Mouse ATP Binding Cassette Transporter G2 (ABCG2) ELISA Kit | ABCG2 elisa kit

Mouse ATP Binding Cassette Transporter G2 (ABCG2) ELISA Kit

Cat. Num. AAA2702755

• Gene Names: ABCG2; MRX; MXR; ABCP; BCRP; BMDP; MXR1; ABC15; BCRP1; CD338; GOUT1; MXR-1; CDw338; UAQTL1; EST157481
• Reactivity: Mouse
• Synonyms: ATP Binding Cassette Transporter G2 (ABCG2); Mouse ATP Binding Cassette Transporter G2 (ABCG2) ELISA Kit; CDw338; CD338; ABC-G2; MRX; BCRP1; ABC15; ABCP; BCRP; BMDP; MXR; MXR1; Mitoxantrone resistance-associated protein; Breast Cancer Resistance Protein 1; Urate exporter; ABCG2 elisa kit

• Reactivity: Mouse
• Specificity: This assay has high sensitivity and excellent specificity for detection of ATP Binding Cassette Transporter G2 (ABCG2).; No significant cross-reactivity or interference between ATP Binding Cassette Transporter G2 (ABCG2) and analogues was observed.
• Sequence Length: 611

• Assay Type: Double-antibody Sandwich
• Samples: Tissue Homogenates and Other Biological Fluids
• Detection Range: 0.156-10ng/mL
• Sensitivity: < 0.055ng/mL
• Application: Enzyme-linked immunosorbent assay for Antigen Detection.
• Intra-assay Precision (Precision within an assay): 3 samples with low, middle and high level ATP Binding Cassette Transporter G2 (ABCG2) were tested 20 times on one plate, respectively.
• Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level ATP Binding Cassette Transporter G2 (ABCG2) were tested on 3 different plates, 8 replicates in each plate.
• CV(%) =: SD/meanX100
• Intra-Assay: CV<10%
• Inter-Assay: CV<12%
• Preparation and Storage: The stability of kit is determined by the loss rate of activity. The loss rate of this kit is less than 5% within the expiration date under appropriate storage condition.; To minimize extra influence on the performance, operation procedures and lab conditions, especially room temperature, air humidity, incubator temperature should be strictly controlled. It is also strongly suggested that the whole assay is performed by the same operator from the beginning to the end.

Typical Testing Data/Standard Curve (for reference only)

Related Product Information for ABCG2 elisa kit

The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to ATP Binding Cassette Transporter G2 (ABCG2). Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody specific to ATP Binding Cassette Transporter G2 (ABCG2). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain ATP Binding Cassette Transporter G2 (ABCG2), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of ATP Binding Cassette Transporter G2 (ABCG2) in the samples is then determined by comparing the O.D. of the samples to the standard curve.

NCBI and Uniprot Product Information

• NCBI GI #: 383792176
• NCBI GeneID: 9429
• NCBI Accession #: NP_001244315.1
• NCBI GenBank Nucleotide #: NM_001257386.2
• UniProt Accession #: Q9UNQ0; O95374; Q4W5I3; Q53ZQ1; Q569L4; Q5YLG4; Q86V64; Q8IX16; Q96LD6; Q96TA8; A0A1W3; A8K1T5
• Molecular Weight: 67,453 Da
• NCBI Official Full Name: ATP-binding cassette sub-family G member 2 isoform 2
• NCBI Official Synonym Full Names: ATP binding cassette subfamily G member 2 (Junior blood group)
• NCBI Official Symbol: ABCG2
• NCBI Official Synonym Symbols: MRX; MXR; ABCP; BCRP; BMDP; MXR1; ABC15; BCRP1; CD338; GOUT1; MXR-1; CDw338; UAQTL1; EST157481
• NCBI Protein Information: ATP-binding cassette sub-family G member 2
• UniProt Protein Name: ATP-binding cassette sub-family G member 2
• Protein Family: ABC transporter G family
• UniProt Gene Name: ABCG2
• UniProt Synonym Gene Names: ABCP; BCRP; BCRP1; MXR

NCBI Description

The membrane-associated protein encoded by this gene is included in the superfamily of ATP-binding cassette (ABC) transporters. ABC proteins transport various molecules across extra- and intra-cellular membranes. ABC genes are divided into seven distinct subfamilies (ABC1, MDR/TAP, MRP, ALD, OABP, GCN20, White). This protein is a member of the White subfamily. Alternatively referred to as a breast cancer resistance protein, this protein functions as a xenobiotic transporter which may play a major role in multi-drug resistance. It likely serves as a cellular defense mechanism in response to mitoxantrone and anthracycline exposure. Significant expression of this protein has been observed in the placenta, which may suggest a potential role for this molecule in placenta tissue. Multiple transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, Apr 2012]

Uniprot Description

ABCG2: Xenobiotic transporter that may play an important role in the exclusion of xenobiotics from the brain. May be involved in brain-to-blood efflux. Appears to play a major role in the multidrug resistance phenotype of several cancer cell lines. When overexpressed, the transfected cells become resistant to mitoxantrone, daunorubicin and doxorubicin, display diminished intracellular accumulation of daunorubicin, and manifest an ATP- dependent increase in the efflux of rhodamine 123. Monomer or homodimer; disulfide-linked. Up-regulated in brain tumors. Highly expressed in placenta. Low expression in small intestine, liver and colon. Belongs to the ABC transporter superfamily. ABCG family. Eye pigment precursor importer (TC 3.A.1.204) subfamily. 2 isoforms of the human protein are produced by alternative splicing.

Protein type: Membrane protein, integral; Membrane protein, multi-pass; Transporter; Transporter, ABC family; Transporter, iron

Chromosomal Location of Human Ortholog: 4q22.1

Cellular Component: apical plasma membrane; integral component of membrane; mitochondrial membrane; nucleus; plasma membrane; receptor complex

Molecular Function: ATP binding; ATPase activity, coupled to transmembrane movement of substances; cholesterol transporter activity; heme transporter activity; protein binding; protein homodimerization activity; transporter activity; xenobiotic transmembrane transporting ATPase activity

Biological Process: cellular iron ion homeostasis; cholesterol efflux; drug transmembrane transport; heme transport; response to drug; transport; urate metabolic process; xenobiotic transport

Disease: Blood Group, Junior System; Uric Acid Concentration, Serum, Quantitative Trait Locus 1