Typical Standard Curve/Testing Data
Canine Antigen KI-67 (Ki-67) ELISA Kit | MKI67 elisa kit
Canine Antigen KI-67 (Ki-67) ELISA Kit
Typical Standard Curve/Testing Data
Typical Standard Curve/Testing Data
Principle of the Assay: Ki-67 ELISA kit applies the competitive enzyme immunoassay technique utilizing a monoclonal anti-Ki-67 antibody and an Ki-67-HRP conjugate. The assay sample and buffer are incubated together with Ki-67-HRP conjugate in pre-coated plate for one hour. After the incubation period, the wells are decanted and washed five times. The wells are then incubated with a substrate for HRP enzyme. The product of the enzyme-substrate reaction forms a blue colored complex. Finally, a stop solution is added to stop the reaction, which will then turn the solution yellow. The intensity of color is measured spectrophotometrically at 450nm in a microplate reader. The intensity of the color is inversely proportional to the Ki-67 concentration since Ki-67 from samples and Ki-67-HRP conjugate compete for the anti-Ki-67 antibody binding site. Since the number of sites is limited, as more sites are occupied by Ki-67 from the sample, fewer sites are left to bind Ki-67-HRP conjugate. A standard curve is plotted relating the intensity of the color (O.D.) to the concentration of standards. The Ki-67 concentration in each sample is interpolated from this standard curve.
NCBI and Uniprot Product Information
NCBI Description
This gene encodes a nuclear protein that is associated with and may be necessary for cellular proliferation. Alternatively spliced transcript variants have been described. A related pseudogene exists on chromosome X. [provided by RefSeq, Mar 2009]
Uniprot Description
KI-67: a protein that may be a marker of proliferating cells, involved in chromatin compaction. Its expression is altered in many tumor types including osteosarcomas, histiocytomas, prostate, breast and esophageal cancers. Mutated in colon, cervical and lung cancers.
Protein type: Cell cycle regulation; Nucleolus
Chromosomal Location of Human Ortholog: 10q26.2
Cellular Component: membrane; cytoplasm; nucleolus; condensed chromosome; nucleus; chromosome, pericentric region
Molecular Function: protein C-terminus binding; protein binding; ATP binding
Biological Process: meiosis; cell proliferation
Research Articles on MKI67
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Product Notes
The Canine MKI67 mki67 (Catalog #AAA7212587) is an ELISA Kit and is intended for research purposes only. The product is available for immediate purchase. The AAA7212587 ELISA Kit recognizes Canine MKI67. It is sometimes possible for the material contained within the vial of "Antigen KI-67 (Ki-67), ELISA Kit" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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