Rabbit ITGB1 / Integrin Beta 1 / CD29 Polyclonal Antibody | anti-ITGB1 antibody

Rabbit Polyclonal to Human ITGB1 / Integrin Beta 1 / CD29

Cat. Num. AAA240185

• Gene Names: ITGB1; CD29; FNRB; MDF2; VLAB; GPIIA; MSK12; VLA-BETA
• Reactivity: Human, Monkey, Bovine, Dog, Horse, Pig, Sheep
• Applications: Immunohistochemistry
• Purity: Immunoaffinity Purified
• Synonyms: ITGB1 / Integrin Beta 1 / CD29; Polyclonal Antibody; Rabbit Polyclonal to Human ITGB1 / Integrin Beta 1 / CD29; Anti-ITGB1 / Integrin Beta 1 / CD29 Antibody (Extracellular Domain) IHC-plus; ITGB1; CD29; CD29 antigen; Integrin beta-1; MDF2; MSK12; Integrin VLA-4 beta subunit; VLA-BETA; VLAB; Beta 1 integrin; FNRB; GPIIA; Integrin Beta 1; VLA-4 subunit beta; Human ITGB1; anti-ITGB1 antibody

• Host: Rabbit
• Reactivity: Human, Monkey, Bovine, Dog, Horse, Pig, Sheep
• Clonality: Polyclonal
• Specificity: Human Integrin Beta 1. BLAST analysis of the peptide immunogen showed no homology with other human proteins.
• Purity/Purification: Immunoaffinity Purified
• Form/Format: PBS, 0.1% sodium azide.
• Concentration: 0.3 mg/ml (varies by lot)
• Sequence Length: 798

• Applicable Applications for anti-ITGB1 antibody: Immunohistochemistry-Paraffin (IHC-P)
• Application Notes: IHC-P (20 ug/ml); ; The unconjugated form was validated for use in immunohistochemistry on a panel of 21 formalin-fixed. paraffin-embedded (FFPE) human tissues after heat induced antigen retrieval in pH 6.0 citrate buffer. After incubation with the primary antibody, slides were incubated with biotinylated secondary antibody, followed by alkaline phosphatase-streptavidin and chromogen. The stained slides were evaluated by a pathologist to confirm staining specificity. The optimal working concentration for the unconjugated form was determined to be 20 ug/ml; ; Optimal dilution to be determined by the researcher.
• Family / Subfamily: Integrin / not assigned-Integrin
• Immunogen Species: ITGB1 / Integrin Beta 1 / CD29 antibody was raised against Human
• Antigen Type: Synthetic Peptide
• Immunogen: ITGB1 / Integrin Beta 1 / CD29 antibody was raised against synthetic 14 amino acid peptide from extracellular domain of human Integrin Beta 1. Percent identity with other species by BLAST analysis: Human, Gorilla, Orangutan, Gibbon, Monkey, Marmoset, Sheep, Dog, Bovine, Cat, Elephant, Panda, Horse, Pig (100%); Platypus (93%); Bat, Opossum (86%).
• Epitope: Extracellular Domain
• Disclaimer: Due to the highly specific nature of antibodies and antigens, we cannot predict or be held responsible with respect to how this antibody will behave in your systems. Researchers using this antibody should conduct optimization studies to achieve the most optimal result possible for their intended application.
• Recommended Immunohistochemistry Protocol: The following protocol is a recommendation only, and MyBioSource, Inc. makes no guarantee of the results:; ; Tissue Preparation: ; Formalin fixation and embedding in paraffin wax. ; ; Tissue Sectioning: ; Make 4-um sections and place on pre-cleaned and charged microscope slides. Heat in a tissue-dryingoven for 45 minutes at 60°C. ; ; Deparaffinization: ; Wash dry slides in 3 changes of xylene - 5 minutes each @ RT; ; Rehydration: ; Wash slides in 3 changes of 100% alcohol - 3 minutes each @ RT; Wash slides in 2 changes of 95% alcohol - 3 minutes each @ RT; Wash slides in 1 change of 80% alcohol - 3 minutes @ RT; Rinse slides in gentle running distilled water - 5 minutes @ RT; ; Antigen retrieval: ; Steam slides in 0.01 M sodium citrate buffer, pH 6.0 at 99-100°C - 20 minutes; Remove from heat and let stand at room temperature in buffer - 20 minutes; Rinse in 1X TBS with Tween (TBST) -1 minute @ RT; ; Immunostaining: ; (Do not allow tissues to dry at any time during the staining procedure) ; Apply a universal protein block - 20 minutes @ RT; Drain protein block from slides, apply diluted primary antibody - 45 minutes @ RT; Rinse slides in 1 X TBST - 1 minute @ RT; Apply a biotinylated secondary antibody appropriate for the primary antibody - 30 minutes @ RT; Rinse slides in 1X TBST -1 minute @ RT; Apply alkaline phosphatase streptavidin - 30 minutes @ RT; Rinse slides in 1X TBST -1 minute @ RT; Apply alkaline phosphatase chromogen substrate - 30 minutes @ RT; Wash slides in distilled water - 1 minute @ RT; ; Dehydrate: ; (This method should only be used if the chromogen substrate is alcohol insoluble (e.g. Vector Red, DAB) ; Wash slides in 2 changes of 80% alcohol - 1 minute each @ RT; Wash slides in 2 changes of 95% alcohol - 1 minute each @ RT; Wash slides in 3 changes of 100% alcohol - 1 minute each @ RT; Wash slides in 3 changes of xylene - 1 minute each @ RT; Apply coverslip
• Preparation and Storage: Aliquot and store undiluted at -20°C or below for up to 1 year.; Can be stored undiluted at 4°C for up to 1 month.; Avoid freeze-thaw cycles.

Immunohistochemistry (IHC)

(Anti-Integrin Beta 1 antibody IHC of human placenta, terminal villi. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval.)

Product Categories/Family for anti-ITGB1 antibody

Family: Integrin

NCBI and Uniprot Product Information

• NCBI GI #: 19743813
• NCBI GeneID: 3688
• NCBI Accession #: NP_002202.2
• NCBI GenBank Nucleotide #: NM_002211.3
• UniProt Accession #: P05556; P78466; P78467; Q13089; Q13090; Q13091; Q13212; A8K6N2; D3DRX9; D3DRY3; D3DRY4; D3DRY5
• NCBI Official Full Name: integrin beta-1 isoform 1A
• NCBI Official Synonym Full Names: integrin, beta 1 (fibronectin receptor, beta polypeptide, antigen CD29 includes MDF2, MSK12)
• NCBI Official Symbol: ITGB1
• NCBI Official Synonym Symbols: CD29; FNRB; MDF2; VLAB; GPIIA; MSK12; VLA-BETA
• NCBI Protein Information: integrin beta-1; glycoprotein IIa; integrin VLA-4 beta subunit; very late activation protein, beta polypeptide
• UniProt Protein Name: Integrin beta-1
• Protein Family: Integrin
• UniProt Gene Name: ITGB1
• UniProt Synonym Gene Names: FNRB; MDF2; MSK12; GPIIA
• UniProt Entry Name: ITB1_HUMAN

NCBI Description

Integrins are heterodimeric proteins made up of alpha and beta subunits. At least 18 alpha and 8 beta subunits have been described in mammals. Integrin family members are membrane receptors involved in cell adhesion and recognition in a variety of processes including embryogenesis, hemostasis, tissue repair, immune response and metastatic diffusion of tumor cells. This gene encodes a beta subunit. Multiple alternatively spliced transcript variants which encode different protein isoforms have been found for this gene. [provided by RefSeq, Jul 2008]

Uniprot Description

ITGB1: an integral membrane protein that heterodimerizes with an alpha-3 chain, forming a receptor for many extracellular-matrix proteins including fibronectin, laminin, collagen, epiligrin and thrombospondin. . Beta 1 integrins recognize the amino-acid motif RGD in a wide array of ligands. Five alternatively spliced variants with alternate carboxy termini have been described. Two alternatively spliced isoforms have been described. Isoform beta-1a is widely expressed; other isoforms are generally expressed with a more restricted distribution. Isoform beta-1b is expressed in skin, liver, skeletal muscle, cardiac muscle, placenta, umbilical vein endothelial cells, neuroblastoma cells, lymphoma cells, hepatoma cells and astrocytoma cells. Isoforms beta-1c and beta-1c-2 are expressed in muscle, kidney, liver, placenta, cervical epithelium, umbilical vein endothelial cells, fibroblast cells, embryonal kidney cells, platelets and several blood cell lines. Isoform beta-c-2, rather than isoform beta-1c, is selectively expressed in primary t-cells. Isoform beta-1c is expressed in nonproliferating and differentiated prostate gland epithelial cells. Isoform beta-1d is expressed specifically in striated muscle (skeletal and cardiac muscle).

Protein type: Motility/polarity/chemotaxis; Membrane protein, integral; Receptor, misc.; Cell surface; Cell adhesion

Chromosomal Location of Human Ortholog: 10p11.2

Cellular Component: focal adhesion; cell surface; dendritic spine; acrosome; lipid raft; ruffle; recycling endosome; membrane; hemidesmosome; lamellipodium; cytoplasm; melanosome; plasma membrane; basement membrane; integrin complex; neuromuscular junction; sarcolemma; receptor complex; cleavage furrow; filopodium; external side of plasma membrane

Molecular Function: protein domain specific binding; protease binding; metal ion binding; laminin binding; alpha-actinin binding; actin binding; protein kinase binding; peptide binding; integrin binding; viral receptor activity; protein binding; protein heterodimerization activity; fibronectin binding; cell adhesion molecule binding; glycoprotein binding

Biological Process: extracellular matrix organization and biogenesis; maternal process involved in pregnancy; axon extension; positive regulation of apoptosis; regulation of cell cycle; mesodermal cell differentiation; positive regulation of endocytosis; cardiac muscle cell differentiation; leukocyte adhesion; transforming growth factor beta receptor signaling pathway; germ cell migration; tissue homeostasis; response to drug; dendrite morphogenesis; protein transport within lipid bilayer; positive regulation of peptidyl-tyrosine phosphorylation; negative regulation of neuron differentiation; cell migration during sprouting angiogenesis; B cell differentiation; cell-cell adhesion mediated by integrin; response to activity; leukocyte migration; regulation of G-protein coupled receptor protein signaling pathway; G1/S transition of mitotic cell cycle; axon guidance; entry of virus into host cell; cell-matrix adhesion; cell fate specification; negative regulation of cell proliferation; positive regulation of MAPKKK cascade; positive regulation of cell proliferation; visual learning; negative regulation of cell projection organization and biogenesis; integrin-mediated signaling pathway; cell migration; regulation of immune response; in utero embryonic development; sarcomere organization; cell-substrate adhesion; formation of radial glial scaffolds; cellular calcium ion homeostasis; heterotypic cell-cell adhesion; negative regulation of Rho protein signal transduction; calcium-independent cell-matrix adhesion; stress fiber formation; cellular defense response; homophilic cell adhesion; blood coagulation; leukocyte tethering or rolling; positive regulation of cell migration