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Western Blot (WB) (Western Blot analysis of HeLa cells using NAPRT1 Monoclonal Antibody at dilution of 1:1500)

Mouse anti-Human, Mouse NAPRT Monoclonal Antibody | anti-NAPRT antibody

NAPRT Monoclonal Antibody

Gene Names
NAPRT; NAPRT1; PP3856
Reactivity
Human, Mouse
Applications
Western Blot, Immunohistochemistry, Immunofluorescence, ELISA
Purity
Protein A purification
Synonyms
NAPRT; Monoclonal Antibody; NAPRT Monoclonal Antibody; FHA HIT interacting protein; FHA-HIT-interacting protein; FHIP; NAPRT1; NAPRTase; Nicotinate phosphoribosyltransferase; Nicotinate phosphoribosyltransferase domain containing 1; Nicotinate phosphoribosyltransferase domain-containing protein 1; Nicotinic acid phosphoribosyltransferase; PNCB; PP3856; anti-NAPRT antibody
Ordering
For Research Use Only!
Host
Mouse
Reactivity
Human, Mouse
Clonality
Monoclonal
Isotype
IgG2a
Clone Number
77
Purity/Purification
Protein A purification
Concentration
1mg/mL (varies by lot)
Sequence Length
525
Applicable Applications for anti-NAPRT antibody
Western Blot (WB), Immunohistochemistry (IHC), Immunofluorescence (IF), ELISA (EIA)
Application Notes
WB: 1:500-1:5000
IHC: 1:20-1:200
IF: 1:50-1:500
Immunogen
Fusion protein of NAPRT1
Buffer
PBS with 0.02% sodium azide, 50% glycerol, PH7.3
Conjugation
Unconjugated
Preparation and Storage
Store at -20 degree C. Avoid freeze / thaw cycles.

Western Blot (WB)

(Western Blot analysis of HeLa cells using NAPRT1 Monoclonal Antibody at dilution of 1:1500)

Western Blot (WB) (Western Blot analysis of HeLa cells using NAPRT1 Monoclonal Antibody at dilution of 1:1500)
Related Product Information for anti-NAPRT antibody
Nicotinic acid (NA) is a coenzyme in cellular redox reactions, and is an essential component of metabolic pathways in all living cells. NAPRT1 (Nicotinate phosphoribosyltransferase) is essential for increasing cellular NAD levels and, thus, to prevent oxidative stress of cells. NAPRT1 converts Nicotinic acid (NA; niacin) to NA mononucleotide (NaMN), which is then converted to NA adenine dinucleotide (NaAD), and finally to nicotinamide adenine dinucleotide (NAD).
Product Categories/Family for anti-NAPRT antibody

NCBI and Uniprot Product Information

NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
UniProt Accession #
Molecular Weight
Calculated: 55kDa
Observed: 51kDa
NCBI Official Full Name
nicotinate phosphoribosyltransferase isoform 2
NCBI Official Synonym Full Names
nicotinate phosphoribosyltransferase
NCBI Official Symbol
NAPRT
NCBI Official Synonym Symbols
NAPRT1; PP3856
NCBI Protein Information
nicotinate phosphoribosyltransferase
UniProt Protein Name
Nicotinate phosphoribosyltransferase
UniProt Gene Name
NAPRT
UniProt Synonym Gene Names
FHIP; NAPRT1; NAPRTase

NCBI Description

Nicotinic acid (NA; niacin) is converted by nicotinic acid phosphoribosyltransferase (NAPRT; EC 2.4.2.11) to NA mononucleotide (NaMN), which is then converted to NA adenine dinucleotide (NaAD), and finally to nicotinamide adenine dinucleotide (NAD), which serves as a coenzyme in cellular redox reactions and is an essential component of a variety of processes in cellular metabolism including response to stress (Hara et al., 2007).[supplied by OMIM, Mar 2008]

Uniprot Description

Catalyzes the conversion of nicotinic acid (NA) to NA mononucleotide (NaMN). Essential for NA to increase cellular NAD levels and prevent oxidative stress of the cells.

Research Articles on NAPRT

Similar Products

Product Notes

The NAPRT naprt (Catalog #AAA2541992) is an Antibody produced from Mouse and is intended for research purposes only. The product is available for immediate purchase. The NAPRT Monoclonal Antibody reacts with Human, Mouse and may cross-react with other species as described in the data sheet. AAA Biotech's NAPRT can be used in a range of immunoassay formats including, but not limited to, Western Blot (WB), Immunohistochemistry (IHC), Immunofluorescence (IF), ELISA (EIA). WB: 1:500-1:5000 IHC: 1:20-1:200 IF: 1:50-1:500. Researchers should empirically determine the suitability of the NAPRT naprt for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "NAPRT, Monoclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.

Precautions

All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.

Disclaimer

Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.

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