Rabbit Mek1 Polyclonal Antibody | anti-MEK1 antibody

Mek1, NT (MAPK/ERK Kinase 1, MEK 1, Dual Specificity Mitogen-activated Protein Kinase Kinase 1, MAP Kinase Kinase 1, Map2k1, MAPKK 1, Prkmk1, ERK Activator Kinase 1)

Cat. Num. AAA647196

• Gene Names: MEK1; ATMEK1; F20B18.180; F20B18_180; MAP kinase/ ERK kinase 1; MEK1; MITOGEN ACTIVATED PROTEIN KINASE KINASE; MITOGEN ACTIVATED PROTEIN KINASE KINASE 1; MKK1; NMAPKK
• Reactivity: Human, Mouse, Rat, Xenopus
• Applications: ELISA, Western Blot, Immunoprecipitation, Immunohistochemistry
• Purity: Affinity Purified; Purified by Protein A affinity chromatography.
• Synonyms: Mek1; Polyclonal Antibody; NT (MAPK/ERK Kinase 1; MEK 1; Dual Specificity Mitogen-activated Protein Kinase Kinase 1; MAP Kinase Kinase 1; Map2k1; MAPKK 1; Prkmk1; ERK Activator Kinase 1); Anti -Mek1; anti-MEK1 antibody

• Host: Rabbit
• Reactivity: Human, Mouse, Rat, Xenopus
• Clonality: Polyclonal
• Isotype: IgG
• Specificity: Detects an ~48kD protein, corresponding to the apparent molecular mass of Mek1 in rat, human, mouse, and Xenopus. This ~48kD band can be specifically inhibited by the relevant peptide. Spercies Sequence Homology: Hamster and rabbit.
• Purity/Purification: Affinity Purified; Purified by Protein A affinity chromatography.
• Form/Format: Supplied as a liquid in PBS, pH 7.2, 0.09% sodium azide and 50% glycerol.

• Applicable Applications for anti-MEK1 antibody: ELISA (EL/EIA), Western Blot (WB), Immunoprecipitation (IP), Immunohistochemistry (IHC)
• Application Notes: Dilution: Western Blot (Colorimetric): 1ug/ml
• Immunogen: Synthetic peptide aa1-12, MPKKKPTPIQLN(C) corresponding to rat Mek1(KLH.)
• Positive Control: H1838-01 HeLa Cell Lysate, Heat Shocked
• Preparation and Storage: May be stored at 4 degree C for short-term only. For long-term storage, aliquot and store at -20 degree C. Aliquots are stable for at least 12 months at -20 degree C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.

Western Blot (WB)

(Western Blot analysis using MBS647196: Lane 1: MW marker Lane 2: HeLa (heat shocked) Lane 3: Rat spleen extract Lane 4: Mouse brain extract.)

Immunohistochemistry (IHC)

(Immunohistochemistry analysis of human breast cancer tissue using MBS647196.)

Immunofluorescence (IF)

(Immunofluorescent analysis (confocal) of HeLa cells using MBS647196 (green); nuclei are stained in blue pseudocolor using DRAQ5.)

Related Product Information for anti-MEK1 antibody

MAP kinase kinase (Mek1), also known as mitogen activated protein kinase kinase, MAP kinase kinase, ERK kinase 1 or microtubule-associated protein 2 kinase, is a 43 kD dual-specificity protein kinase that phosphorylates threonine and tyrosine residues on MAP kinases such as Erk 1 and 2 (1). Phosphorylation by Mek1 increases Erksí ability to phosphorylate micrtubule-associated proteins and myelin basic proteins. Mek1 is involved in diverse arrays of cellular processes such as stress-activated response, apoptosis (2), cytokine-induced cell proliferation (3), and DNA recombination during meiosis (4). Mek1 is activated by phosphorylation on serine 218 and 222 by serine-threonine kinase, Raf-1, which is part of the p21ras signal transduction pathway (5).

Product Categories/Family for anti-MEK1 antibody

Antibodies; Abs to Protein Kinases

NCBI and Uniprot Product Information

• NCBI GI #: 2196704
• NCBI GeneID: 828713
• UniProt Accession #: Q94A06; O04440; O04661
• Molecular Weight: 39,210 Da
• NCBI Official Full Name: MEK1
• NCBI Official Symbol: MEK1
• NCBI Official Synonym Symbols: ATMEK1; F20B18.180; F20B18_180; MAP kinase/ ERK kinase 1; MEK1; MITOGEN ACTIVATED PROTEIN KINASE KINASE; MITOGEN ACTIVATED PROTEIN KINASE KINASE 1; MKK1; NMAPKK
• NCBI Protein Information: mitogen-activated protein kinase kinase 1
• UniProt Protein Name: Mitogen-activated protein kinase kinase 1
• Protein Family: Meiosis-specific serine/threonine-protein kinase
• UniProt Gene Name: MKK1
• UniProt Synonym Gene Names: MEK1; MAP kinase kinase 1
• UniProt Entry Name: M2K1_ARATH

NCBI Description

Member of MAP Kinase Kinase. Likely functions in a stress-activated MAPK pathway. Can phosphorylate the MAPK AtMPK4, in response to stress. Gets phosphorylated by MEKK1 in response to wounding.

Uniprot Description

Function: May be involved in the cold and salinity stress-mediated MAP kinase signaling cascade (MEKK1, MEK1/MKK2 and MPK4/MPK6). Phosphorylates MPK4 in vitro. Ref.8

Catalytic activity: ATP + a protein = ADP + a phosphoprotein.

Enzyme regulation: Activated through serine and threonine phosphorylation in response to wounding, cold, drought, salt stresses, hydrogen peroxide, bacterial flagellin and laminarin beta-glucan. Ref.10 Ref.12

Subunit structure: Interacts with MEKK1 (MAPKKK) and MPK4. May form a ternary complex with. Ref.7

Tissue specificity: Expressed in roots, stem, flowers and siliques. Ref.1

Induction: By wounding. Ref.1 Ref.10 Ref.12

Post-translational modification: Phosphorylated on Thr-218 and Ser-224, which activates the enzyme. Ref.9 Ref.10

Sequence similarities: Belongs to the protein kinase superfamily. STE Ser/Thr protein kinase family. MAP kinase kinase subfamily.Contains 1 protein kinase domain.