Goat SPI1 / PU.1 Polyclonal Antibody | anti-SPI1 antibody

Goat Polyclonal to Human SPI1 / PU.1

Cat. Num. AAA247864

• Gene Names: SPI1; OF; PU.1; SFPI1; SPI-1; SPI-A
• Reactivity: Human, Bovine, Dog, Horse, Pig, Rabbit; Predicted Reactivity: Monkey
• Applications: Immunohistochemistry
• Purity: Immunoaffinity Purified
• Synonyms: SPI1 / PU.1; Polyclonal Antibody; Goat Polyclonal to Human SPI1 / PU.1; Anti-SPI1 / PU.1 Antibody IHC-plus; SPI1; 31 kDa-transforming protein; 31 kDa transforming protein; PU.1; SFPI1; SPI-1; SPI-1 proto-oncogene; SPI-A; Transcription factor PU.1; Human SPI1; anti-SPI1 antibody

• Host: Goat
• Reactivity: Human, Bovine, Dog, Horse, Pig, Rabbit; Predicted Reactivity: Monkey
• Clonality: Polyclonal
• Specificity: Human SPI1 / PU.1. This antibody is expected to recognise both reported isoforms (NP_001074016.1; NP_003111.2).
• Purity/Purification: Immunoaffinity Purified
• Form/Format: TBS, pH7.3, 0.02% Sodium Azide, 0.5% BSA
• Sequence Positions: 27-37

• Applicable Applications for anti-SPI1 antibody: Immunohistochemistry (IHC - Paraffin)
• Application Notes: IHC-P (3.75 ug/ml); Optimal dilutons to be determined by the researcher.
• Immunogen Species: Human
• Antigen Type: Synthetic peptide
• Immunogen: SPI1 / PU.1 antibody was raised against synthetic peptide C-DLYQRQTHEYY from an internal region of human SPI1 / PU.1 (NP_001074016.1; NP_003111.2). Percent identity by BLAST analysis: Human, Gorilla, Gibbon, Monkey, Panda, Bovine, Dog, Horse, Rabbit, Pig (100%); Marmoset, Opossum (91%); Rat, Platypus (82%).
• Usage Summary: Immunohistochemistry: MBS247864 was validated for use in immunohistochemistry on a panel of 21 formalin-fixed, paraffin-embedded (FFPE) human tissues after heat induced antigen retrieval in pH 6.0 citrate buffer. After incubation with the primary antibody, slides were incubated with biotinylated secondary antibody, followed by alkaline phosphatase-streptavidin and chromogen. The stained slides were evaluated by a pathologist to confirm staining specificity.The optimal working concentration for MBS247864 was determined to be 3.75ug/ml.
• Disclaimer: Due to the highly specific nature of antibodies and antigens, we cannot predict or be held responsible with respect to how this antibody will behave in your systems. Researchers using this antibody should conduct optimization studies to achieve the most optimal result possible for their intended application.
• Recommended Immunohistochemistry Protocol: The following protocol is a recommendation only, and MyBioSource, Inc. makes no guarantee of the results:; ; Tissue Preparation: ; Formalin fixation and embedding in paraffin wax. ; ; Tissue Sectioning: ; Make 4-um sections and place on pre-cleaned and charged microscope slides. Heat in a tissue-dryingoven for 45 minutes at 60°C. ; ; Deparaffinization: ; Wash dry slides in 3 changes of xylene - 5 minutes each @ RT; ; Rehydration: ; Wash slides in 3 changes of 100% alcohol - 3 minutes each @ RT; Wash slides in 2 changes of 95% alcohol - 3 minutes each @ RT; Wash slides in 1 change of 80% alcohol - 3 minutes @ RT; Rinse slides in gentle running distilled water - 5 minutes @ RT; ; Antigen retrieval: ; Steam slides in 0.01 M sodium citrate buffer, pH 6.0 at 99-100°C - 20 minutes; Remove from heat and let stand at room temperature in buffer - 20 minutes; Rinse in 1X TBS with Tween (TBST) -1 minute @ RT; ; Immunostaining: ; (Do not allow tissues to dry at any time during the staining procedure) ; Apply a universal protein block - 20 minutes @ RT; Drain protein block from slides, apply diluted primary antibody - 45 minutes @ RT; Rinse slides in 1 X TBST - 1 minute @ RT; Apply a biotinylated secondary antibody appropriate for the primary antibody - 30 minutes @ RT; Rinse slides in 1X TBST -1 minute @ RT; Apply alkaline phosphatase streptavidin - 30 minutes @ RT; Rinse slides in 1X TBST -1 minute @ RT; Apply alkaline phosphatase chromogen substrate - 30 minutes @ RT; Wash slides in distilled water - 1 minute @ RT; ; Dehydrate: ; (This method should only be used if the chromogen substrate is alcohol insoluble (e.g. Vector Red, DAB) ; Wash slides in 2 changes of 80% alcohol - 1 minute each @ RT; Wash slides in 2 changes of 95% alcohol - 1 minute each @ RT; Wash slides in 3 changes of 100% alcohol - 1 minute each @ RT; Wash slides in 3 changes of xylene - 1 minute each @ RT; Apply coverslip
• Preparation and Storage: Store at -20 degree C. Minimize freezing and thawing.

Immunohistochemistry (IHC)

(Anti-SPI1 / PU.1 antibody IHC of human spleen. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody concentration 3.75 ug/ml.)

Immunohistochemistry (IHC)

(Anti-SPI1 / PU.1 antibody IHC of human colon, lymphoid aggregate. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody concentration 3.75 ug/ml.)

NCBI and Uniprot Product Information

• NCBI GI #: 124028517
• NCBI GeneID: 6688
• NCBI Accession #: NP_003111.2
• NCBI GenBank Nucleotide #: NM_003120.2
• UniProt Accession #: P17947
• Molecular Weight: 31,211 Da
• NCBI Official Full Name: transcription factor PU.1 isoform 2
• NCBI Official Synonym Full Names: Spi-1 proto-oncogene
• NCBI Official Symbol: SPI1
• NCBI Official Synonym Symbols: OF; PU.1; SFPI1; SPI-1; SPI-A
• NCBI Protein Information: transcription factor PU.1; 31 kDa transforming protein; 31 kDa-transforming protein; hematopoietic transcription factor PU.1; spleen focus forming virus (SFFV) proviral integration oncogene spi1
• UniProt Protein Name: Transcription factor PU.1
• Protein Family: Transcription factor
• UniProt Gene Name: SPI1
• UniProt Entry Name: SPI1_HUMAN

NCBI Description

This gene encodes an ETS-domain transcription factor that activates gene expression during myeloid and B-lymphoid cell development. The nuclear protein binds to a purine-rich sequence known as the PU-box found near the promoters of target genes, and regulates their expression in coordination with other transcription factors and cofactors. The protein can also regulate alternative splicing of target genes. Multiple transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, Jul 2008]

Uniprot Description

PU.1: Binds to the PU-box, a purine-rich DNA sequence (5'- GAGGAA-3') that can act as a lymphoid-specific enhancer. This protein is a transcriptional activator that may be specifically involved in the differentiation or activation of macrophages or B- cells. Also binds RNA and may modulate pre-mRNA splicing. Binds DNA as a monomer. Interacts with CEBPD and NONO. Interacts with RUNX1 and SPIB. Interacts with GFI1; the interaction represses SPI1 transcriptional activity. Highly expressed in both FV-P and FV-A-induced erythro- leukemia cell lines that have undergone rearrangements of the Spi- 1 gene due to the insertion of SFFV. Belongs to the ETS family. 2 isoforms of the human protein are produced by alternative splicing.

Protein type: Transcription factor; DNA-binding; Oncoprotein

Chromosomal Location of Human Ortholog: 11p11.2

Cellular Component: nuclear chromatin

Molecular Function: RNA polymerase II transcription factor activity, enhancer binding; protein binding; NFAT protein binding; RNA binding; transcription factor activity

Biological Process: transcription from RNA polymerase II promoter; macrophage differentiation; granulocyte differentiation; somatic stem cell maintenance; positive regulation of transcription, DNA-dependent; lymphoid progenitor cell differentiation; myeloid dendritic cell differentiation; negative regulation of transcription from RNA polymerase II promoter; anatomical structure regression; regulation of erythrocyte differentiation; regulation of transcription from RNA polymerase II promoter; lymphocyte differentiation; negative regulation of gene expression, epigenetic; negative regulation of MHC class II biosynthetic process; erythrocyte differentiation; positive regulation of transcription from RNA polymerase II promoter; negative regulation of transcription, DNA-dependent