Human Antigen KI 67 (Ki 67) ELISA Kit | MKI67 elisa kit

Human Antigen KI 67 (Ki 67) ELISA Kit

Cat. Num. AAA7243209

• Gene Names: MKI67; KIA; MIB-; MIB-1; PPP1R105
• Reactivity: Human
• Synonyms: Antigen KI 67 (Ki 67); Human Antigen KI 67 (Ki 67) ELISA Kit; MKI67 elisa kit

• Reactivity: Human

• Samples: Serum, plasma, Cell Culture Supernatants, body fluid and tissue homogenate
• Assay Type: Competitive
• Sensitivity: 0.1 ng/mL.
• Intended Uses: This Ki 67 ELISA kit is a 1.5 hour solid-phase ELISA designed for the quantitative determination of Human Ki 67. This ELISA kit for research use only, not for therapeutic applications!
• Preparation and Storage: Store all reagents at 2-8 degree C.

Typical Testing Data/Standard Curve (for reference only)

Related Product Information for MKI67 elisa kit

Principle of the assay: Ki 67 ELISA kit applies the competitive enzyme immunoassay technique utilizing a monoclonal anti-Ki 67 antibody and an Ki 67-HRP conjugate. The assay sample and buffer are incubated together with Ki 67-HRP conjugate in pre-coated plate for one hour. After the incubation period, the wells are decanted and washed five times. The wells are then incubated with a substrate for HRP enzyme. The product of the enzyme-substrate reaction forms a blue colored complex. Finally, a stop solution is added to stop the reaction, which will then turn the solution yellow. The intensity of color is measured spectrophotometrically at 450nm in a microplate reader. The intensity of the color is inversely proportional to the Ki 67 concentration since Ki 67 from samples and Ki 67-HRP conjugate compete for the anti-Ki 67 antibody binding site. Since the number of sites is limited, as more sites are occupied by Ki 67 from the sample, fewer sites are left to bind Ki 67-HRP conjugate. A standard curve is plotted relating the intensity of the color (O.D.) to the concentration of standards. The Ki 67 concentration in each sample is interpolated from this standard curve.

Product Categories/Family for MKI67 elisa kit

Cancer

NCBI and Uniprot Product Information

• NCBI GI #: 118572663
• NCBI GeneID: 4288
• Molecular Weight: 319,444 Da
• NCBI Official Full Name: Antigen KI-67
• NCBI Official Synonym Full Names: marker of proliferation Ki-67
• NCBI Official Symbol: MKI67
• NCBI Official Synonym Symbols: KIA; MIB-; MIB-1; PPP1R105
• NCBI Protein Information: antigen KI-67; proliferation-related Ki-67 antigen; protein phosphatase 1, regulatory subunit 105; antigen identified by monoclonal antibody Ki-67
• UniProt Protein Name: Antigen KI-67
• Protein Family: Proliferation marker protein
• UniProt Gene Name: MKI67
• UniProt Entry Name: KI67_HUMAN

NCBI Description

This gene encodes a nuclear protein that is associated with and may be necessary for cellular proliferation. Alternatively spliced transcript variants have been described. A related pseudogene exists on chromosome X. [provided by RefSeq, Mar 2009]

Uniprot Description

KI-67: a protein that may be a marker of proliferating cells, involved in chromatin compaction. Its expression is altered in many tumor types including osteosarcomas, histiocytomas, prostate, breast and esophageal cancers. Mutated in colon, cervical and lung cancers.

Protein type: Nucleolus; Cell cycle regulation

Chromosomal Location of Human Ortholog: 10q26.2

Cellular Component: membrane; cytoplasm; nucleolus; condensed chromosome; nucleus; chromosome, pericentric region

Molecular Function: protein C-terminus binding; protein binding; ATP binding

Biological Process: meiosis; cell proliferation