Rabbit MEK1 Polyclonal Antibody | anti-MEK1 antibody

MEK1, phosphorylated (Ser218/222), MEK2, Phosphorylated (S222/226) (MAPK/ERK Kinase 1, MEK 1, Dual Specificity Mitogen-activated Protein Kinase Kinase 1, MAP Kinase Kinase 1, MAP2K1, MAPKK 1, MKK1, PRKMK1, ERK Activator Kinase 1 / MAPK/ERK Kinase 2, Dual

Cat. Num. AAA6001123

• Gene Names: MEK1; ATMEK1; F20B18.180; F20B18_180; MAP kinase/ ERK kinase 1; MEK1; MITOGEN ACTIVATED PROTEIN KINASE KINASE; MITOGEN ACTIVATED PROTEIN KINASE KINASE 1; MKK1; NMAPKK
• Reactivity: Bovine, Canine, Chicken, Drosophila, Human, Mouse, Rat
• Applications: Western Blot
• Purity: Affinity Purified; Purified by immunoaffinity chromatography.
• Synonyms: MEK1; Polyclonal Antibody; phosphorylated (Ser218/222); MEK2; Phosphorylated (S222/226) (MAPK/ERK Kinase 1; MEK 1; Dual Specificity Mitogen-activated Protein Kinase Kinase 1; MAP Kinase Kinase 1; MAP2K1; MAPKK 1; MKK1; PRKMK1; ERK Activator Kinase 1 / MAPK/ERK Kinase 2; Dual; Anti -MEK1; anti-MEK1 antibody

• Host: Rabbit
• Reactivity: Bovine, Canine, Chicken, Drosophila, Human, Mouse, Rat
• Clonality: Polyclonal
• Isotype: IgG
• Specificity: Recognizes human MEK1, phosphorylated at S218/222 and MEK2, phosphorylated at S222/226. Species Crossreactivity: rat, mouse, drosophila, canine, bovine, chimpanzee, chicken.
• Purity/Purification: Affinity Purified; Purified by immunoaffinity chromatography.
• Form/Format: Supplied as a liquid in PBS, 0.05% BSA, 0.05% sodium azide.

• Applicable Applications for anti-MEK1 antibody: Western Blot (WB)
• Application Notes: Suitable for use in Western Blot.; Dilution: Western Blot: 2-5ug/ml
• Immunogen: Synthetic peptide corresponding to aa200-250, containing phosphorylated serine residues at positions 218 and 222, of human MEK1.
• Preparation and Storage: May be stored at 4 degree C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20 degree C. Aliquots are stable for at least 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.

Western Blot (WB)

(Western blot analysis of MEK1/2 phosphorylation in A375 whole cell lysate. Lane A shows phosphorylated MEK1/2 (upper band), and the weakly detected nonphosphorylated form (lower band), using MBS6001123 at 3ug/ml. Lane B shows the nonphosphorylated form of MEK1/2 at 5ug/ml.)

Related Product Information for anti-MEK1 antibody

MEK1 and MEK2 are integral components of the MAP kinase cascade that regulates cell growth and differentiation and this pathway also plays a key role in synaptic plasticity in brain. Activation of MEK1/2 occurs via phosphorylation of two serine residues (Ser218 and Ser222). Activated MEK1/2 then acts as a dual specificity kinase phosphorylating both a threonine and a tyrosine residue on ERK. This phosphorylation of ERK by MEK1/2 is a critical step in the MAP kinase cascade.

Product Categories/Family for anti-MEK1 antibody

Antibodies; Abs to Protein Kinases

NCBI and Uniprot Product Information

• NCBI GI #: 2196704
• NCBI GeneID: 828713
• UniProt Accession #: Q94A06; O04440; O04661
• Molecular Weight: 39,210 Da
• NCBI Official Full Name: MEK1
• NCBI Official Symbol: MEK1
• NCBI Official Synonym Symbols: ATMEK1; F20B18.180; F20B18_180; MAP kinase/ ERK kinase 1; MEK1; MITOGEN ACTIVATED PROTEIN KINASE KINASE; MITOGEN ACTIVATED PROTEIN KINASE KINASE 1; MKK1; NMAPKK
• NCBI Protein Information: mitogen-activated protein kinase kinase 1
• UniProt Protein Name: Mitogen-activated protein kinase kinase 1
• Protein Family: Meiosis-specific serine/threonine-protein kinase
• UniProt Gene Name: MKK1
• UniProt Synonym Gene Names: MEK1; MAP kinase kinase 1
• UniProt Entry Name: M2K1_ARATH

NCBI Description

Member of MAP Kinase Kinase. Likely functions in a stress-activated MAPK pathway. Can phosphorylate the MAPK AtMPK4, in response to stress. Gets phosphorylated by MEKK1 in response to wounding.

Uniprot Description

Function: May be involved in the cold and salinity stress-mediated MAP kinase signaling cascade (MEKK1, MEK1/MKK2 and MPK4/MPK6). Phosphorylates MPK4 in vitro. Ref.8

Catalytic activity: ATP + a protein = ADP + a phosphoprotein.

Enzyme regulation: Activated through serine and threonine phosphorylation in response to wounding, cold, drought, salt stresses, hydrogen peroxide, bacterial flagellin and laminarin beta-glucan. Ref.10 Ref.12

Subunit structure: Interacts with MEKK1 (MAPKKK) and MPK4. May form a ternary complex with. Ref.7

Tissue specificity: Expressed in roots, stem, flowers and siliques. Ref.1

Induction: By wounding. Ref.1 Ref.10 Ref.12

Post-translational modification: Phosphorylated on Thr-218 and Ser-224, which activates the enzyme. Ref.9 Ref.10

Sequence similarities: Belongs to the protein kinase superfamily. STE Ser/Thr protein kinase family. MAP kinase kinase subfamily.Contains 1 protein kinase domain.