Imunnohistochemistry (IHC)
( Human lung tissue was immunce-stained with Rabbit anti IRAK-M antibody (MBS460311) at 1:200 for 10 min @ RT. Staining of formalin-fixed tissue requires boiling tissue sections in 10mM Citrate Buffer, pH 6.0 for 10 min followed by cooling at RT for 20 min.)
Rabbit IRAK-M Polyclonal Antibody | anti-IRAK-M antibody
Rabbit anti IRAK-M Polyclonal Antibody
Reactivity
Human, Mouse, Rat
Applications
ELISA, Flow Cytometry, Functional Assay, Immunohistochemistry, Immunoprecipitation, Western Blot
Purity
Purified by Epitope Affinity Purification
Synonyms
IRAK-M; Polyclonal Antibody; Rabbit anti IRAK-M Polyclonal Antibody; Interleukin-1 receptor-associated kinase 3; IRAK-3; anti-IRAK-M antibody
Host
Rabbit
Reactivity
Human, Mouse, Rat
Clonality
Polyclonal
Isotype
IgG
Specificity
This antibody recognizes IRAK-M.
Purity/Purification
Purified by Epitope Affinity Purification
Form/Format
Supplied in sterile Phosphate buffered saline (pH7.2) containing antibody stabilizer.
Applicable Applications for anti-IRAK-M antibody
ELISA (EIA), Flow Cytometry (FC/FACS), Immunohistochemistry (IHC), Immunoprecipitation (IP), Western Blot (WB)
Application Notes
ELISA: 0.01-0.1 ug/ml
Immunohistochemistry: 2-5 ug/ml
Immunoprecipitation: 2-5 ug/ml
Western Blot: 0.1-1 ug/ml
Immunohistochemistry: 2-5 ug/ml
Immunoprecipitation: 2-5 ug/ml
Western Blot: 0.1-1 ug/ml
Positive Control
Raji Cells
Cellular Location
Cytoplasmic
Antigen Preparation
A synthetic peptide derived from C-terminus of human IRAK-M protein was used as part of immunogen. This sequence is identicalto human, mouse and rat.
Preparation and Storage
The antibodies are stable for 12 months from date of receipt when stored at -20 degree C. The antibodies can be stored at 2 degree C - 8 degree C for one month without detectable loss of activity. Avoid repeated freezing-thawing cycles.
Imunnohistochemistry (IHC)
( Human lung tissue was immunce-stained with Rabbit anti IRAK-M antibody (MBS460311) at 1:200 for 10 min @ RT. Staining of formalin-fixed tissue requires boiling tissue sections in 10mM Citrate Buffer, pH 6.0 for 10 min followed by cooling at RT for 20 min.)
Imunnohistochemistry (IHC)
( Human lung tissue was immunce-stained with Rabbit anti IRAK-M antibody (MBS460311) at 1:200 for 10 min @ RT. Staining of formalin-fixed tissue requires boiling tissue sections in 10mM Citrate Buffer, pH 6.0 for 10 min followed by cooling at RT for 20 min.)
Related Product Information for anti-IRAK-M antibody
IRAK (IL-1 associated serine/threonine kinase) is critical regulator of interleukin-1 (IL-1) induced activation of the NFkappaA pathway. IL-1 stimulation leads to recruitment of interleukin-1 receptor-associated kinase (IRAK) to the IL-1 receptor, where IRAK is phosphorylated, ubiquitinated, and eventually degraded. There are four members (IRAK1, IRAK2, IRAK3-IRAKM and IRAK4). A novel member in the IRAK/Pelle was recently identified and designated IRAK-M. IRAKs associate with IL-1/Toll receptors after IL-1 or LPS stimulation and the dominant negative mutants or IRAKs inhibit IL-1 or LPS induced NF-kB activation. Members in IRAK/Pelle family play a central role in IL-1R/TLR mediated inflammatory responses to cytokine IL-1 and LPS.
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Product Notes
The IRAK-M (Catalog #AAA460311) is an Antibody produced from Rabbit and is intended for research purposes only. The product is available for immediate purchase. The Rabbit anti IRAK-M Polyclonal Antibody reacts with Human, Mouse, Rat and may cross-react with other species as described in the data sheet. AAA Biotech's IRAK-M can be used in a range of immunoassay formats including, but not limited to, ELISA (EIA), Flow Cytometry (FC/FACS), Immunohistochemistry (IHC), Immunoprecipitation (IP), Western Blot (WB). ELISA: 0.01-0.1 ug/ml Immunohistochemistry: 2-5 ug/ml Immunoprecipitation: 2-5 ug/ml Western Blot: 0.1-1 ug/ml. Researchers should empirically determine the suitability of the IRAK-M for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "IRAK-M, Polyclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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