Rabbit MEK1 Polyclonal Antibody | anti-MEK1 antibody

MEK1, ID (MAP2K1, MEK1, PRKMK1, ERK activator kinase 1, MAPK/ERK kinase 1)

Cat. Num. AAA6000390

• Gene Names: MEK1; ATMEK1; F20B18.180; F20B18_180; MAP kinase/ ERK kinase 1; MEK1; MITOGEN ACTIVATED PROTEIN KINASE KINASE; MITOGEN ACTIVATED PROTEIN KINASE KINASE 1; MKK1; NMAPKK
• Reactivity: Human, Mouse, Rat
• Applications: ELISA, Western Blot, Immunohistochemistry
• Purity: Affinity Purified; Purified by immunoaffinity chromatography
• Synonyms: MEK1; Polyclonal Antibody; ID (MAP2K1; PRKMK1; ERK activator kinase 1; MAPK/ERK kinase 1); Anti -MEK1; anti-MEK1 antibody

• Host: Rabbit
• Reactivity: Human, Mouse, Rat
• Clonality: Polyclonal
• Isotype: IgG
• Specificity: Recognizes human MAP2K1/MEK1. Species Crossreactivity: Mouse, rat.
• Purity/Purification: Affinity Purified; Purified by immunoaffinity chromatography
• Form/Format: Supplied as a liquid in PBS, (without Mg2+, Ca2+), pH 7.4, 150mM sodium chloride, 0.02% sodium azide, 50% glycerol.

• Applicable Applications for anti-MEK1 antibody: ELISA (EL/EIA), Western Blot (WB), Immunohistochemistry (IHC)
• Application Notes: Suitable for use in ELISA, Western Blot, Immunohistochemistry.; Dilution: ELISA: 1:1000; Western Blot: 1:500-1:1000; Immunohistochemistry (Paraffin): 10ug/ml
• Immunogen: Synthetic peptide corresponding to human MAP2K1/MEK1.
• Preparation and Storage: May be stored at 4 degree C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20 degree C. Aliquots are stable for at least 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.

Immunohistochemistry (IHC)

(Human Adrenal: Formalin-Fixed, Paraffin-Embedded (FFPE))

Western Blot (WB)

(Western blot analysis of extracts from HuvEc cells, using MEK1. The lane on the right is treated with the synthesized peptide.)

Related Product Information for anti-MEK1 antibody

MEK1, a MAP2K type protein kinase, is a member of the Ras MAPK pathway. This pathway modulates the activity of transcription factors, structural proteins and other protein kinases, leading to biological responses such as cell proliferation and differentiation. MEK1 contains a proline-rich segment that is recognized by the scaffold protein MEK binding partner-1 (MP1). MP1 associates with non-phosphorylated MEK1, forming a complex which associates with and phosphorylates MAP kinases ERK1 and 2. Additionally, MEK1 signaling is reported to have a strong negative effect on myogenic differentiation antigen (MYOD) activity via a mechanism involving the binding of MEK1 to the nuclear MYOD transcriptional complex. MEK1 is phosphorylated by several kinases, including Raf1, c-Mos and MEKK1. PAK1 also phosphorylates MEK1 resulting in enhancement of MEK's interaction with Raf-1. Constitutive activation of MEK1 results in cellular transformation; thus, it is a likely target for pharmacologic intervention in proliferative disease. MEK inhibitors have been studied for clinical management of colon cancer and inhibition of viral replication in host cells. MEK1 is a substrate for the anthrax lethal factor (LF).

Product Categories/Family for anti-MEK1 antibody

Antibodies; Abs to Protein Kinases

NCBI and Uniprot Product Information

• NCBI GI #: 2196704
• NCBI GeneID: 828713
• UniProt Accession #: Q94A06; O04440; O04661
• Molecular Weight: 39,210 Da
• NCBI Official Full Name: MEK1
• NCBI Official Symbol: MEK1
• NCBI Official Synonym Symbols: ATMEK1; F20B18.180; F20B18_180; MAP kinase/ ERK kinase 1; MEK1; MITOGEN ACTIVATED PROTEIN KINASE KINASE; MITOGEN ACTIVATED PROTEIN KINASE KINASE 1; MKK1; NMAPKK
• NCBI Protein Information: mitogen-activated protein kinase kinase 1
• UniProt Protein Name: Mitogen-activated protein kinase kinase 1
• Protein Family: Meiosis-specific serine/threonine-protein kinase
• UniProt Gene Name: MKK1
• UniProt Synonym Gene Names: MEK1; MAP kinase kinase 1
• UniProt Entry Name: M2K1_ARATH

NCBI Description

Member of MAP Kinase Kinase. Likely functions in a stress-activated MAPK pathway. Can phosphorylate the MAPK AtMPK4, in response to stress. Gets phosphorylated by MEKK1 in response to wounding.

Uniprot Description

Function: May be involved in the cold and salinity stress-mediated MAP kinase signaling cascade (MEKK1, MEK1/MKK2 and MPK4/MPK6). Phosphorylates MPK4 in vitro. Ref.8

Catalytic activity: ATP + a protein = ADP + a phosphoprotein.

Enzyme regulation: Activated through serine and threonine phosphorylation in response to wounding, cold, drought, salt stresses, hydrogen peroxide, bacterial flagellin and laminarin beta-glucan. Ref.10 Ref.12

Subunit structure: Interacts with MEKK1 (MAPKKK) and MPK4. May form a ternary complex with. Ref.7

Tissue specificity: Expressed in roots, stem, flowers and siliques. Ref.1

Induction: By wounding. Ref.1 Ref.10 Ref.12

Post-translational modification: Phosphorylated on Thr-218 and Ser-224, which activates the enzyme. Ref.9 Ref.10

Sequence similarities: Belongs to the protein kinase superfamily. STE Ser/Thr protein kinase family. MAP kinase kinase subfamily.Contains 1 protein kinase domain.