Rabbit anti-Human Indoleamine-2,3-Dioxygenase (IDO) Polyclonal Antibody | anti-IDO antibody
FITC-linked Antibody to Indoleamine-2,3-Dioxygenase (IDO)
Reactivity
Human
Applications
Immunohistochemistry, Immunocytochemistry, Immunofluorescence, Western Blot
Purity
Affinity Chromatography
Synonyms
Indoleamine-2; 3-Dioxygenase (IDO); Polyclonal Antibody; FITC-linked Antibody to Indoleamine-2; anti-IDO antibody
Host
Rabbit
Reactivity
Human
Clonality
Polyclonal
Specificity
The antibody is a rabbit polyclonal antibody raised against IDO conjugated to fitc. It has been selected for its ability to recognize IDO in immunohistochemical staining andwestern blotting.
Purity/Purification
Affinity Chromatography
Form/Format
Supplied as solution form in PBS, pH7.4, containing 0.02% NaN3,50% glycerol.
Concentration
500ug/ml (varies by lot)
Sequence Length
453
Applicable Applications for anti-IDO antibody
Immunohistochemistry (IHC), Immunocytochemistry (ICC), Immunofluorescence (IF), Western Blot (WB)
Application Notes
Western blotting: 1:100-400
Immunocytochemistry in formalin fixed cells: 1:100-500
Immunohistochemistry in formalin fixed frozen section: 1:100-500
Immunohistochemistry in paraffin section: 1:50-200
Enzyme-linked Immunosorbent Assay: 1:100-200
Immunocytochemistry in formalin fixed cells: 1:100-500
Immunohistochemistry in formalin fixed frozen section: 1:100-500
Immunohistochemistry in paraffin section: 1:50-200
Enzyme-linked Immunosorbent Assay: 1:100-200
Preparation and Storage
Store at 4 degree C for frequent use. Stored at -20 degree C to -80 degree C in a manual defrost freezer for one year without detectable loss of activity. Avoid repeated freeze-thaw cycles.
Product Categories/Family for anti-IDO antibody
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
Molecular Weight
50,775 Da
NCBI Official Full Name
Indoleamine 2,3-dioxygenase
NCBI Official Symbol
BNA2
NCBI Protein Information
dioxygenase BNA2
UniProt Protein Name
Indoleamine 2,3-dioxygenase
Protein Family
UniProt Gene Name
BNA2
UniProt Synonym Gene Names
IDO
UniProt Entry Name
I23O_YEAST
Uniprot Description
Function: Catalyzes the first step in tryptophan catabolism in order to supply de novo nicotinamide adenine dinucleotide (NAD+) via the kynurenine pathway. Plays a role in the cellular response to telomere uncapping. Ref.4 Ref.6 Ref.8 Ref.9
Catalytic activity: D-tryptophan + O2 = N-formyl-D-kynurenine. Ref.9L-tryptophan + O2 = N-formyl-L-kynurenine. Ref.9
Cofactor: Binds 1 heme group per subunit
By similarity.
Pathway: Cofactor biosynthesis; NAD(+) biosynthesis. Ref.4
Induction: Expression is mediated by the AFT2, HCM1, and SUM1 transcription factors. Up-regulated in absence of NPT1. Ref.5 Ref.6 Ref.10
Miscellaneous: Present with 149 molecules/cell in log phase SD medium.
Sequence similarities: Belongs to the indoleamine 2,3-dioxygenase family.
Biophysicochemical propertiesKinetic parameters:KM=412 µM for L-tryptophan Ref.9KM=9.2 mM for D-tryptophan
Research Articles on IDO
Similar Products
Product Notes
The IDO bna2 (Catalog #AAA2013461) is an Antibody produced from Rabbit and is intended for research purposes only. The product is available for immediate purchase. The FITC-linked Antibody to Indoleamine-2,3-Dioxygenase (IDO) reacts with Human and may cross-react with other species as described in the data sheet. AAA Biotech's Indoleamine-2,3-Dioxygenase (IDO) can be used in a range of immunoassay formats including, but not limited to, Immunohistochemistry (IHC), Immunocytochemistry (ICC), Immunofluorescence (IF), Western Blot (WB). Western blotting: 1:100-400 Immunocytochemistry in formalin fixed cells: 1:100-500 Immunohistochemistry in formalin fixed frozen section: 1:100-500 Immunohistochemistry in paraffin section: 1:50-200 Enzyme-linked Immunosorbent Assay: 1:100-200. Researchers should empirically determine the suitability of the IDO bna2 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "Indoleamine-2,3-Dioxygenase (IDO), Polyclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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