Rabbit IRAK-M Polyclonal Antibody | anti-IRAK3 antibody

IRAK-M Antibody

Cat. Num. AAA150500

• Gene Names: IRAK3; ASRT5; IRAKM
• Reactivity: Human, Mouse, Rat
• Applications: ELISA, Western Blot, Immunohistochemistry, Immunofluorescence
• Purity: IRAK-M Antibody is affinity chromatography purified via peptide column.
• Synonyms: IRAK-M; Polyclonal Antibody; IRAK-M Antibody; ASRT5; IRAKM; Interleukin-1 receptor-associated kinase 3; IL-1 receptor-associated kinase M; IRAK-3; interleukin-1 receptor-associated kinase 3; anti-IRAK3 antibody

• Host: Rabbit
• Reactivity: Human, Mouse, Rat
• Clonality: Polyclonal
• Isotype: IgG
• Specificity: IRAK-M antibody has no cross response to IRAK or IRAK2.
• Purity/Purification: IRAK-M Antibody is affinity chromatography purified via peptide column.
• Form/Format: Liquid
• Sequence Length: 2288

• Applicable Applications for anti-IRAK3 antibody: ELISA (EIA), Western Blot (WB), Immunohistochemistry (IHC), Immunofluorescence (IF)
• Application Notes: IRAK-M antibody can be used for detection of IRAK-M by Western blot at 1 mug/mL. A 68 kDa major band can be detected. IRAK-M antibody can also detect IRAK-M by immunohistochemistry at 2 mug/mL. For immunofluorescence start at 10 mug/mL.
• Conjugate: Unconjugated
• Immunogen: IRAK-M antibody was raised against a peptide corresponding to amino acids from the carboxy terminus of human IRAK-M.
• Buffer: IRAK-M Antibody is supplied in PBS containing 0.02% sodium azide.
• Preparation and Storage: IRAK-M antibody can be stored at 4 degree C for three months and -20 degree C, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures.

Western Blot (WB)

(Western blot analysis of IRAK-M in (M) mouse spleen and (R) rat liver tissue lysates with IRAK-M antibody at 1 μg/mL.)

Immunohistochemistry (IHC)

(Immunohistochemistry of IRAK-M in human spleen tissue with IRAK-M antibody at 5 μg/mL.)

Immunofluorescence (IF)

(Immunofluorescence of IRAK-M in Rat Liver tissue with IRAK-M antibody at 10 μg/mL.)

Immunohistochemistry (IHC)

(Immunohistochemical staining of rat liver tissue using IRAK-M antibody at 2 μg/mL.)

Immunofluorescence (IF)

(Immunofluorescence of IRAK2 in A20 cells with IRAK2 antibody at 20 μg/mL.Red: IRAK-M Antibody (2355)Blue: DAPI staining)

Related Product Information for anti-IRAK3 antibody

IRAK-M Antibody: Interleukin-1 (IL-1) and lipopolysaccharide (LPS) induces cellular response through IL-1 receptor (IL-1R) and Toll like receptors (TLR). IL-1 receptor-associated kinase (IRAK and IRAK2) mediates the activation of NF-betaB by IL-1/Toll receptors, which is a pivotal transcription factor mediating inflammatory and immune response. A novel member in the IRAK/Pelle family was recently identified and designated IRAK-M. IRAKs associate with IL-1/Toll receptors after IL-1 or LPS stimulation and the dominant negative mutants of IRAKs inhibit IL-1 or LPS induced NF-betaB activation. Members in IRAK/Pelle family play a central role in IL-1R/TLR mediated inflammatory responses to cytokine IL-1 and LPS.

NCBI and Uniprot Product Information

• NCBI GI #: 5225377
• NCBI GeneID: 11213
• UniProt Accession #: Q9Y616; B4DQ57
• Molecular Weight: 68 kDa
• NCBI Official Full Name: Homo sapiens IL-1 receptor-associated-kinase-M mRNA, complete cds
• NCBI Official Synonym Full Names: interleukin-1 receptor-associated kinase 3
• NCBI Official Symbol: IRAK3
• NCBI Official Synonym Symbols: ASRT5; IRAKM
• NCBI Protein Information: interleukin-1 receptor-associated kinase 3; IRAK3; IL-1 receptor-associated kinase M
• UniProt Protein Name: Interleukin-1 receptor-associated kinase 3
• Protein Family: Interleukin-1 receptor-associated kinase
• UniProt Gene Name: IRAK3
• UniProt Synonym Gene Names: IRAK-3; IRAK-M
• UniProt Entry Name: IRAK3_HUMAN

NCBI Description

This gene encodes a member of the interleukin-1 receptor-associated kinase protein family. Members of this family are essential components of the Toll/IL-R immune signal transduction pathways. This protein is primarily expressed in monocytes and macrophages and functions as a negative regulator of Toll-like receptor signaling. Mutations in this gene are associated with a susceptibility to asthma. Alternate splicing results in multiple transcript variants. [provided by RefSeq, May 2010]

Uniprot Description

IRAK3: a TKL kinase of the IRAK family. The interleukin-1 receptor-associated kinases are important mediators in the signal transduction of Toll-like receptor and IL1R family members, collectively referred to as TIRs.

Protein type: Protein kinase, Ser/Thr (non-receptor); Kinase, protein; Protein kinase, TKL; EC 2.7.11.1; TKL group; IRAK family

Chromosomal Location of Human Ortholog: 12q14.3

Cellular Component: interleukin-1 receptor complex; cytoplasm; nucleus

Molecular Function: protein serine/threonine kinase activity; protein homodimerization activity; protein heterodimerization activity; magnesium ion binding; ATP binding

Biological Process: negative regulation of MAP kinase activity; regulation of protein complex disassembly; negative regulation of interleukin-12 production; protein amino acid autophosphorylation; cytokine and chemokine mediated signaling pathway; response to virus; response to lipopolysaccharide; negative regulation of tumor necrosis factor production; protein amino acid phosphorylation; activation of NF-kappaB transcription factor; MyD88-dependent toll-like receptor signaling pathway; response to exogenous dsRNA; negative regulation of innate immune response; inhibition of NF-kappaB transcription factor; response to peptidoglycan; negative regulation of toll-like receptor signaling pathway; toll-like receptor signaling pathway; negative regulation of interleukin-6 production; negative regulation of cytokine and chemokine mediated signaling pathway; negative regulation of protein catabolic process; negative regulation of protein complex disassembly

Disease: Asthma-related Traits, Susceptibility To, 5