Rabbit IRAK-M Polyclonal Antibody | anti-IRAK-M antibody

IRAK-M Antibody

Cat. Num. AAA9407217

• Gene Names: IRAK3; ASRT5; IRAKM
• Reactivity: Human, Mouse, Rat
• Applications: ELISA, Western Blot, Immunohistochemistry
• Purity: Affinity chromatography purified via peptide column
• Synonyms: IRAK-M; Polyclonal Antibody; IRAK-M Antibody; anti-IRAK-M antibody

• Host: Rabbit
• Reactivity: Human, Mouse, Rat
• Clonality: Polyclonal
• Specificity: IRAK-M antibody has no cross response to IRAK or IRAK2.
• Purity/Purification: Affinity chromatography purified via peptide column
• Form/Format: Supplied in PBS containing 0.02% sodium azide.
• Sequence Length: 2288

• Applicable Applications for anti-IRAK-M antibody: ELISA (EIA), Western Blot (WB), Immunohistochemistry (IHC)
• Immunogen Type: Peptide
• Immunogen Description: Raised against a peptide corresponding to amino acids from the carboxy terminus of human IRAK-M.
• Target Name: IRAK-M
• Preparation and Storage: Can be stored at -20 degree C, stable for one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures.

Western Blot (WB)

(Western blot analysis of IRAK-M in mouse spleen (M) and rat liver (R) tissue lysates with IRAK-M antibody at 1 ug/mL.)

Immunohistochemistry (IHC)

(Immunohistochemical staining of rat liver cells using IRAK-M antibody at 2 ug/mL.)

Related Product Information for anti-IRAK-M antibody

Interleukin-1 (IL-1) and lipopolysaccharide (LPS) induces cellular response through IL-1 receptor (IL-1R) and Toll like receptors (TLR). IL-1 receptor-associated kinase (IRAK and IRAK2) mediates the activation of NF-betaB by IL-1/Toll receptors, which is a pivotal transcription factor mediating inflammatory and immune response. A novel member in the IRAK/Pelle family was recently identified and designated IRAK-M. IRAKs associate with IL-1/Toll receptors after IL-1 or LPS stimulation and the dominant negative mutants of IRAKs inhibit IL-1 or LPS induced NF-betaB activation. Members in IRAK/Pelle family play a central role in IL-1R/TLR mediated inflammatory responses to cytokine IL-1 and LPS.

Product Categories/Family for anti-IRAK-M antibody

Total protein Ab

NCBI and Uniprot Product Information

• NCBI GI #: 5225377
• NCBI GeneID: 11213
• Molecular Weight: 60,608 Da
• NCBI Official Full Name: Homo sapiens IL-1 receptor-associated-kinase-M mRNA, complete cds
• NCBI Official Synonym Full Names: interleukin-1 receptor-associated kinase 3
• NCBI Official Symbol: IRAK3
• NCBI Official Synonym Symbols: ASRT5; IRAKM
• NCBI Protein Information: interleukin-1 receptor-associated kinase 3
• UniProt Protein Name: Interleukin-1 receptor-associated kinase 3
• UniProt Gene Name: IRAK3
• UniProt Synonym Gene Names: IRAK-3; IRAK-M
• UniProt Entry Name: IRAK3_HUMAN

NCBI Description

This gene encodes a member of the interleukin-1 receptor-associated kinase protein family. Members of this family are essential components of the Toll/IL-R immune signal transduction pathways. This protein is primarily expressed in monocytes and macrophages and functions as a negative regulator of Toll-like receptor signaling. Mutations in this gene are associated with a susceptibility to asthma. Alternate splicing results in multiple transcript variants. [provided by RefSeq, May 2010]

Uniprot Description

IRAK3: a TKL kinase of the IRAK family. The interleukin-1 receptor-associated kinases are important mediators in the signal transduction of Toll-like receptor and IL1R family members, collectively referred to as TIRs.

Protein type: Kinase, protein; Protein kinase, Ser/Thr (non-receptor); Protein kinase, TKL; EC 2.7.11.1; TKL group; IRAK family

Chromosomal Location of Human Ortholog: 12q14.3

Cellular Component: cytoplasm; nucleus

Molecular Function: ATP binding; magnesium ion binding; protein heterodimerization activity; protein homodimerization activity; protein serine/threonine kinase activity

Biological Process: activation of NF-kappaB transcription factor; cytokine and chemokine mediated signaling pathway; inhibition of NF-kappaB transcription factor; MyD88-dependent toll-like receptor signaling pathway; negative regulation of cytokine and chemokine mediated signaling pathway; negative regulation of innate immune response; negative regulation of interleukin-12 production; negative regulation of interleukin-6 production; negative regulation of MAP kinase activity; negative regulation of protein catabolic process; negative regulation of protein complex disassembly; negative regulation of toll-like receptor signaling pathway; negative regulation of tumor necrosis factor production; protein amino acid autophosphorylation; protein amino acid phosphorylation; regulation of protein complex disassembly; response to exogenous dsRNA; response to lipopolysaccharide; response to peptidoglycan; response to virus

Disease: Asthma-related Traits, Susceptibility To, 5