Rabbit anti-Human MEK1 Polyclonal Antibody | anti-MEK1 antibody

MEK1, phosphorylated (Thr291) (MAPK/ERK Kinase 1, MEK 1, Dual Specificity Mitogen-Activated Protein Kinase Kinase 1, MAP Kinase Kinase 1, MAP2K1, MAPKK 1, MKK1, PRKMK1, ERK Activator Kinase 1)

Cat. Num. AAA626535

• Gene Names: MEK1; ATMEK1; F20B18.180; F20B18_180; MAP kinase/ ERK kinase 1; MEK1; MITOGEN ACTIVATED PROTEIN KINASE KINASE; MITOGEN ACTIVATED PROTEIN KINASE KINASE 1; MKK1; NMAPKK
• Reactivity: Human
• Applications: ELISA, Western Blot
• Purity: Affinity Purified; Purified by immunoaffinity chromatography.
• Synonyms: MEK1; Polyclonal Antibody; phosphorylated (Thr291) (MAPK/ERK Kinase 1; MEK 1; Dual Specificity Mitogen-Activated Protein Kinase Kinase 1; MAP Kinase Kinase 1; MAP2K1; MAPKK 1; MKK1; PRKMK1; ERK Activator Kinase 1); Anti -MEK1; anti-MEK1 antibody

• Host: Rabbit
• Reactivity: Human
• Clonality: Polyclonal
• Isotype: IgG
• Specificity: Detects endogenous levels of MEK1 only when phosphorylated at threonine 291.
• Purity/Purification: Affinity Purified; Purified by immunoaffinity chromatography.
• Form/Format: Supplied as a liquid in PBS (without Mg2+ and Ca2+), pH 7.4, 150mM sodium chloride, 0.02% sodium azide and 50% glycerol.

• Applicable Applications for anti-MEK1 antibody: ELISA (EL/EIA), Western Blot (WB)
• Application Notes: Suitable for use in ELISA and Western Blot.; Dilution: Western Blot: 1:500-1:1000
• Immunogen: Synthesized phosphopeptide derived from human MEK1 around the phosphorylation site of threonine 291 (P-R-TP-P-G)
• Preparation and Storage: For long-term storage, aliquot and store at -20 degree C. Aliquots are stable for at least 12 months at -20 degree C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.

Western Blot (WB)

(Western Blot analysis od extracts from 293 cells untreated or treated with 10% serum)

Product Categories/Family for anti-MEK1 antibody

Antibodies; Abs to Protein Kinases

NCBI and Uniprot Product Information

• NCBI GI #: 2196704
• NCBI GeneID: 828713
• UniProt Accession #: Q94A06; O04440; O04661
• Molecular Weight: 39,210 Da
• NCBI Official Full Name: MEK1
• NCBI Official Symbol: MEK1
• NCBI Official Synonym Symbols: ATMEK1; F20B18.180; F20B18_180; MAP kinase/ ERK kinase 1; MEK1; MITOGEN ACTIVATED PROTEIN KINASE KINASE; MITOGEN ACTIVATED PROTEIN KINASE KINASE 1; MKK1; NMAPKK
• NCBI Protein Information: mitogen-activated protein kinase kinase 1
• UniProt Protein Name: Mitogen-activated protein kinase kinase 1
• Protein Family: Meiosis-specific serine/threonine-protein kinase
• UniProt Gene Name: MKK1
• UniProt Synonym Gene Names: MEK1; MAP kinase kinase 1
• UniProt Entry Name: M2K1_ARATH

NCBI Description

Member of MAP Kinase Kinase. Likely functions in a stress-activated MAPK pathway. Can phosphorylate the MAPK AtMPK4, in response to stress. Gets phosphorylated by MEKK1 in response to wounding.

Uniprot Description

Function: May be involved in the cold and salinity stress-mediated MAP kinase signaling cascade (MEKK1, MEK1/MKK2 and MPK4/MPK6). Phosphorylates MPK4 in vitro. Ref.8

Catalytic activity: ATP + a protein = ADP + a phosphoprotein.

Enzyme regulation: Activated through serine and threonine phosphorylation in response to wounding, cold, drought, salt stresses, hydrogen peroxide, bacterial flagellin and laminarin beta-glucan. Ref.10 Ref.12

Subunit structure: Interacts with MEKK1 (MAPKKK) and MPK4. May form a ternary complex with. Ref.7

Tissue specificity: Expressed in roots, stem, flowers and siliques. Ref.1

Induction: By wounding. Ref.1 Ref.10 Ref.12

Post-translational modification: Phosphorylated on Thr-218 and Ser-224, which activates the enzyme. Ref.9 Ref.10

Sequence similarities: Belongs to the protein kinase superfamily. STE Ser/Thr protein kinase family. MAP kinase kinase subfamily.Contains 1 protein kinase domain.