Mouse anti-Human ICAM1 / CD54 Monoclonal Antibody | anti-ICAM1 antibody

Mouse Monoclonal [clone 15.2] (IgG1) to Human ICAM1 / CD54

Cat. Num. AAA245394

• Gene Names: ICAM1; BB2; CD54; P3.58
• Reactivity: Human
• Applications: Immunohistochemistry, Immunohistochemistry, Immunohistochemistry, Immunoprecipitation, Flow Cytometry, Functional Assay
• Purity: Protein G Purified
• Synonyms: ICAM1 / CD54; Monoclonal Antibody; Mouse Monoclonal [clone 15.2] (IgG1) to Human ICAM1 / CD54; Anti-ICAM1 / CD54 Antibody (clone 15.2) IHC-plus; ICAM1; BB2; CD54 antigen; CD54; ICAM-1; p3.58; Human ICAM1; anti-ICAM1 antibody

• Host: Mouse
• Reactivity: Human
• Clonality: Monoclonal
• Isotype: IgG1
• Clone Number: 15.2
• Specificity: Recognizes the human CD54 cell surface antigen, a 90kD glycoprotein also known as ICAM-1. CD54 is expressed by many cells following activation by inflammatory mediators. Is reported to block CD54 function.
• Purity/Purification: Protein G Purified
• Form/Format: PBS, 0.09% sodium azide.
• Concentration: 1 mg/ml (varies by lot)

• Applicable Applications for anti-ICAM1 antibody: Immunohistochemistry (IHC), Immunohistochemistry-Paraffin (IHC-P), Immunohistochemistry-Frozen (IHC-F), Immunoprecipitation (IP), Flow Cytometry (FC/FACS)
• Application Notes: IHC; IHC - Paraffin (10 ug/ml); IHC - Frozen (1:50-1:100); Immunoprecipitation; Flow Cytometry (1:50 - 1:100); (optimal dilution to be determined by the researcher); ; Immunohistochemistry: MBS245394 was validated for use in immunohistochemistry on a panel of 21 formalin-fixed, paraffin-embedded (FFPE) human tissues after heat induced antigen retrieval in pH 6.0 citrate buffer. After incubation with the primary antibody, slides were incubated with biotinylated secondary antibody, followed by alkaline phosphatase-streptavidin and chromogen. The stained slides were evaluated by a pathologist to confirm staining specificity. The optimal working concentration for MBS245394 was determined to be 10 ug/ml. Flow Cytometry: Use 10 ul of the suggested working dilution to label 10^6 cells in 100ul.
• Family/Subfamily: ICAM/not assigned-ICAM
• Immunogen Species: ICAM-1/CD54 antibody was raised against Human
• Immunogen: ICAM-1/CD54 antibody was raised against human monocytes
• Disclaimer: Due to the highly specific nature of antibodies and antigens, we cannot predict or be held responsible with respect to how this antibody will behave in your systems. Researchers using this antibody should conduct optimization studies to achieve the most optimal result possible for their intended application.
• Recommended Immunohistochemistry Protocol: The following protocol is a recommendation only, and MyBioSource, Inc. makes no guarantee of the results:; ; Tissue Preparation: ; Formalin fixation and embedding in paraffin wax. ; ; Tissue Sectioning: ; Make 4-um sections and place on pre-cleaned and charged microscope slides. Heat in a tissue-dryingoven for 45 minutes at 60°C. ; ; Deparaffinization: ; Wash dry slides in 3 changes of xylene - 5 minutes each @ RT; ; Rehydration: ; Wash slides in 3 changes of 100% alcohol - 3 minutes each @ RT; Wash slides in 2 changes of 95% alcohol - 3 minutes each @ RT; Wash slides in 1 change of 80% alcohol - 3 minutes @ RT; Rinse slides in gentle running distilled water - 5 minutes @ RT; ; Antigen retrieval: ; Steam slides in 0.01 M sodium citrate buffer, pH 6.0 at 99-100°C - 20 minutes; Remove from heat and let stand at room temperature in buffer - 20 minutes; Rinse in 1X TBS with Tween (TBST) -1 minute @ RT; ; Immunostaining: ; (Do not allow tissues to dry at any time during the staining procedure) ; Apply a universal protein block - 20 minutes @ RT; Drain protein block from slides, apply diluted primary antibody - 45 minutes @ RT; Rinse slides in 1 X TBST - 1 minute @ RT; Apply a biotinylated secondary antibody appropriate for the primary antibody - 30 minutes @ RT; Rinse slides in 1X TBST -1 minute @ RT; Apply alkaline phosphatase streptavidin - 30 minutes @ RT; Rinse slides in 1X TBST -1 minute @ RT; Apply alkaline phosphatase chromogen substrate - 30 minutes @ RT; Wash slides in distilled water - 1 minute @ RT; ; Dehydrate: ; (This method should only be used if the chromogen substrate is alcohol insoluble (e.g. Vector Red, DAB) ; Wash slides in 2 changes of 80% alcohol - 1 minute each @ RT; Wash slides in 2 changes of 95% alcohol - 1 minute each @ RT; Wash slides in 3 changes of 100% alcohol - 1 minute each @ RT; Wash slides in 3 changes of xylene - 1 minute each @ RT; Apply coverslip
• Preparation and Storage: Store at 4°C or at -20°C. Store undiluted. Avoid freeze-thaw cycles.; Microcentrifugation recommended if solution contains precipitate.

Immunohistochemistry (IHC)

(Anti-ICAM-1 antibody IHC of human spleen. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody concentration 10 ug/ml.)

Immunohistochemistry (IHC)

(Anti-ICAM-1 antibody IHC of human lung. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody concentration 10 ug/ml.)

Product Categories/Family for anti-ICAM1 antibody

Family: ICAM

NCBI and Uniprot Product Information

• NCBI GI #: 167466198
• NCBI GeneID: 3383
• NCBI Accession #: NP_000192.2
• NCBI GenBank Nucleotide #: NM_000201.2
• UniProt Accession #: P05362; Q5NKV7; Q96B50; B2R6M3
• NCBI Official Full Name: intercellular adhesion molecule 1
• NCBI Official Synonym Full Names: intercellular adhesion molecule 1
• NCBI Official Symbol: ICAM1
• NCBI Official Synonym Symbols: BB2; CD54; P3.58
• NCBI Protein Information: intercellular adhesion molecule 1; ICAM-1; cell surface glycoprotein P3.58; major group rhinovirus receptor; intercellular adhesion molecule 1 (CD54), human rhinovirus receptor
• UniProt Protein Name: Intercellular adhesion molecule 1
• Protein Family: Intercellular adhesion molecule
• UniProt Gene Name: ICAM1
• UniProt Synonym Gene Names: ICAM-1
• UniProt Entry Name: ICAM1_HUMAN

NCBI Description

This gene encodes a cell surface glycoprotein which is typically expressed on endothelial cells and cells of the immune system. It binds to integrins of type CD11a / CD18, or CD11b / CD18 and is also exploited by Rhinovirus as a receptor. [provided by RefSeq, Jul 2008]

Uniprot Description

ICAM1: a type I membrane protein of the immunoglobulin superfamily. Is a ligand for the leukocyte adhesion LFA-1 protein (Integrin alpha-L/beta-2) and a Rhinovirus receptor. Typically expressed on endothelial cells and cells of the immune system. ICAM1 binds to integrins of type CD11a / CD18, or CD11b / CD18. Its expression is activated by p53 in an NF-kappaB-independent manner. Induced by TNFalpha in a process that involves IKKbeta.

Protein type: Immunoglobulin superfamily; Membrane protein, integral; Cell adhesion

Chromosomal Location of Human Ortholog: 19p13.3-p13.2

Cellular Component: extracellular space; cell surface; focal adhesion; membrane; integral to plasma membrane; plasma membrane; immunological synapse; external side of plasma membrane

Molecular Function: viral receptor activity; integrin binding; protein binding; transmembrane receptor activity; receptor activity

Biological Process: extracellular matrix organization and biogenesis; entry of virus into host cell; positive regulation of nitric oxide biosynthetic process; T cell antigen processing and presentation; response to organic cyclic substance; activation of NF-kappaB transcription factor; positive regulation of cellular extravasation; regulation of cell shape; leukocyte adhesion; cellular response to nutrient levels; sensory perception of sound; ovarian follicle development; T cell activation via T cell receptor contact with antigen bound to MHC molecule on antigen presenting cell; membrane to membrane docking; response to sulfur dioxide; cell adhesion; regulation of leukocyte mediated cytotoxicity; acute inflammatory response to antigenic stimulus; response to drug; regulation of cell adhesion; virion attachment, binding of host cell surface receptor; regulation of immune response; negative regulation of calcium ion transport; cytokine and chemokine mediated signaling pathway; response to amphetamine; cell aging; response to amino acid stimulus; heterophilic cell adhesion; positive regulation of peptidyl-tyrosine phosphorylation; response to ethanol; response to copper ion; positive regulation of actin filament polymerization; positive regulation of vasoconstriction; cell adhesion mediated by integrin; adhesion to host; response to ionizing radiation; leukocyte migration

Disease: Malaria, Susceptibility To