Typical Testing Data/Standard Curve (for reference only)
PPBP/CXCL7 elisa kit
Mouse PPBP/CXCL7 PicoKine ELISA Kit
Gene Names
Ppbp; TGB; LDGF; MDGF; TGB1; CTAP3; Cxcl7; NAP-2; Scyb7; b-TG1; LA-PF4; THBGB1; CTAPIII; beta-TG; AI854500; NAP-2-L1; 2400003M24Rik
Reactivity
MouseThere is no detectable cross-reactivity with other relevant proteins.
Applications
ELISA
Synonyms
PPBP/CXCL7; Mouse PPBP/CXCL7 PicoKine ELISA Kit; Chemokine (C-X-C motif) ligand 7; isoform CRA_b; PPBP/CXCL7 elisa kit
Reactivity
Mouse
There is no detectable cross-reactivity with other relevant proteins.
There is no detectable cross-reactivity with other relevant proteins.
Specificity
Natural and recombinant Mouse PPBP/CXCL7
Sequence Length
113
Applicable Applications for PPBP/CXCL7 elisa kit
ELISA (EIA)
Assay Type
Quantitative Sandwich
Detection Range
15.6pg/ml-1000pg/ml
Sensitivity
<2pg/ml
Intra-assay Precision
Intra-Assay Precision (Precision within an assay) Three samples of known concentration were tested on one plate to assess intra-assay precision
Inter-assay Precision
Inter-Assay Precision (Precision between assays) Three samples of known concentration were tested in separate assays to assess inter-assay precision.
Preparation and Storage
Store at 4 degree C for 6 months, at -20 degree C for 12 months. Avoid multiple freeze-thaw cycles.
Typical Testing Data/Standard Curve (for reference only)
Typical Testing Data/Standard Curve (for reference only)
Related Product Information for PPBP/CXCL7 elisa kit
Principle of the Assay: Mouse PPBP/CXCL7 ELISA Kit was based on standard sandwich enzyme-linked immune- sorbent assay technology. A monoclonal antibody from rat specific for PPBP/CXCL7 has been precoated onto 96-well plates. Standards(Expression system for standard: E.coli, Immunogen sequence: K40-Y113) and test samples are added to the wells, a biotinylated detection biotinylated polyclonal antibody from goat specific for PPBP/CXCL7 is added subsequently and then followed by washing with PBS or TBS buffer. Avidin-Biotin-Peroxidase Complex was added and unbound conjugates were washed away with PBS or TBS buffer. HRP substrate TMB was used to visualize HRP enzymatic reaction. TMB was catalyzed by HRP to produce a blue color product that changed into yellow after adding acidic stop solution. The density of yellow is proportional to the Mouse PPBP/CXCL7 amount of sample captured in plate.
Background: Chemokine (C-X-C motif) ligand 7 (CXCL7), also called PPBP or SCYB7, is a human gene. The encoded protein, Chemokine (C-X-C motif) ligand is a small cytokine belonging to the CXC chemokine family. CXCL7 is mapped to 4q13.3. It is a protein that is released in large amounts from platelets following their activation. CXCL7 is the precursor of the 2 platelet alpha-granule proteins, platelet basic protein (PBP) and connective tissue-activating peptide III (CTAP3). It stimulates various processes including mitogenesis, synthesis of extracellular matrix, glucose metabolism and synthesis of plasminogen activator. It also stimulates the formation and secretion of plasminogen activator by synovial cells.
Background: Chemokine (C-X-C motif) ligand 7 (CXCL7), also called PPBP or SCYB7, is a human gene. The encoded protein, Chemokine (C-X-C motif) ligand is a small cytokine belonging to the CXC chemokine family. CXCL7 is mapped to 4q13.3. It is a protein that is released in large amounts from platelets following their activation. CXCL7 is the precursor of the 2 platelet alpha-granule proteins, platelet basic protein (PBP) and connective tissue-activating peptide III (CTAP3). It stimulates various processes including mitogenesis, synthesis of extracellular matrix, glucose metabolism and synthesis of plasminogen activator. It also stimulates the formation and secretion of plasminogen activator by synovial cells.
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
UniProt Accession #
Molecular Weight
12,252 Da
NCBI Official Full Name
platelet basic protein
NCBI Official Synonym Full Names
pro-platelet basic protein
NCBI Official Symbol
Ppbp
NCBI Official Synonym Symbols
TGB; LDGF; MDGF; TGB1; CTAP3; Cxcl7; NAP-2; Scyb7; b-TG1; LA-PF4; THBGB1; CTAPIII; beta-TG; AI854500; NAP-2-L1; 2400003M24Rik
NCBI Protein Information
platelet basic protein
UniProt Protein Name
Chemokine (C-X-C motif) ligand 7, isoform CRA_b
UniProt Gene Name
Ppbp
Research Articles on PPBP/CXCL7
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Product Notes
The Mouse There is no detectable cross-reactivity with other relevant proteins. PPBP/CXCL7 ppbp (Catalog #AAA1751073) is an ELISA Kit and is intended for research purposes only. The product is available for immediate purchase. The AAA1751073 ELISA Kit recognizes Mouse There is no detectable cross-reactivity with other relevant proteins. PPBP/CXCL7. AAA Biotech's PPBP/CXCL7 can be used in a range of immunoassay formats including, but not limited to, ELISA (EIA). Researchers should empirically determine the suitability of the PPBP/CXCL7 ppbp for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "PPBP/CXCL7, ELISA Kit" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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