Mouse anti-Human FOXO3 / FOXO3A Monoclonal Antibody | anti-FOXO3 antibody

Mouse Monoclonal [clone 2H2] (IgG2a,k) to Human FOXO3 / FOXO3A

Cat. Num. AAA245568

• Gene Names: FOXO3; FOXO2; AF6q21; FKHRL1; FOXO3A; FKHRL1P2
• Reactivity: Human
• Applications: Immunohistochemistry, Immunofluorescence, Western Blot, ELISA
• Purity: Purified from ascites by Protein A
• Synonyms: FOXO3 / FOXO3A; Monoclonal Antibody; Mouse Monoclonal [clone 2H2] (IgG2a; k) to Human FOXO3 / FOXO3A; Anti-FOXO3 / FOXO3A Antibody (clone 2H2) IHC-plus; FOXO3; AF6q21; AF6q21 protein; Forkhead box O3; Forkhead box O3A; FOXO3A; FKHRL1P2; Forkhead box protein O3; FOXO2; FKHRL1; Human FOXO3; anti-FOXO3 antibody

• Host: Mouse
• Reactivity: Human
• Clonality: Monoclonal
• Isotype: IgG2a,k
• Clone Number: 2H2
• Specificity: Human FOXO3
• Purity/Purification: Purified from ascites by Protein A
• Form/Format: PBS, pH 7.4
• Concentration: 0.21 mg/ml (varies by lot)
• Sequence Length: 673

• Applicable Applications for anti-FOXO3 antibody: Immunohistochemistry (IHC - Paraffin), Immunofluorescence (IF), Western Blot (WB), ELISA (EIA)
• Application Notes: IHC- Paraffin (5ug/ml); Immunofluorescence (10ug/ml); Western Blot (1:500-1:1000); Usage: Immunohistochemistry: Formalin-fixed paraffin-embedded sections. Sandwich ELISA: Recombinant protein. Western Blot: Recombinant protein.
• Target: FOXO3/FOXO3A
• Family/Subfamily: Transcription factor/ not assigned- Transcription factor
• Immunogen species: FOXO3/FOXX3A antibody was raised against human
• Antigen Type: Recombinant protein
• Immunogen: FOXO3 / FOXO3A antibody was raised against fOXO3A (AAH21224, 361 a.a. ~ 460 a.a) partial recombinant protein with GST tag. MW of the GST tag alone is 26 KDa.
• Disclaimer: Due to the highly specific nature of antibodies and antigens, we cannot predict or be held responsible with respect to how this antibody will behave in your systems. Researchers using this antibody should conduct optimization studies to achieve the most optimal result possible for their intended application.
• Recommended Immunohistochemistry Protocol: The following protocol is a recommendation only, and MyBioSource, Inc. makes no guarantee of the results:; ; Tissue Preparation: ; Formalin fixation and embedding in paraffin wax. ; ; Tissue Sectioning: ; Make 4-um sections and place on pre-cleaned and charged microscope slides. Heat in a tissue-dryingoven for 45 minutes at 60°C. ; ; Deparaffinization: ; Wash dry slides in 3 changes of xylene - 5 minutes each @ RT; ; Rehydration: ; Wash slides in 3 changes of 100% alcohol - 3 minutes each @ RT; Wash slides in 2 changes of 95% alcohol - 3 minutes each @ RT; Wash slides in 1 change of 80% alcohol - 3 minutes @ RT; Rinse slides in gentle running distilled water - 5 minutes @ RT; ; Antigen retrieval: ; Steam slides in 0.01 M sodium citrate buffer, pH 6.0 at 99-100°C - 20 minutes; Remove from heat and let stand at room temperature in buffer - 20 minutes; Rinse in 1X TBS with Tween (TBST) -1 minute @ RT; ; Immunostaining: ; (Do not allow tissues to dry at any time during the staining procedure) ; Apply a universal protein block - 20 minutes @ RT; Drain protein block from slides, apply diluted primary antibody - 45 minutes @ RT; Rinse slides in 1 X TBST - 1 minute @ RT; Apply a biotinylated secondary antibody appropriate for the primary antibody - 30 minutes @ RT; Rinse slides in 1X TBST -1 minute @ RT; Apply alkaline phosphatase streptavidin - 30 minutes @ RT; Rinse slides in 1X TBST -1 minute @ RT; Apply alkaline phosphatase chromogen substrate - 30 minutes @ RT; Wash slides in distilled water - 1 minute @ RT; ; Dehydrate: ; (This method should only be used if the chromogen substrate is alcohol insoluble (e.g. Vector Red, DAB) ; Wash slides in 2 changes of 80% alcohol - 1 minute each @ RT; Wash slides in 2 changes of 95% alcohol - 1 minute each @ RT; Wash slides in 3 changes of 100% alcohol - 1 minute each @ RT; Wash slides in 3 changes of xylene - 1 minute each @ RT; Apply coverslip
• Preparation and Storage: Upon arrival: Aliquot and store at -20 degree C or -80 degree C. Avoid freeze-thaw cycles.

Immunohistochemistry (IHC)

(Anti-FOXO3A antibody IHC of human spleen. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody concentration 5 ug/ml.)

Immunofluorescence (IF)

(Immunofluorescence of monoclonal antibody to FOXO3 on HeLa cell. [antibody concentration 10 ug/ml].)

ELISA (EIA)

(Detection limit for recombinant GST tagged FOXO3A is approximately 1 ng/ml as a capture antibody.)

Product Categories/Family for anti-FOXO3 antibody

Family: Transcription factor

NCBI and Uniprot Product Information

• NCBI GI #: 4503739
• NCBI GeneID: 2309
• NCBI Accession #: NP_001446.1
• NCBI GenBank Nucleotide #: NM_001455.3
• UniProt Accession #: O43524; O15171; Q5T2I7; Q9BZ04; B4DVZ6; E1P5E6
• Molecular Weight: 48,406 Da
• NCBI Official Full Name: forkhead box protein O3
• NCBI Official Synonym Full Names: forkhead box O3
• NCBI Official Symbol: FOXO3
• NCBI Official Synonym Symbols: FOXO2; AF6q21; FKHRL1; FOXO3A; FKHRL1P2
• NCBI Protein Information: forkhead box protein O3; forkhead box O3A; forkhead in rhabdomyosarcoma-like 1; forkhead homolog (rhabdomyosarcoma) like 1; forkhead, Drosophila, homolog of, in rhabdomyosarcoma-like 1
• UniProt Protein Name: Forkhead box protein O3
• Protein Family: Forkhead box protein
• UniProt Gene Name: FOXO3
• UniProt Synonym Gene Names: FKHRL1; FOXO3A
• UniProt Entry Name: FOXO3_HUMAN

NCBI Description

This gene belongs to the forkhead family of transcription factors which are characterized by a distinct forkhead domain. This gene likely functions as a trigger for apoptosis through expression of genes necessary for cell death. Translocation of this gene with the MLL gene is associated with secondary acute leukemia. Alternatively spliced transcript variants encoding the same protein have been observed. [provided by RefSeq, Jul 2008]

Uniprot Description

FOXO3A: a transcription factors of the forkhead family. Contains 1 fork-head domain. May regulate apoptosis through control of genes necessary for cell death. Translocation of this gene with the MLL gene is associated with secondary acute leukemia.

Protein type: DNA-binding; Transcription factor; Autophagy; Oncoprotein

Chromosomal Location of Human Ortholog: 6q21

Cellular Component: nucleoplasm; membrane; cytoplasm; cytosol; nucleus

Molecular Function: protein binding; DNA binding; sequence-specific DNA binding; chromatin DNA binding; transcription factor activity; protein kinase binding

Biological Process: transcription from RNA polymerase II promoter; epidermal growth factor receptor signaling pathway; phosphoinositide-mediated signaling; fibroblast growth factor receptor signaling pathway; nerve growth factor receptor signaling pathway; DNA damage response, signal transduction by p53 class mediator; initiation of primordial ovarian follicle growth; positive regulation of transcription, DNA-dependent; antral ovarian follicle growth; positive regulation of erythrocyte differentiation; negative regulation of transcription from RNA polymerase II promoter; glucose homeostasis; ovulation from ovarian follicle; oocyte maturation; regulation of transcription from RNA polymerase II promoter; regulation of translation; positive regulation of neuron apoptosis; innate immune response; positive regulation of transcription from RNA polymerase II promoter