Western Blot (WB)
(Ki-67 antibody tested by Western blot. Detection of Ki-67 by Western blot. Raji nuclear extract (30 ?g) probed with Ki-67 antibody (1:500 dilution). Lane 2: pre-incubation of antibody with immunizing peptide.)
Rabbit anti-Human Ki-67 Polyclonal Antibody | anti-Ki-67 antibody
Ki-67 antibody (pAb)
Gene Names
MKI67; KIA
Reactivity
Human
Applications
Western Blot
Purity
Affinity Purified
Synonyms
Ki-67; Polyclonal Antibody; Ki-67 antibody (pAb); MKI67; KIA; MIB-1; anti-Ki-67 antibody
Host
Rabbit
Reactivity
Human
Clonality
Polyclonal
Isotype
IgG
Purity/Purification
Affinity Purified
Form/Format
Purified IgG in 70mM Tris (pH8), 105mM NaCl, 31mM glycine, 0.07mM EDTA, 30% glycerol and 0.035% sodium azide. Sodium azide is highly toxic.
Applicable Applications for anti-Ki-67 antibody
Western Blot (WB)
Application Notes
Applications Validated:
WB: 1:500 1:1,000 dilution
Note: Many chromatin-bound proteins are not soluble in a low salt nuclear extract and fractionate to the pellet. Therefore, we recommend a High Salt/Sonication Protocol when preparing nuclear extracts for Western blot.
WB: 1:500 1:1,000 dilution
Note: Many chromatin-bound proteins are not soluble in a low salt nuclear extract and fractionate to the pellet. Therefore, we recommend a High Salt/Sonication Protocol when preparing nuclear extracts for Western blot.
Immunogen
This Ki-67 antibody was raised against a peptide within the N-terminal region of human Ki-67.
Preparation and Storage
Some products may be shipped at room temperature. This will not affect their stability or performance. Avoid repeated freeze/thaw cycles by aliquoting items into single-use fractions for storage at -20 degree C for up to 2 years. Keep all reagents on ice when not in storage.1ug/ul
Western Blot (WB)
(Ki-67 antibody tested by Western blot. Detection of Ki-67 by Western blot. Raji nuclear extract (30 ?g) probed with Ki-67 antibody (1:500 dilution). Lane 2: pre-incubation of antibody with immunizing peptide.)
Western Blot (WB)
(Ki-67 antibody tested by Western blot. Detection of Ki-67 by Western blot. Raji nuclear extract (30 ?g) probed with Ki-67 antibody (1:500 dilution). Lane 2: pre-incubation of antibody with immunizing peptide.)
Related Product Information for anti-Ki-67 antibody
Short Description: Our Ki-67 antibody (pAb) was raised in a Rabbit host. It has been validated for use in Western blot; it has been shown to react with Human samples.
Background: Ki-67 encodes a protein that is highly expressed in dividing cells but not in quiescent cells. Ki-67 is a useful marker for rapidly dividing cells and has been used as a marker for cancer cells, Including breast, bladder, brain, colon, and prostate cancer.
Background: Ki-67 encodes a protein that is highly expressed in dividing cells but not in quiescent cells. Ki-67 is a useful marker for rapidly dividing cells and has been used as a marker for cancer cells, Including breast, bladder, brain, colon, and prostate cancer.
Product Categories/Family for anti-Ki-67 antibody
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
Molecular Weight
358,694 Da
NCBI Official Full Name
antigen KI-67 isoform 1
NCBI Official Synonym Full Names
antigen identified by monoclonal antibody Ki-67
NCBI Official Symbol
MKI67
NCBI Official Synonym Symbols
KIA
NCBI Protein Information
antigen KI-67; proliferation-related Ki-67 antigen
UniProt Protein Name
Antigen KI-67
UniProt Gene Name
MKI67
UniProt Entry Name
KI67_HUMAN
Uniprot Description
KI-67: a protein that may be a marker of proliferating cells, involved in chromatin compaction. Its expression is altered in many tumor types including osteosarcomas, histiocytomas, prostate, breast and esophageal cancers. Mutated in colon, cervical and lung cancers.
Protein type: Nucleolus; Cell cycle regulation
Chromosomal Location of Human Ortholog: 10q26.2
Cellular Component: membrane; cytoplasm; nucleolus; condensed chromosome; nucleus; chromosome, pericentric region
Molecular Function: protein C-terminus binding; protein binding; ATP binding
Biological Process: cell proliferation; meiosis
Similar Products
Product Notes
The Ki-67 mki67 (Catalog #AAA388581) is an Antibody produced from Rabbit and is intended for research purposes only. The product is available for immediate purchase. The Ki-67 antibody (pAb) reacts with Human and may cross-react with other species as described in the data sheet. AAA Biotech's Ki-67 can be used in a range of immunoassay formats including, but not limited to, Western Blot (WB). Applications Validated: WB: 1:500 1:1,000 dilution Note: Many chromatin-bound proteins are not soluble in a low salt nuclear extract and fractionate to the pellet. Therefore, we recommend a High Salt/Sonication Protocol when preparing nuclear extracts for Western blot. Researchers should empirically determine the suitability of the Ki-67 mki67 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "Ki-67, Polyclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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