Western Blot (WB)
(Western Blot analysis of Hela cells with IRAK-1 Polyclonal Antibody.)
IRAK Isoform Antibody Kit | IRAK antibody kit
IRAK Isoform Antibody Sampler Kit
Applications
Western Blot
Synonyms
IRAK Isoform; IRAK Isoform Antibody Sampler Kit; IRAK antibody kit
Applicable Applications for IRAK antibody kit
Western Blot (WB)
Western Blot (WB)
(Western Blot analysis of Hela cells with IRAK-1 Polyclonal Antibody.)
Western Blot (WB)
(Western Blot analysis of Hela cells with IRAK-1 Polyclonal Antibody.)
Western Blot (WB)
(Western Blot analysis of Jurkat, HuvEc cells using IRAK-2 Polyclonal Antibody at dilution of 1:2000.)
Western Blot (WB)
(Western Blot analysis of Jurkat, HuvEc cells using IRAK-2 Polyclonal Antibody at dilution of 1:2000.)
Western Blot (WB)
(Western Blot analysis of LOVO cells with IRAK-M Polyclonal Antibody.)
Western Blot (WB)
(Western Blot analysis of LOVO cells with IRAK-M Polyclonal Antibody.)
Related Product Information for IRAK antibody kit
Background: Interleukin-1 (IL-1) receptor-associated kinase (IRAK) is a serine/threonine-specific kinase that can be coprecipitated in an IL-1-inducible manner with the IL-1 receptor. The mammalian family of IRAK molecules contains four members (IRAK1, IRAK2, IRAK3/IRAK- M, and IRAK4). The binding of IL-1 to IL-1 receptor type I (IL-1RI) initiates the formation of a complex that includes IL-1RI, AcP, MyD88, and IRAKs. IRAK undergoes autophosphorylation shortly after IL-1 stimulation. The subsequent events involve IRAK dissociation from the IL-1RI complex, its ubiquitination, and its association with two membrane-bound proteins: TAB2 and TRAF6. The resulting IRAK- TRAF6-TAB2 complex is then released into the cytoplasm where it activates protein kinase cascades, including TAK1, IKKs, and the stress- activated kinases. Upon IL-1R/TLR (Toll-Like Receptor) ligation, IRAK1 and IRAK4 are rapidly recruited to the receptor by the adaptor MyD88. IRAK1 is phosphorylated by IRAK4 at Thr209 and Thr387, followed by sequential autohyperphosphorylation in various domains. Unlike IRAK1 and IRAK4, IRAK2 and IRAK-M do not have significant kinase activity although they can still activate NF-kappaB when overexpressed. Antisense oligonucleotide depletion of IRAK2 can inhibit IL-1 mediated NF-kappaB activation. Expression of IRAK-M is more restricted compared to other family members with highest levels of expression occurring in monocytes/macrophages. Studies from IRAK-M knockout mice suggest that it may play a role as a negative regulator of TLR signaling and innate immune responses by preventing the dissociation of IRAK1 and IRAK4 from MyD88 and the subsequent formation of its complex with TRAF6.
Similar Products
Product Notes
The IRAK (Catalog #AAA2550056) is an Antibody Kit and is intended for research purposes only. The product is available for immediate purchase. AAA Biotech's IRAK Isoform can be used in a range of immunoassay formats including, but not limited to, Western Blot (WB). Researchers should empirically determine the suitability of the IRAK for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "IRAK Isoform, Antibody Kit" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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