Rabbit anti-Human IRAK-2 Polyclonal Antibody | anti-IRAK2 antibody

IRAK-2 Antibody

Cat. Num. AAA151252

• Gene Names: IRAK2; IRAK-2
• Reactivity: Human
• Applications: ELISA, Western Blot, Immunocytochemistry, Immunofluorescence
• Purity: IRAK-2 Antibody is affinity chromatography purified via peptide column.
• Synonyms: IRAK-2; Polyclonal Antibody; IRAK-2 Antibody; Interleukin-1 receptor-associated kinase-like 2; interleukin-1 receptor-associated kinase 2; anti-IRAK2 antibody

• Host: Rabbit
• Reactivity: Human
• Clonality: Polyclonal
• Isotype: IgG
• Specificity: Anti-IRAK2 has no cross response to IRAK.
• Purity/Purification: IRAK-2 Antibody is affinity chromatography purified via peptide column.
• Form/Format: Liquid
• Sequence Length: 1782

• Applicable Applications for anti-IRAK2 antibody: ELISA (EIA), Western Blot (WB), Immunocytochemistry (ICC), Immunofluorescence (IF)
• Application Notes: IRAK-2 antibody can be used for detection of IRAK2 by Western blot at 2 mug/mL. A 65 kDa band should be detected. This polyclonal antibody can also detect IRAK2 by immunocytochemistry at 2 mug/mL. For immunofluorescence start at 10 mug/mL.
• Conjugate: Unconjugated
• Immunogen: IRAK2 antibody was raised against a peptide corresponding to amino acids near the carboxy terminus of human IRAK2.
• Buffer: IRAK-2 Antibody is supplied in PBS containing 0.02% sodium azide.
• Preparation and Storage: IRAK-2 antibody can be stored at 4 degree C for three months and -20 degree C, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures.

Western Blot (WB)

(Western blot analysis of IRAK2 in HeLa (H) and K562 (K) whole cell lysate with IRAK2 antibody (C2) at 2 μg/mL.)

Immunocytochemistry (IHC)

(Immunocytochemical staining of HeLa cells using IRAK2 antibody at 2 μg/mL.)

Immunofluorescence (IF)

(Immunofluorescence of IRAK-2 in Hela cells with IRAK-2 antibody at 10 μg/mL.)

Immunofluorescence (IF)

(Immunofluorescence of IRAK-M in human spleen tissue with IRAK M antibody at 20 μg/mL.Green: IRAK-2 Antibody (2123)Blue: DAPI staining)

Related Product Information for anti-IRAK2 antibody

IRAK-2 Antibody: The pro-inflammatory cytokine IL-1 induces cellular response through two subunits of its receptor, IL-1 receptor I (IL-1RI) and IL-1 receptor accessory protein (IL-1RAcP). IL-1 receptor-associated kinase (IRAK) mediates activation of NF-κB, which is a pivotal transcription factor mediating inflammatory and immune response. A novel member in the IRAK/Pelle family was recently identified and designated IRAK2. Both IRAK and IRAK2 recruit to the subunits of the IL-1R complex after IL-1 binding and lead to NF-κB activation. IRAKs also associate with Toll like receptor (TLR) and the dominant negative mutants of IRAKs inhibit LPS-induced NF-κB activation. Members in IRAK/Pelle family play a central role in IL-1R and TLR mediated inflammatory response. IRAK2 is expressed in a variety of human tissues.

NCBI and Uniprot Product Information

• NCBI GI #: 2653876
• NCBI GeneID: 3656
• UniProt Accession #: O43187; Q08AG6; Q5K546; B4DQZ6
• Molecular Weight: 65 kDa
• NCBI Official Full Name: Homo sapiens interleukin-1 receptor-associated kinase-2 mRNA, complete cds
• NCBI Official Synonym Full Names: interleukin-1 receptor-associated kinase 2
• NCBI Official Symbol: IRAK2
• NCBI Official Synonym Symbols: IRAK-2
• NCBI Protein Information: interleukin-1 receptor-associated kinase-like 2; interleukin-1 receptor associated kinase-2
• UniProt Protein Name: Interleukin-1 receptor-associated kinase-like 2
• Protein Family: Interleukin-1 receptor-associated kinase
• UniProt Gene Name: IRAK2
• UniProt Synonym Gene Names: IRAK-2
• UniProt Entry Name: IRAK2_HUMAN

NCBI Description

IRAK2 encodes the interleukin-1 receptor-associated kinase 2, one of two putative serine/threonine kinases that become associated with the interleukin-1 receptor (IL1R) upon stimulation. IRAK2 is reported to participate in the IL1-induced upregulation of NF-kappaB. [provided by RefSeq, Jul 2008]

Uniprot Description

IRAK2: Binds to the IL-1 type I receptor following IL-1 engagement, triggering intracellular signaling cascades leading to transcriptional up-regulation and mRNA stabilization. Interacts with MYD88. IL-1 stimulation leads to the formation of a signaling complex which dissociates from the IL-1 receptor following the binding of PELI1. Expressed in spleen, thymus, prostate, lung, liver, skeletal muscle, kidney, pancreas and peripheral blood leukocytes. Belongs to the protein kinase superfamily. TKL Ser/Thr protein kinase family. Pelle subfamily.

Protein type: Protein kinase, Ser/Thr (non-receptor); Kinase, protein; Protein kinase, TKL; TKL group; IRAK family

Chromosomal Location of Human Ortholog: 3p25.3

Cellular Component: interleukin-1 receptor complex; cytoplasm; plasma membrane; endosome membrane; cytosol; nucleus

Molecular Function: protein serine/threonine kinase activity; protein binding; protein homodimerization activity; protein heterodimerization activity; ATP binding

Biological Process: I-kappaB kinase/NF-kappaB cascade; activation of MAPK activity; MyD88-independent toll-like receptor signaling pathway; stress-activated MAPK cascade; toll-like receptor 3 signaling pathway; protein amino acid phosphorylation; toll-like receptor 2 signaling pathway; activation of NF-kappaB transcription factor; toll-like receptor 10 signaling pathway; toll-like receptor 5 signaling pathway; MyD88-dependent toll-like receptor signaling pathway; inhibition of NF-kappaB transcription factor; lipopolysaccharide-mediated signaling pathway; toll-like receptor signaling pathway; regulation of cytokine and chemokine mediated signaling pathway; JNK cascade; innate immune response; toll-like receptor 9 signaling pathway; inflammatory response; toll-like receptor 4 signaling pathway