SDS-Page
(Peptide Competition Extracts prepared from CEFs (1) or CEFs expressing Pyk2 and plated on fibronectin (2-7) were resolved by SDS-PAGE on a 10% polyacrylamide gel and transferred to PVDF. Membranes were blocked with a 5% BSA-TBST buffer overnight at 4oC, then were incubated with Pyk2 [pY580] antibody for two hours at room temperature in a 1% BSA-TBST buffer, following prior incubation with: no peptide (1, 2), the non-phosphopeptide corresponding to the immunogen (3), the phosphopeptide corresponding to Pyk2 [pY579] (4), a phosphopeptide derived from the corresponding region of FAK (5), a generic phosphotyrosine-containing peptide (6) or, the phosphopeptide immunogen (7). After washing, membranes were incubated with goat F(ab’)2 anti-rabbit IgG HRP conjugate and bands were detected using the Pierce SuperSignal‘ method. The data show that only the peptide corresponding to Pyk2 [pY580] completely blocks the antibody signal, thereby demonstrating the specificity of the antibody.)
Rabbit anti-Human Pyk2 Polyclonal Antibody | anti-PTK2B antibody
Pyk2, phosphorylated (Tyr580) (CAKbeta, cdk-activating Protein Kinase beta)
Purified by immunoaffinity chromatography.
Purified by immunoaffinity chromatography.
Dilution: Western Blot: 1:1000
SDS-Page
(Peptide Competition Extracts prepared from CEFs (1) or CEFs expressing Pyk2 and plated on fibronectin (2-7) were resolved by SDS-PAGE on a 10% polyacrylamide gel and transferred to PVDF. Membranes were blocked with a 5% BSA-TBST buffer overnight at 4oC, then were incubated with Pyk2 [pY580] antibody for two hours at room temperature in a 1% BSA-TBST buffer, following prior incubation with: no peptide (1, 2), the non-phosphopeptide corresponding to the immunogen (3), the phosphopeptide corresponding to Pyk2 [pY579] (4), a phosphopeptide derived from the corresponding region of FAK (5), a generic phosphotyrosine-containing peptide (6) or, the phosphopeptide immunogen (7). After washing, membranes were incubated with goat F(ab’)2 anti-rabbit IgG HRP conjugate and bands were detected using the Pierce SuperSignal‘ method. The data show that only the peptide corresponding to Pyk2 [pY580] completely blocks the antibody signal, thereby demonstrating the specificity of the antibody.)
SDS-Page
(Peptide Competition Extracts prepared from CEFs (1) or CEFs expressing Pyk2 and plated on fibronectin (2-7) were resolved by SDS-PAGE on a 10% polyacrylamide gel and transferred to PVDF. Membranes were blocked with a 5% BSA-TBST buffer overnight at 4oC, then were incubated with Pyk2 [pY580] antibody for two hours at room temperature in a 1% BSA-TBST buffer, following prior incubation with: no peptide (1, 2), the non-phosphopeptide corresponding to the immunogen (3), the phosphopeptide corresponding to Pyk2 [pY579] (4), a phosphopeptide derived from the corresponding region of FAK (5), a generic phosphotyrosine-containing peptide (6) or, the phosphopeptide immunogen (7). After washing, membranes were incubated with goat F(ab’)2 anti-rabbit IgG HRP conjugate and bands were detected using the Pierce SuperSignal‘ method. The data show that only the peptide corresponding to Pyk2 [pY580] completely blocks the antibody signal, thereby demonstrating the specificity of the antibody.)
NCBI and Uniprot Product Information
NCBI Description
This gene encodes a cytoplasmic protein tyrosine kinase which is involved in calcium-induced regulation of ion channels and activation of the map kinase signaling pathway. The encoded protein may represent an important signaling intermediate between neuropeptide-activated receptors or neurotransmitters that increase calcium flux and the downstream signals that regulate neuronal activity. The encoded protein undergoes rapid tyrosine phosphorylation and activation in response to increases in the intracellular calcium concentration, nicotinic acetylcholine receptor activation, membrane depolarization, or protein kinase C activation. This protein has been shown to bind CRK-associated substrate, nephrocystin, GTPase regulator associated with FAK, and the SH2 domain of GRB2. The encoded protein is a member of the FAK subfamily of protein tyrosine kinases but lacks significant sequence similarity to kinases from other subfamilies. Four transcript variants encoding two different isoforms have been found for this gene. [provided by RefSeq, Jul 2008]
Uniprot Description
Pyk2: a nonreceptor tyrosine kinase of the Fak family. Predominantly expressed in the cells derived from hematopoietic lineages and in the central nervous system. Pyk2 is one of the signaling mediators for G-protein-coupled receptors. Involved in calcium induced regulation of ion channel and activation of the map kinase signaling pathway. Interacts with the SH2 domain of Grb2. May phosphorylate the voltage-gated potassium channel protein Kv1.2. Its activation is highly correlated with the stimulation of c-Jun N-terminal kinase activity. It plays an important role in cell motility such as spreading and migration. Two alternatively spliced isoforms have been described.
Protein type: Nuclear receptor co-regulator; Protein kinase, tyrosine (non-receptor); EC 2.7.10.2; Protein kinase, TK; Kinase, protein; TK group; Fak family
Chromosomal Location of Human Ortholog: 8p21.1
Cellular Component: focal adhesion; postsynaptic density; dendrite; cell cortex; cytosol; lipid raft; N-methyl-D-aspartate selective glutamate receptor complex; nucleoplasm; extrinsic to internal side of plasma membrane; growth cone; cytoskeleton; cell soma; perinuclear region of cytoplasm; axon; lamellipodium; cytoplasm; nucleus
Molecular Function: signal transducer activity; protein binding; calmodulin-dependent protein kinase activity; 3-phosphoinositide-dependent protein kinase binding; protein-tyrosine kinase activity; N-methyl-D-aspartate selective glutamate receptor activity; protein complex binding; non-membrane spanning protein tyrosine kinase activity; ATP binding; receptor binding
Biological Process: focal adhesion formation; positive regulation of JNK activity; regulation of nitric oxide biosynthetic process; regulation of cGMP biosynthetic process; protein amino acid phosphorylation; glial cell proliferation; negative regulation of bone mineralization; regulation of cell shape; regulation of inositol trisphosphate biosynthetic process; elevation of cytosolic calcium ion concentration; cell surface receptor linked signal transduction; tumor necrosis factor-mediated signaling pathway; response to glucose stimulus; protein complex assembly; negative regulation of neuron apoptosis; neurite development; bone resorption; response to drug; negative regulation of myeloid cell differentiation; positive regulation of nitric-oxide synthase activity; response to osmotic stress; positive regulation of cell growth; marginal zone B cell differentiation; positive regulation of peptidyl-tyrosine phosphorylation; positive regulation of angiogenesis; response to ethanol; response to mechanical stimulus; ionotropic glutamate receptor signaling pathway; response to calcium ion; negative regulation of apoptosis; activation of JAK protein; peptidyl-tyrosine phosphorylation; response to cAMP; blood vessel endothelial cell migration; positive regulation of translation; apoptosis; protein amino acid autophosphorylation; response to lithium ion; negative regulation of potassium ion transport; response to hormone stimulus; regulation of calcium-mediated signaling; positive regulation of JNK cascade; signal transduction; positive regulation of synaptic transmission, glutamatergic; negative regulation of cell proliferation; positive regulation of cell proliferation; response to stress; angiogenesis; positive regulation of cell-matrix adhesion; epidermal growth factor receptor signaling pathway; regulation of cell adhesion; integrin-mediated signaling pathway; MAPKKK cascade; signal complex assembly; positive regulation of phosphoinositide 3-kinase activity; response to cocaine; oocyte maturation; regulation of release of sequestered calcium ion into cytosol; positive regulation of actin filament polymerization; response to hydrogen peroxide; positive regulation of protein kinase activity; stress fiber formation; response to hypoxia; innate immune response; cellular defense response; sprouting angiogenesis; vascular endothelial growth factor receptor signaling pathway; positive regulation of cell migration
Research Articles on PTK2B
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Product Notes
The PTK2B ptk2b (Catalog #AAA629725) is an Antibody produced from Rabbit and is intended for research purposes only. The product is available for immediate purchase. The Pyk2, phosphorylated (Tyr580) (CAKbeta, cdk-activating Protein Kinase beta) reacts with Human and may cross-react with other species as described in the data sheet. AAA Biotech's Pyk2 can be used in a range of immunoassay formats including, but not limited to, Western Blot (WB). Suitable for use in Western Blot. Dilution: Western Blot: 1:1000. Researchers should empirically determine the suitability of the PTK2B ptk2b for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "Pyk2, Polyclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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