Rabbit anti-Human INHA / Inhibin Alpha Polyclonal Antibody | anti-INHA antibody

Rabbit Polyclonal (IgG) to Human INHA / Inhibin Alpha

Cat. Num. AAA246943

• Reactivity: Human
• Applications: Immunohistochemistry
• Purity: Immunoaffinity Purified
• Synonyms: INHA / Inhibin Alpha; Polyclonal Antibody; Rabbit Polyclonal (IgG) to Human INHA / Inhibin Alpha; Anti-INHA / Inhibin Alpha Antibody IHC-plus; INHA; A-inhibin subunit; Inhibin alpha; Inhibin; alpha; Inhibin alpha chain; Human INHA; Inhibin Alpha; anti-INHA antibody

• Host: Rabbit
• Reactivity: Human
• Clonality: Polyclonal
• Isotype: IgG
• Purity/Purification: Immunoaffinity Purified
• Form/Format: 10 mM PBS, pH 7.4, BSA, sodium azide.
• Sequence Length: 366

• Applicable Applications for anti-INHA antibody: Immunohistochemistry (IHC - Paraffin)
• Application Notes: IHC-P (1:50)
• Family/Subfamily: TGF beta/ not assigned-TGF beta
• Target Species: Human
• Immunogen Description: Synthetic peptide corresponding to human inhibin alpha.
• Immunogen Type: Synthetic peptide
• Immunogen: INHA / Inhibin Alpha antibody was raised against synthetic peptide corresponding to human inhibin alpha.
• Disclaimer: Due to the highly specific nature of antibodies and antigens, we cannot predict or be held responsible with respect to how this antibody will behave in your systems. Researchers using this antibody should conduct optimization studies to achieve the most optimal result possible for their intended application.
• Recommended Immunohistochemistry Protocol: The following protocol is a recommendation only, and MyBioSource, Inc. makes no guarantee of the results:; ; Tissue Preparation: ; Formalin fixation and embedding in paraffin wax. ; ; Tissue Sectioning: ; Make 4-um sections and place on pre-cleaned and charged microscope slides. Heat in a tissue-dryingoven for 45 minutes at 60°C. ; ; Deparaffinization: ; Wash dry slides in 3 changes of xylene - 5 minutes each @ RT; ; Rehydration: ; Wash slides in 3 changes of 100% alcohol - 3 minutes each @ RT; Wash slides in 2 changes of 95% alcohol - 3 minutes each @ RT; Wash slides in 1 change of 80% alcohol - 3 minutes @ RT; Rinse slides in gentle running distilled water - 5 minutes @ RT; ; Antigen retrieval: ; Steam slides in 0.01 M sodium citrate buffer, pH 6.0 at 99-100°C - 20 minutes; Remove from heat and let stand at room temperature in buffer - 20 minutes; Rinse in 1X TBS with Tween (TBST) -1 minute @ RT; ; Immunostaining: ; (Do not allow tissues to dry at any time during the staining procedure) ; Apply a universal protein block - 20 minutes @ RT; Drain protein block from slides, apply diluted primary antibody - 45 minutes @ RT; Rinse slides in 1 X TBST - 1 minute @ RT; Apply a biotinylated secondary antibody appropriate for the primary antibody - 30 minutes @ RT; Rinse slides in 1X TBST -1 minute @ RT; Apply alkaline phosphatase streptavidin - 30 minutes @ RT; Rinse slides in 1X TBST -1 minute @ RT; Apply alkaline phosphatase chromogen substrate - 30 minutes @ RT; Wash slides in distilled water - 1 minute @ RT; ; Dehydrate: ; (This method should only be used if the chromogen substrate is alcohol insoluble (e.g. Vector Red, DAB) ; Wash slides in 2 changes of 80% alcohol - 1 minute each @ RT; Wash slides in 2 changes of 95% alcohol - 1 minute each @ RT; Wash slides in 3 changes of 100% alcohol - 1 minute each @ RT; Wash slides in 3 changes of xylene - 1 minute each @ RT; Apply coverslip
• Preparation and Storage: Store at 2 degree C to 8 degree C degrees. Do not freeze.

Immunohistochemistry (IHC)

(Anti-INHA / Inhibin Alpha antibody IHC of human kidney. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody dilution 1:50.)

Product Categories/Family for anti-INHA antibody

Family: TGF beta

NCBI and Uniprot Product Information

• NCBI GI #: 4504697
• NCBI GeneID: 3623
• NCBI Accession #: NP_002182.1
• NCBI GenBank Nucleotide #: NM_002191.3
• UniProt Accession #: P05111; A8K8H5
• Molecular Weight: 39,670 Da
• NCBI Official Full Name: inhibin alpha chain preproprotein
• NCBI Official Synonym Full Names: inhibin, alpha
• NCBI Official Symbol: INHA
• NCBI Protein Information: inhibin alpha chain; A-inhibin subunit
• UniProt Protein Name: Inhibin alpha chain
• Protein Family: Inhibin
• UniProt Gene Name: INHA
• UniProt Entry Name: INHA_HUMAN

NCBI Description

This gene encodes the alpha subunit of inhibins A and B protein complexes. These complexes negatively regulate follicle stimulating hormone secretion from the pituitary gland. Inhibins have also been implicated in regulating numerous cellular processes including cell proliferation, apoptosis, immune response and hormone secretion.[provided by RefSeq, Dec 2010]

Uniprot Description

INHA: Inhibins and activins inhibit and activate, respectively, the secretion of follitropin by the pituitary gland. Inhibins/activins are involved in regulating a number of diverse functions such as hypothalamic and pituitary hormone secretion, gonadal hormone secretion, germ cell development and maturation, erythroid differentiation, insulin secretion, nerve cell survival, embryonic axial development or bone growth, depending on their subunit composition. Inhibins appear to oppose the functions of activins. Belongs to the TGF-beta family.

Protein type: Secreted, signal peptide; Secreted

Chromosomal Location of Human Ortholog: 2q35

Cellular Component: photoreceptor outer segment; photoreceptor inner segment; cell soma; extracellular region

Molecular Function: protein binding; growth factor activity; protein heterodimerization activity; hormone activity; cytokine activity; transforming growth factor beta receptor binding; receptor binding

Biological Process: negative regulation of B cell differentiation; hemoglobin biosynthetic process; nervous system development; regulation of cell cycle; male gonad development; signal transduction; negative regulation of interferon-gamma biosynthetic process; regulation of cell proliferation; negative regulation of follicle-stimulating hormone secretion; negative regulation of cell cycle; regulation of apoptosis; response to external stimulus; cell surface receptor linked signal transduction; negative regulation of macrophage differentiation; cell-cell signaling; ovarian follicle development; regulation of MAPKKK cascade; erythrocyte differentiation; negative regulation of phosphorylation; positive regulation of follicle-stimulating hormone secretion; cell differentiation; cell cycle arrest; cell development; skeletal development