AF1 antibody; anti-POU2AF1 antibody
Host
Rabbit
Reactivity
Mouse, Rat
Clonality
Polyclonal
Isotype
IgG
Purity/Purification
Immunogen affinity purified.
Form/Format
Lyophilized
Sequence Length
256
Applicable Applications for anti-POU2AF1 antibody
Western Blot (WB), Immunohistochemistry (IHC) Paraffin
Immunogen
A synthetic peptide corresponding to a sequence at the C-terminus of mouse BOB1 (238-256aa EEEESNTYELNHTLSVEGF), different from the related rat sequence by one amino acid, and from human sequence by four amino acids.
Contents
Each vial contains 5mg BSA, 0.9mg NaCl, 0.2mg Na2HPO4, 0.05mg Thimerosal, 0.05mg NaN3.
Reconstitution
Add 0.2ml of distilled water will yield a concentration of 500ug/ml.
Preparation and Storage
At -20 degree C for one year. After reconstitution, at 4 degree C for one month. It can also be aliquotted and stored frozen at -20 degree C for a longer time. Avoid repeated freezing and thawing.
Western Blot (WB)
(Anti-BOB1 antibody, MBS175383, Western blottingLane: Rat Spleen Tissue Lysate )
Western Blot (WB)
(Anti-BOB1 antibody, MBS175383, Western blottingLane: Rat Spleen Tissue Lysate )
Immunohistochemistry (IHC)
(Anti-BOB1 antibody, MBS175383, IHC(P)IHC(P): Rat Tonsil Tissue )
Immunohistochemistry (IHC)
(Anti-BOB1 antibody, MBS175383, IHC(P)IHC(P): Rat Tonsil Tissue )
Related Product Information for anti-POU2AF1 antibody
Description: Rabbit IgG polyclonal antibody for POU domain class 2-associating factor 1(POU2AF1) detection. Tested with WB, IHC-P in Mouse, Rat.
Background: POU2AF1(POU domain class 2-associating factor 1), also known as OBF1, BOB1 and OCAB, is a protein that in humans is encoded by the POU2AF1 gene. POU domain proteins contain a bipartite DNA-binding domain divided by a flexible linker that enables them to adopt various monomer configurations on DNA. The versatility of POU protein operation is additionally conferred at the dimerization level. Casellas et al.(2002) reported that OCAB is essential for V(D)J recombination of a subset of Ig-kappa genes. They showed that OCAB modulates recombination by directly enhancing Ig-kappa gene transcription in vivo. Siegel et al.(2006) suggested that, together with dysregulation of other OCAB target genes, altered regulation of SYK may help explain the magnitude of defects observed in B-cell development, including the pre-B1-to-pre-B2 transition, and immune responses in Ocab -/- mice.
Background: POU2AF1(POU domain class 2-associating factor 1), also known as OBF1, BOB1 and OCAB, is a protein that in humans is encoded by the POU2AF1 gene. POU domain proteins contain a bipartite DNA-binding domain divided by a flexible linker that enables them to adopt various monomer configurations on DNA. The versatility of POU protein operation is additionally conferred at the dimerization level. Casellas et al.(2002) reported that OCAB is essential for V(D)J recombination of a subset of Ig-kappa genes. They showed that OCAB modulates recombination by directly enhancing Ig-kappa gene transcription in vivo. Siegel et al.(2006) suggested that, together with dysregulation of other OCAB target genes, altered regulation of SYK may help explain the magnitude of defects observed in B-cell development, including the pre-B1-to-pre-B2 transition, and immune responses in Ocab -/- mice.
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
UniProt Accession #
Molecular Weight
27,692 Da
NCBI Official Full Name
POU domain class 2-associating factor 1
NCBI Official Synonym Full Names
POU domain, class 2, associating factor 1
NCBI Official Symbol
Pou2af1
NCBI Official Synonym Symbols
Bob1; OCAB; BOB.1; Bob-1; OBF-1; OBF.1; OCA-B
NCBI Protein Information
POU domain class 2-associating factor 1; OCT-binding factor 1; B-cell-specific coactivator OBF-1
UniProt Protein Name
POU domain class 2-associating factor 1
Protein Family
UniProt Gene Name
Pou2af1
UniProt Synonym Gene Names
Obf-1; BOB1
UniProt Entry Name
OBF1_MOUSE
Uniprot Description
POU2AF1: Transcriptional coactivator that specifically associates with either OCT1 or OCT2. It boosts the OCT1 mediated promoter activity and to a lesser extent, that of OCT2. It has no intrinsic DNA-binding activity. It recognizes the POU domains of OCT1 and OCT2. It is essential for the response of B-cells to antigens and required for the formation of germinal centers. A chromosomal aberration involving POU2AF1/OBF1 may be a cause of a form of B-cell leukemia. Translocation t(3;11)(q27;q23) with BCL6. Belongs to the POU2AF1 family.
Protein type: Transcription, coactivator/corepressor; Oncoprotein
Cellular Component: nucleus
Molecular Function: protein binding; DNA binding
Biological Process: transcription, DNA-dependent; regulation of transcription, DNA-dependent
Research Articles on POU2AF1
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Product Notes
The POU2AF1 pou2af1 (Catalog #AAA175383) is an Antibody produced from Rabbit and is intended for research purposes only. The product is available for immediate purchase. The Anti-BOB1 antibody reacts with Mouse, Rat and may cross-react with other species as described in the data sheet. AAA Biotech's BOB1 can be used in a range of immunoassay formats including, but not limited to, Western Blot (WB), Immunohistochemistry (IHC) Paraffin. Researchers should empirically determine the suitability of the POU2AF1 pou2af1 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "BOB1, Polyclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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