Rabbit Cyclin O Polyclonal Antibody | anti-CCNO antibody

Cyclin O Antibody

Cat. Num. AAA150476

• Gene Names: CCNO; CCNU; UDG2; CILD29
• Reactivity: Human, Mouse, Rat
• Applications: ELISA, Western Blot
• Purity: Cyclin O Antibody is affinity chromatography purified via peptide column.
• Synonyms: Cyclin O; Polyclonal Antibody; Cyclin O Antibody; CCNU; UDG2; Cyclin-O; anti-CCNO antibody

• Host: Rabbit
• Reactivity: Human, Mouse, Rat
• Clonality: Polyclonal
• Isotype: IgG
• Specificity: At least two isoforms of Cyclin O are known to exist; this antibody will recognize both isoforms.
• Purity/Purification: Cyclin O Antibody is affinity chromatography purified via peptide column.
• Form/Format: Liquid
• Concentration: 1 mg/mL (varies by lot)
• Sequence Length: 350

• Applicable Applications for anti-CCNO antibody: ELISA (EIA), Western Blot (WB)
• Application Notes: Cyclin O antibody can be used for the detection of Cyclin O by Western blot at 1 - 2 mug/mL.
• Conjugate: Unconjugated
• Immunogen: Cyclin O antibody was raised against a 13 amino acid synthetic peptide from near the center of human Cyclin O.
• Buffer: Cyclin O Antibody is supplied in PBS containing 0.02% sodium azide.
• Preparation and Storage: Cyclin O antibody can be stored at 4 degree C for three months and -20 degree C, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures.

Western Blot (WB)

(Western blot analysis of Cyclin O in human bladder tissue lysate with Cyclin O antibody at (A) 1 and (B) 2 μg/mL.)

Related Product Information for anti-CCNO antibody

Cyclin O Antibody: Cyclin O, also known as CCNO, has recently been identified as a Cdk1- and Cdk2-activating cyclin specific to apoptosis in lymphoid cells. Cyclin O binds to and activates Cdk2 in response to instrinsic apoptotic stimuli such as glucocorticoids or DNA-damaging agents. Supression of Cyclin O expression by shRNA leads to the inhibition of glucocorticoid and DNA-damage-induced apoptosis due to a failure of apical caspase activation while leaving the CD95 death receptor-mediated apoptosis intact. Note: this gene, which had a previous symbol of UNG2, was erroneously identified as a uracil DNA glycosylase. A later publication identified this gene's product as a cyclin protein family member.

NCBI and Uniprot Product Information

• NCBI GI #: 153791755
• NCBI GeneID: 10309
• NCBI Accession #: NP_066970
• NCBI GenBank Nucleotide #: NM_021147.4
• UniProt Accession #: P22674; Q0P6J2; Q9H6B0; Q9UMD5; A8K1W5
• Molecular Weight: 39 kDa
• NCBI Official Full Name: cyclin-O
• NCBI Official Synonym Full Names: cyclin O
• NCBI Official Symbol: CCNO
• NCBI Official Synonym Symbols: CCNU; UDG2; CILD29
• NCBI Protein Information: cyclin-O; cyclin U; cyclin domain containing
• UniProt Protein Name: Cyclin-O
• Protein Family: Cyclin
• UniProt Gene Name: CCNO
• UniProt Entry Name: CCNO_HUMAN

NCBI Description

This gene encodes a member of the cyclin protein family, and the encoded protein is involved in regulation of the cell cycle. Disruption of this gene is associated with primary ciliary dyskinesia-19. Alternative splicing results in multiple transcript variants. This gene, which has a previous symbol of UNG2, was erroneously identified as a uracil DNA glycosylase in PubMed ID: 2001396. A later publication, PubMed ID: 8419333, identified this gene's product as a cyclin protein family member. The UNG2 symbol is also used as a specific protein isoform name for the UNG gene (GeneID 7374), so confusion exists in the scientific literature and in some databases for these two genes. [provided by RefSeq, Jul 2014]

Uniprot Description

CCNO: This gene, which has a previous symbol of UNG2, was erroneously identified as a uracil DNA glycosylase in PubMed ID: 2001396. A later publication, PubMed ID: 8419333, identified this gene's product as a cyclin protein family member. The UNG2 term is also used as a specific protein isoform name for the UNG gene (GeneID 7374), so confusion exists in the scientific literature and in some databases for these two genes.[provided by RefSeq, Nov 2010]

Protein type: DNA repair, damage; Cell cycle regulation; EC 3.2.2.-; Hydrolase

Chromosomal Location of Human Ortholog: 5q11.2

Cellular Component: nucleoplasm; cytoplasm

Molecular Function: uracil DNA N-glycosylase activity

Biological Process: response to drug; cell division; base-excision repair; cilium biogenesis; cell cycle

Disease: Ciliary Dyskinesia, Primary, 29