Rabbit anti-Human IRAK-M Polyclonal Antibody | anti-IRAK-M antibody

IRAK-M (L519) Polyclonal Antibody

Cat. Num. AAA3003016

• Gene Names: IRAK3; ASRT5; IRAKM
• Reactivity: Human
• Applications: Immunohistochemistry, Immunofluorescence
• Purity: The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen and the purity is >95% (by SDS-PAGE).
• Synonyms: IRAK-M; Polyclonal Antibody; IRAK-M (L519) Polyclonal Antibody; Interleukin-1 receptor-associated kinase 3; IRAK-3; IL-1 receptor-associated kinase M; IRAKM; IRAK3; anti-IRAK-M antibody

• Host: Rabbit
• Reactivity: Human
• Clonality: Polyclonal
• Isotype: IgG
• Specificity: IRAK-M (L519) polyclonal antibody detects endogenous levels of IRAK-M protein.
• Purity/Purification: The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen and the purity is >95% (by SDS-PAGE).
• Form/Format: Rabbit IgG, 1mg/ml in PBS with 0.02% sodium azide, 50% glycerol, pH7.2
• Sequence Length: 535

• Applicable Applications for anti-IRAK-M antibody: Immunohistochemistry (IHC), Immunofluorescence (IF)
• Application Notes: IHC: 1:50-1:200; IF: 1:50-1:200
• Immunogen: Synthetic peptide, corresponding to amino acids 500-540 of Human IRAK-M.
• Preparation and Storage: Store at 4 degree C short term.; Aliquot and store at -20 degree C long term.; Avoid freeze-thaw cycles.

Immunohistochemistry (IHC)

(Immunohistochemistry (IHC) analyzes of IRAK-M (L519) pAb in paraffin-embedded human brain tissue.)

Related Product Information for anti-IRAK-M antibody

Interleukin-1 receptor (IL1R)-associated kinases (IRAKs) are important mediators in the signal transduction of Toll-like receptor (TLR) and IL1R family members, collectively referred to as TIRs. Binding of IL-1 to its cognate receptor results in the activation of the NFkappaB signaling pathway. A 100 kDa IL-1- dependent kinase termed IRAK-1 (for IL-1 receptor- associated kinase 1) coimmunoprecipitates with activated IL-1RI and is implicated as an upstream mediator of NFkappaB activation. A related Drosophila protein, Pelle, is a known upstream activator of Dorsal, the Drosophila homolog of NFkappaB. IRAK-2 is a proximal mediator of IL1, a component of the IL1R signaling complex, and is required for IL1R-induced NFkappaB activation. IRAK-4, like IRAK-1 and Pelle, has auto- and cross-phosphorylation kinase activity. IRAK-4 is strongly expressed in kidney and is also found in lung, testis, small intestine, breast, liver, and placenta. In contrast to the other IRAKs that are expressed in most cell types, IRAK-M is restricted to monocytic cells. IRAK-M mRNA transcripts are found predominantly in PBL and the monocytic cell lines U937 and THP-1.

NCBI and Uniprot Product Information

• NCBI GI #: 216547504
• NCBI GeneID: 11213
• NCBI Accession #: NP_001135995.1
• NCBI GenBank Nucleotide #: NP_001135995.1
• UniProt Accession #: Q9Y616
• Molecular Weight: ~ 68kDa
• NCBI Official Full Name: interleukin-1 receptor-associated kinase 3 isoform b
• NCBI Official Synonym Full Names: interleukin 1 receptor associated kinase 3
• NCBI Official Symbol: IRAK3
• NCBI Official Synonym Symbols: ASRT5; IRAKM
• NCBI Protein Information: interleukin-1 receptor-associated kinase 3
• UniProt Protein Name: Interleukin-1 receptor-associated kinase 3
• UniProt Gene Name: IRAK3
• UniProt Synonym Gene Names: IRAK-3; IRAK-M
• UniProt Entry Name: IRAK3_HUMAN

NCBI Description

This gene encodes a member of the interleukin-1 receptor-associated kinase protein family. Members of this family are essential components of the Toll/IL-R immune signal transduction pathways. This protein is primarily expressed in monocytes and macrophages and functions as a negative regulator of Toll-like receptor signaling. Mutations in this gene are associated with a susceptibility to asthma. Alternate splicing results in multiple transcript variants. [provided by RefSeq, May 2010]

Uniprot Description

IRAK3: a TKL kinase of the IRAK family. The interleukin-1 receptor-associated kinases are important mediators in the signal transduction of Toll-like receptor and IL1R family members, collectively referred to as TIRs.

Protein type: Protein kinase, TKL; Kinase, protein; Protein kinase, Ser/Thr (non-receptor); EC 2.7.11.1; TKL group; IRAK family

Chromosomal Location of Human Ortholog: 12q14.3

Cellular Component: interleukin-1 receptor complex; cytoplasm; nucleus

Molecular Function: protein serine/threonine kinase activity; protein homodimerization activity; protein heterodimerization activity; magnesium ion binding; ATP binding

Biological Process: negative regulation of MAP kinase activity; regulation of protein complex disassembly; negative regulation of interleukin-12 production; cytokine and chemokine mediated signaling pathway; response to virus; protein amino acid autophosphorylation; response to lipopolysaccharide; negative regulation of tumor necrosis factor production; protein amino acid phosphorylation; activation of NF-kappaB transcription factor; MyD88-dependent toll-like receptor signaling pathway; response to exogenous dsRNA; negative regulation of innate immune response; inhibition of NF-kappaB transcription factor; response to peptidoglycan; toll-like receptor signaling pathway; negative regulation of interleukin-6 production; negative regulation of toll-like receptor signaling pathway; negative regulation of cytokine and chemokine mediated signaling pathway; negative regulation of protein catabolic process; negative regulation of protein complex disassembly

Disease: Asthma-related Traits, Susceptibility To, 5