K-Ras / K-Ras Recombinant Protein | K-Ras recombinant protein

Recombinant Human K-Ras / K-Ras Protein (His tag)

Cat. Num. AAA2545532

• Gene Names: KRAS; NS; NS3; CFC2; RALD; KRAS1; KRAS2; RASK2; KI-RAS; C-K-RAS; K-RAS2A; K-RAS2B; K-RAS4A; K-RAS4B; c-Ki-ras2
• Purity: > 90 % as determined by SDS-PAGE
• Synonyms: K-Ras / K-Ras; Recombinant Human K-Ras / K-Ras Protein (His tag); C-K-RAS; K-RAS2A; K-RAS2B; K-RAS4A; K-RAS4B; KI-RAS; KRAS1; KRAS2; NS; NS3; RASK2; K-Ras recombinant protein

• Host: E Coli
• Purity/Purification: > 90 % as determined by SDS-PAGE
• Form/Format: Lyophilized from sterile 20mM Tris, 0.5M NaCl, pH 7.5
• Sequence Length: 188

• Application Notes: The recombinant human KRAS consisting of 195 amino acids and has a calculated molecular mass of 22.5 kDa. It migrates as an 25 kDa band in SDS-PAGE under reducing conditions as predicted
• Predicted N Terminal: Met
• Preparation and Storage: Samples are stable for up to twelve months from date of receipt at -70 degree C

SDS-PAGE

Related Product Information for K-Ras recombinant protein

Background: K-Ras belongs to the small GTPase superfamily, Ras family. As other members of the Ras family, K-Ras is a GTPase and is an early player in many signal transduction pathways. It is usually tethered to cell membranes because of the presence of an isoprenyl group on its C-terminus. K-Ras functions as a molecular on/off switch. Once it is turned on it recruits and activates proteins necessary for the propagation of growth factor and other receptors' signal, such as c-Raf and PI 3-kinase. It binds to GTP in the active state and possesses an intrinsic enzymatic activity which cleaves the terminal phosphate of the nucleotide converting it to GDP. Upon conversion of GTP to GDP, K-Ras is turned off. The rate of conversion is usually slow but can be sped up dramatically by an accessory protein of the GTPase activating protein class, for example RasGAP. In turn K-Ras can bind to proteins of the Guanine Nucleotide Exchange Factor class, for example SOS1, which forces the release of bound nucleotide. Subsequently, K-Ras binds GTP present in the cytosol and the GEF is released from ras-GTP. Besides essential function in normal tissue signaling, the mutation of a K-Ras gene is an essential step in the development of many cancers. Several germline K-Ras mutations have been found to be associated with Noonan syndrome[4] and cardio-facio-cutaneous syndrome. Somatic K-Ras mutations are found at high rates in Leukemias, colon cancer, pancreatic cancer and lung cancer.

Description: A DNA sequence encoding the native human KRAS (AAH13572.1) (Thr 2-Cys 185) was expressed, with a polyhistide tag at the N-terminus.

NCBI and Uniprot Product Information

• NCBI GI #: 15488883
• NCBI GeneID: 3845
• Molecular Weight: 21,425 Da
• NCBI Official Full Name: V-Ki-ras2 Kirsten rat sarcoma viral oncogene homolog
• NCBI Official Synonym Full Names: KRAS proto-oncogene, GTPase
• NCBI Official Symbol: KRAS
• NCBI Official Synonym Symbols: NS; NS3; CFC2; RALD; KRAS1; KRAS2; RASK2; KI-RAS; C-K-RAS; K-RAS2A; K-RAS2B; K-RAS4A; K-RAS4B; c-Ki-ras2
• NCBI Protein Information: GTPase KRas
• UniProt Protein Name: GTPase KRas
• UniProt Gene Name: KRAS
• UniProt Synonym Gene Names: KRAS2; RASK2

NCBI Description

This gene, a Kirsten ras oncogene homolog from the mammalian ras gene family, encodes a protein that is a member of the small GTPase superfamily. A single amino acid substitution is responsible for an activating mutation. The transforming protein that results is implicated in various malignancies, including lung adenocarcinoma, mucinous adenoma, ductal carcinoma of the pancreas and colorectal carcinoma. Alternative splicing leads to variants encoding two isoforms that differ in the C-terminal region. [provided by RefSeq, Jul 2008]

Uniprot Description

KRas: Ras proteins bind GDP/GTP and possess intrinsic GTPase activity. Interacts with PHLPP. Interacts (active GTP-bound form preferentially) with RGS14. Alternate between an inactive form bound to GDP and an active form bound to GTP. Activated by a guanine nucleotide-exchange factor (GEF) and inactivated by a GTPase- activating protein (GAP). Belongs to the small GTPase superfamily. Ras family. 2 isoforms of the human protein are produced by alternative splicing.

Protein type: G protein; G protein, monomeric; G protein, monomeric, Ras; Motility/polarity/chemotaxis; Oncoprotein

Chromosomal Location of Human Ortholog: 12p12.1

Cellular Component: cytoplasm; extrinsic to internal side of plasma membrane; focal adhesion; membrane; plasma membrane

Molecular Function: protein binding; protein complex binding

Biological Process: axon guidance; epidermal growth factor receptor signaling pathway; leukocyte migration; MAPKKK cascade; positive regulation of cell proliferation; positive regulation of protein amino acid phosphorylation; Ras protein signal transduction; regulation of protein stability; stimulatory C-type lectin receptor signaling pathway

Disease: Bladder Cancer; Breast Cancer; Cardiofaciocutaneous Syndrome 2; Gastric Cancer, Hereditary Diffuse; Leukemia, Acute Myeloid; Lung Cancer; Noonan Syndrome 3; Pancreatic Cancer; Schimmelpenning-feuerstein-mims Syndrome