Goat SLC7A11 / XCT Polyclonal Antibody | anti-SLC7A11 antibody

Goat Polyclonal to Human SLC7A11 / XCT

Cat. Num. AAA247785

• Gene Names: SLC7A11; xCT; CCBR1
• Reactivity: Human, Monkey
• Applications: Immunohistochemistry, Western Blot, ELISA
• Purity: Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
• Synonyms: SLC7A11 / XCT; Polyclonal Antibody; Goat Polyclonal to Human SLC7A11 / XCT; Anti-SLC7A11 / XCT Antibody (Internal) IHC-plus; SLC7A11; CCBR1; Cystine/glutamate transporter; XCT; Human SLC7A11; anti-SLC7A11 antibody

• Host: Goat
• Reactivity: Human, Monkey
• Clonality: Polyclonal
• Specificity: Human SLC7A11.
• Purity/Purification: Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
• Form/Format: TBS, pH 7.3, 0.5% BSA, 0.02% sodium azide
• Concentration: 0.5 mg/ml (varies by lot)
• Sequence Length: 501

• Applicable Applications for anti-SLC7A11 antibody: Immunohistochemistry (IHC - Paraffin), Western Blot (WB), ELISA (EIA)
• Application Notes: ELISA (1:128000), IHC-P (3.75 ug/ml), WB (0.02 - 0.06 ug/ml)
• Target Species: Human
• Epitope: aa215-229
• Family/Subfamily: Transporter / Cationic amino acid transporter
• Immunogen: SLC7A11/ XCT antibody was raised against peptide with sequence C-KGQTQNFKDAFSGRD, from the internal region of the protein sequence according to NP_055146.1
• Disclaimer: Due to the highly specific nature of antibodies and antigens, we cannot predict or be held responsible with respect to how this antibody will behave in your systems. Researchers using this antibody should conduct optimization studies to achieve the most optimal result possible for their intended application.
• Recommended Immunohistochemistry Protocol: The following protocol is a recommendation only, and MyBioSource, Inc. makes no guarantee of the results:; ; Tissue Preparation: ; Formalin fixation and embedding in paraffin wax. ; ; Tissue Sectioning: ; Make 4-um sections and place on pre-cleaned and charged microscope slides. Heat in a tissue-dryingoven for 45 minutes at 60°C. ; ; Deparaffinization: ; Wash dry slides in 3 changes of xylene - 5 minutes each @ RT; ; Rehydration: ; Wash slides in 3 changes of 100% alcohol - 3 minutes each @ RT; Wash slides in 2 changes of 95% alcohol - 3 minutes each @ RT; Wash slides in 1 change of 80% alcohol - 3 minutes @ RT; Rinse slides in gentle running distilled water - 5 minutes @ RT; ; Antigen retrieval: ; Steam slides in 0.01 M sodium citrate buffer, pH 6.0 at 99-100°C - 20 minutes; Remove from heat and let stand at room temperature in buffer - 20 minutes; Rinse in 1X TBS with Tween (TBST) -1 minute @ RT; ; Immunostaining: ; (Do not allow tissues to dry at any time during the staining procedure) ; Apply a universal protein block - 20 minutes @ RT; Drain protein block from slides, apply diluted primary antibody - 45 minutes @ RT; Rinse slides in 1 X TBST - 1 minute @ RT; Apply a biotinylated secondary antibody appropriate for the primary antibody - 30 minutes @ RT; Rinse slides in 1X TBST -1 minute @ RT; Apply alkaline phosphatase streptavidin - 30 minutes @ RT; Rinse slides in 1X TBST -1 minute @ RT; Apply alkaline phosphatase chromogen substrate - 30 minutes @ RT; Wash slides in distilled water - 1 minute @ RT; ; Dehydrate: ; (This method should only be used if the chromogen substrate is alcohol insoluble (e.g. Vector Red, DAB) ; Wash slides in 2 changes of 80% alcohol - 1 minute each @ RT; Wash slides in 2 changes of 95% alcohol - 1 minute each @ RT; Wash slides in 3 changes of 100% alcohol - 1 minute each @ RT; Wash slides in 3 changes of xylene - 1 minute each @ RT; Apply coverslip
• Preparation and Storage: Aliquot and store at -20°C. Avoid freeze-thaw cycles.

Immunohistochemistry (IHC)

(Anti-SLC7A11 antibody IHC of human kidney. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody concentration 3.75 ug/ml.)

Immunohistochemistry (IHC)

(Anti-SLC7A11 antibody IHC of human placenta. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody concentration 3.75 ug/ml.)

Product Categories/Family for anti-SLC7A11 antibody

Family: Transporter; Subfamily: Cationic amino acid transporter

NCBI and Uniprot Product Information

• NCBI GI #: 7657683
• NCBI GeneID: 23657
• NCBI Accession #: NP_055146.1
• NCBI GenBank Nucleotide #: NM_014331.3
• UniProt Accession #: Q9UPY5; A8K2U4
• NCBI Official Full Name: cystine/glutamate transporter
• NCBI Official Synonym Full Names: solute carrier family 7 (anionic amino acid transporter light chain, xc- system), member 11
• NCBI Official Symbol: SLC7A11
• NCBI Official Synonym Symbols: xCT; CCBR1
• NCBI Protein Information: cystine/glutamate transporter; amino acid transport system xc-; solute carrier family 7 member 11; calcium channel blocker resistance protein CCBR1; solute carrier family 7, (cationic amino acid transporter, y+ system) member 11
• UniProt Protein Name: Cystine/glutamate transporter
• Protein Family: Cystine/glutamate transporter
• UniProt Gene Name: SLC7A11
• UniProt Entry Name: XCT_HUMAN

NCBI Description

This gene encodes a member of a heteromeric, sodium-independent, anionic amino acid transport system that is highly specific for cysteine and glutamate. In this system, designated Xc(-), the anionic form of cysteine is transported in exchange for glutamate. This protein has been identified as the predominant mediator of Kaposi sarcoma-associated herpesvirus fusion and entry permissiveness into cells. Also, increased expression of this gene in primary gliomas (compared to normal brain tissue) was associated with increased glutamate secretion via the XCT channels, resulting in neuronal cell death. [provided by RefSeq, Sep 2011]

Uniprot Description

SLC7A11: Sodium-independent, high-affinity exchange of anionic amino acids with high specificity for anionic form of cystine and glutamate. Belongs to the amino acid-polyamine-organocation (APC) superfamily. L-type amino acid transporter (LAT) (TC 2.A.3.8) family.

Protein type: Membrane protein, multi-pass; Transporter; Transporter, SLC family; Membrane protein, integral

Chromosomal Location of Human Ortholog: 4q28.3

Cellular Component: cell surface; cytoskeleton; rough endoplasmic reticulum; plasma membrane; integral to membrane

Molecular Function: protein binding; cystine:glutamate antiporter activity

Biological Process: response to nicotine; amino acid transport; response to toxin; ion transport; response to oxidative stress; brain development; blood coagulation; transmembrane transport; leukocyte migration