Rabbit anti-Rat Olig2 (Ser10/13/14) Polyclonal Antibody | anti-OLIG2 antibody

Anti-Phospho-Ser10,13,14 Olig2

Cat. Num. AAA502203

• Gene Names: OLIG2; BHLHB1; OLIGO2; RACK17; PRKCBP2; bHLHe19
• Reactivity: Rat
• Applications: Western Blot
• Purity: Affinity Purified (Prepared from rabbit serum by affinity purification via sequential chromatography on phospho- and dephosphopeptide affinity columns.)
• Synonyms: Olig2 (Ser10/13/14); Polyclonal Antibody; Anti-Phospho-Ser10; 13; 14 Olig2; anti-OLIG2 antibody

• Host: Rabbit
• Reactivity: Rat
• Clonality: Polyclonal
• Specificity: Specific for the ~32k Olig2 phosphorylated at Ser10, Ser13, and Ser14.
• Purity/Purification: Affinity Purified (Prepared from rabbit serum by affinity purification via sequential chromatography on phospho- and dephosphopeptide affinity columns.)
• Form/Format: 100 ul in 10 mM HEPES (pH 7.5), 150 mM NaCl, 100 ug BSA per ml and 50% glycerol. Adequate amount of material to conduct 10-mini Western Blots.
• Sequence Length: 323

• Applicable Applications for anti-OLIG2 antibody: Western Blot (WB)
• Application Notes: Quality Control: Western bots performed on each lot.; WB: 1:1000
• Antigen: Phosphopeptide corresponding to amino acid residues surrounding the phospho-Ser10,13,14 of Olig2.
• Immunogen Information: Synthetic phospho-peptide corresponding to amino acid residues surrounding Ser10,13,14 conjugated to KLH
• Immunogen Species: Human
• Reactivity Assumed Based on 100% Sequence Homology: Guinea pig, human, mouse, non-human primate, zebrafish
• Species Reactivity Note: The antibody has been directly tested for reactivity in Western blots with rat tissue. It is anticipated that the antibody will react with guinea pig, human, mouse, non-human primate, and Zebrafish based on the fact that these species have 100% homology with the amino acid sequence used as antigen.
• Biological Significance: Olig2 is a well conserved bHLH transcription factor that shows both anti-neural functions and pro-neural functions at different stages in the formation of the oligodendrocyte lineage (Sun et al., 2011). Olig2 is expressed in 100% of the human diffuse gliomas irrespective of grade and required for intracranial tumor formation in a genetically relevant model of malignant glioma (Ligon et al., 2004; Ligon et al., 2007). A developmentally regulated triple serine motif at positions 10, 13 and 14 in the amino terminus is well conserved across species ranging from humans to zebrafish and is essential for Olig2 proliferative function in both normal and malignant neural progenitors (Sun et al., 2011). All three serine residues must be mutated to achieve a strong loss-of-function or gain-of-function phenotype, suggesting that the phosphorylation state of Olig2 represents a significant conformational change in the amino terminus (Sun et al., 2011).
• Preparation and Storage: For long term storage -20 degree C is recommended. Stable at -20 degree C for at least 1 year

Testing Data

Related Product Information for anti-OLIG2 antibody

Affinity purified rabbit polyclonal antibody

References

• Ligon KL, Alberta JA, Kho AT, Weiss J, Kwaan MR, Nutt CL, Louis DN, Stiles CD, Rowitch DH. The oligodendroglial lineage marker OLIG2 is universally expressed in diffuse gliomas. J Neuropathol Exp Neurol. 2004;63:499-509
• Ligon KL, Huillard E, Mehta S, Kesari S, Liu H, Alberta JA, Bachoo RM, Kane M, Louis DN, Depinho RA, Anderson DJ, Stiles CD, Rowitch DH. (2007) Olig2-regulated lineage-restricted pathway controls replication competence in neural stem cells and malignant glioma. Neuron. 2007;53:503-17.
• Sun Y, Meijer DH, Alberta JA, Mehta S, Kane MF, Tien AC, Fu H, Petryniak MA, Potter GB, Liu Z, Powers JF, Runquist IS, Rowitch DH, Stiles CD. (2011) Phosphorylation state of Olig2 regulates proliferation of neural progenitors. Neuron 69(5):906-17

NCBI and Uniprot Product Information

• NCBI GI #: 17978475
• NCBI GeneID: 10215
• NCBI Accession #: NP_005797.1
• NCBI GenBank Nucleotide #: NM_005806.3
• UniProt Accession #: Q13516; Q86X04; Q9NZ14; B3KRF3
• Molecular Weight: 32
• NCBI Official Full Name: oligodendrocyte transcription factor 2
• NCBI Official Synonym Full Names: oligodendrocyte lineage transcription factor 2
• NCBI Official Symbol: OLIG2
• NCBI Official Synonym Symbols: BHLHB1; OLIGO2; RACK17; PRKCBP2; bHLHe19
• NCBI Protein Information: oligodendrocyte transcription factor 2; protein kinase C-binding protein 2; class B basic helix-loop-helix protein 1; class E basic helix-loop-helix protein 19; human protein kinase C-binding protein RACK17; basic domain, helix-loop-helix protein, class B, 1; oligodendrocyte-specific bHLH transcription factor 2
• UniProt Protein Name: Oligodendrocyte transcription factor 2
• Protein Family: Oligodendrocyte transcription factor
• UniProt Gene Name: OLIG2
• UniProt Synonym Gene Names: BHLHB1; BHLHE19; PRKCBP2; RACK17; Oligo2; bHLHb1; bHLHe19
• UniProt Entry Name: OLIG2_HUMAN

NCBI Description

This gene encodes a basic helix-loop-helix transcription factor which is expressed in oligodendroglial tumors of the brain. The protein is an essential regulator of ventral neuroectodermal progenitor cell fate. The gene is involved in a chromosomal translocation t(14;21)(q11.2;q22) associated with T-cell acute lymphoblastic leukemia. Its chromosomal location is within a region of chromosome 21 which has been suggested to play a role in learning deficits associated with Down syndrome. [provided by RefSeq, Jul 2008]

Uniprot Description

Function: Required for oligodendrocyte and motor neuron specification in the spinal cord, as well as for the development of somatic motor neurons in the hindbrain. Cooperates with OLIG1 to establish the pMN domain of the embryonic neural tube. Antagonist of V2 interneuron and of NKX2-2-induced V3 interneuron development

By similarity.

Subunit structure: Interacts with NKX2-2

By similarity.

Subcellular location: Nucleus

By similarity. Cytoplasm

By similarity. Note: The NLS contained in the bHLH domain could be masked in the native form and translocation to the nucleus could be mediated by interaction either with class E bHLH partner protein or with NKX2-2

By similarity.

Tissue specificity: Expressed in the brain, in oligodendrocytes. Strongly expressed in oligodendrogliomas, while expression is weak to moderate in astrocytomas. Expression in glioblastomas highly variable. Ref.5 Ref.6

Induction: By SHH. Also induced by NKX6-1 in the developing spinal cord, but not in the rostral hindbrain

By similarity.

Domain: The bHLH is essential for interaction with NKX2-2

By similarity.

Involvement in disease: A chromosomal aberration involving OLIG2 may be a cause of a form of T-cell acute lymphoblastic leukemia (T-ALL). Translocation t(14;21)(q11.2;q22) with TCRA.

Sequence similarities: Contains 1 bHLH (basic helix-loop-helix) domain.

Sequence caution: The sequence AAC72247.1 differs from that shown. Reason: Erroneous initiation. The sequence AAF61215.1 differs from that shown. Reason: Erroneous initiation.