Rabbit PINK1 Polyclonal Antibody | anti-PINK1 antibody

PINK1 antibody - N-terminal region

Cat. Num. AAA3215961

• Gene Names: PINK1; BRPK; PARK6
• Reactivity: Cow, Guinea Pig, Horse, Human, Mouse, Rabbit, Rat
• Applications: Western Blot, Immunohistochemistry
• Purity: Affinity Purified
• Synonyms: PINK1; Polyclonal Antibody; PINK1 antibody - N-terminal region; anti-PINK1 antibody

• Host: Rabbit
• Reactivity: Cow, Guinea Pig, Horse, Human, Mouse, Rabbit, Rat
• Clonality: Polyclonal
• Purity/Purification: Affinity Purified
• Form/Format: Liquid. Purified antibody supplied in 1x PBS buffer with 0.09% (w/v) sodium azide and 2% sucrose.
• Concentration: Batch dependent within range: 100 ul at 0.5- 1mg/ml (varies by lot)
• Sequence: Synthetic peptide located within the following region: GCAGPCGRAVFLAFGLGLGLIEEKQAESRRAVSACQEIQAIFTQKSKPGP
• Sequence Length: 581

• Applicable Applications for anti-PINK1 antibody: Western Blot (WB), Immunohistochemistry (IHC)
• Homology: Cow: 93%; Guinea Pig: 100%; Horse: 93%; Human: 100%; Mouse: 93%; Rabbit: 100%; Rat: 100%
• Protein Size: 581 amino acids
• Protein Interactions: MMT; RHOT2; MCL1; BCL2L2; BCL2L1; UBC; BAG2; CRLS1; FBXO7; SARM1; RB1CC1; TOMM20; VIM; TRAF6; HSPA1B; HSPA1A; PARK2; HSP90AA1; TOLLIP; MAP3K7; IRAK1; RICTOR; SENP8; MAPKAP1; COPS8; UBE2M; AKT1; NEDD8; PINK1; TOMM22; RHOT1; HTRA2; PARK7; CDC37; TOMM40; TO
• REplacement Item: This antibody may replace item sc-32583 from Santa Cruz Biotechnology.
• Preparation and Storage: For short term use, store at 2-8 degree C up to 1 week. For long term storage, store at -20 degree C in small aliquots to prevent freeze-thaw cycles.

Immunohistochemistry (IHC)

(Rabbit Anti-PINK1 AntibodyFormalin Fixed Paraffin Embedded Tissue: Human heart TissueObserved Staining: CytoplasmicPrimary Antibody Concentration: N/AOther Working Concentrations: 1:600Secondary Antibody: Donkey anti-Rabbit-Cy3Secondary Antibody Concentration: 1:200Magnification: 20XExposure Time: 0.5 - 2.0 sec)

Immunohistochemistry (IHC)

(Rabbit Anti-PINK1 AntibodyFormalin Fixed Paraffin Embedded Tissue: Human Liver TissueObserved Staining: Cytoplasm in hepatocytesPrimary Antibody Concentration: 1:100Other Working Concentrations: N/ASecondary Antibody: Donkey anti-Rabbit-Cy3Secondary Antibody Concentration: 1:200Magnification: 20XExposure Time: 0.5 - 2.0 sec)

Western Blot (WB)

(WB Suggested Anti-PINK1 AntibodyTitration: 1.0 ug/mlPositive Control: RPMI-8226 Whole Cell)

Related Product Information for anti-PINK1 antibody

This is a rabbit polyclonal antibody against PINK1. It was validated on Western Blot

Target Description: This gene encodes a serine/threonine protein kinase that localizes to mitochondria. It is thought to protect cells from stress-induced mitochondrial dysfunction. Mutations in this gene cause one form of autosomal recessive early-onset Parkinson disease.

Product Categories/Family for anti-PINK1 antibody

Polyclonal; Mitochondria; Neuroscience; Phosphorylation

NCBI and Uniprot Product Information

• NCBI GI #: 14165272
• NCBI GeneID: 65018
• NCBI Accession #: NP_115785
• NCBI GenBank Nucleotide #: NM_032409
• UniProt Accession #: Q9BXM7
• Molecular Weight: 63kDa
• NCBI Official Full Name: serine/threonine-protein kinase PINK1, mitochondrial
• NCBI Official Synonym Full Names: PTEN induced kinase 1
• NCBI Official Symbol: PINK1
• NCBI Official Synonym Symbols: BRPK; PARK6
• NCBI Protein Information: serine/threonine-protein kinase PINK1, mitochondrial
• UniProt Protein Name: Serine/threonine-protein kinase PINK1, mitochondrial
• Protein Family: Serine/threonine-protein kinase
• UniProt Gene Name: PINK1
• UniProt Entry Name: PINK1_HUMAN

NCBI Description

This gene encodes a serine/threonine protein kinase that localizes to mitochondria. It is thought to protect cells from stress-induced mitochondrial dysfunction. Mutations in this gene cause one form of autosomal recessive early-onset Parkinson disease. [provided by RefSeq, Jul 2008]

Uniprot Description

PINK1: Protects against mitochondrial dysfunction during cellular stress, potentially by phosphorylating mitochondrial proteins. Involved in the clearance of damaged mitochondria via selective autophagy (mitophagy). It is necessary for PARK2 recruitment to dysfunctional mitochondria to initiate their degradation. Interats with PARK2. Highly expressed in heart, skeletal muscle and testis, and at lower levels in brain, placenta, liver, kidney, pancreas, prostate, ovary and small intestine. Present in the embryonic testis from an early stage of development. Belongs to the protein kinase superfamily. Ser/Thr protein kinase family. 2 isoforms of the human protein are produced by alternative splicing.

Protein type: Kinase, protein; Membrane protein, integral; Protein kinase, Ser/Thr (non-receptor); Mitochondrial; EC 2.7.11.1; Protein kinase, Other; Other group; NKF2 family

Chromosomal Location of Human Ortholog: 1p36

Cellular Component: mitochondrion; mitochondrial intermembrane space; cytosol; mitochondrial outer membrane; cytoskeleton; membrane; axon; perinuclear region of cytoplasm; cytoplasm; mitochondrial inner membrane; TORC2 complex; chromatin; nucleus; integral to mitochondrial outer membrane

Molecular Function: protein kinase B binding; protein serine/threonine kinase activity; protein binding; protease activator activity; protease binding; ubiquitin protein ligase binding; magnesium ion binding; kinase activity; ATP binding

Biological Process: ubiquitin-dependent protein catabolic process; negative regulation of JNK cascade; positive regulation of dopamine secretion; positive regulation of translation; regulation of protein ubiquitination; protein ubiquitination; protein amino acid phosphorylation; positive regulation of protein amino acid dephosphorylation; negative regulation of macroautophagy; regulation of mitochondrial membrane potential; positive regulation of ubiquitin-protein ligase activity; response to stress; regulation of protein complex assembly; negative regulation of neuron apoptosis; regulation of hydrogen peroxide metabolic process; mitochondrion organization and biogenesis; positive regulation of I-kappaB kinase/NF-kappaB cascade; protein stabilization; activation of protein kinase B; positive regulation of synaptic transmission, dopaminergic; positive regulation of peptidyl-serine phosphorylation; positive regulation of protein kinase B signaling cascade; peptidyl-serine phosphorylation; mitochondrion degradation; positive regulation of transcription factor activity; positive regulation of protein amino acid phosphorylation; response to oxidative stress; phosphorylation

Disease: Parkinson Disease 6, Autosomal Recessive Early-onset