Mouse anti-Human Thrombospondin Type-1 Domain-containing Protein 1 Monoclonal Antibody | anti-THSD1 antibody

Thrombospondin Type-1 Domain-containing Protein 1 (THSD1, Transmembrane Molecule with Thrombospondin Module, Tmtsp)

Cat. Num. AAA608244

• Gene Names: THSD1; TMTSP; UNQ3010; MGC74971
• Reactivity: Human
• Applications: Flow Cytometry, Functional Assay
• Purity: Affinity Purified; Purified by Protein G affinity chromatography.
• Synonyms: Thrombospondin Type-1 Domain-containing Protein 1; Monoclonal Antibody; Thrombospondin Type-1 Domain-containing Protein 1 (THSD1; Transmembrane Molecule with Thrombospondin Module; Tmtsp); Anti -Thrombospondin Type-1 Domain-containing Protein 1 (THSD1; anti-THSD1 antibody

• Host: Mouse
• Reactivity: Human
• Clonality: Monoclonal
• Isotype: IgG1
• Clone Number: 9j10
• Specificity: Recognizes human THSD1. Species sequence homology: mouse (76%).
• Purity/Purification: Affinity Purified; Purified by Protein G affinity chromatography.
• Form/Format: Supplied as a lyophilized powder from 0.2um filtered solution in PBS, 5% trehalose. Reconstitute with sterile PBS, sterile 40-50% glycerol.

• Applicable Applications for anti-THSD1 antibody: Flow Cytometry (FC/FACS)
• Application Notes: Suitable for use in Flow Cytometry.; Dilution: Flow Cytometry: 25ug/ml
• Immunogen: NS0-derived, recombinant human THSD1 (aa25-361).
• Preparation and Storage: Lyophilized powder may be stored at -20 degree C. Stable for 12 months at -20 degree C. Reconstitute with sterile PBS, sterile 40-50% glycerol, aliquot and store at -20 degree C. Reconstituted product is stable for 12 months at -20 degree C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.

Testing Data

(HUVEC cells were stained with anti-THSD1 (filled histogram) or isotype control (open histogram) followed by APC-conjugated anti-mouse antibody.)

Related Product Information for anti-THSD1 antibody

THSD1 (thrombospondin type 1 domain-containing protein 1; also TMTSP) is a 90-100kD type I transmembrane protein that is reminiscent of Unc5h proteins. It is expressed on embryonic endothelial and hematopoietic stem cells, and may be involved in cell-cell interactions. Mature human THSD1 contains a 388aa extracellular domain (ECD) (aa25-412) and a 418aa cytoplasmic region (aa434-851). The ECD possesses three Ig-like domains (aa156-316) and one TSP type-1 domain (aa340-393). There is one potential alternate start site at Met380, and two splice variants, one of which shows a deletion of aa341-393, and a second that shows a 36aa substitution for aa394-851, generating a soluble form. Over aa25-361, human THSD1 is 76% aa identical to mouse THSD1.

Product Categories/Family for anti-THSD1 antibody

Antibodies; Abs to Thrombospondin

NCBI and Uniprot Product Information

• NCBI GI #: 8923894
• NCBI GeneID: 55901
• NCBI Accession #: NP_061146.1
• NCBI GenBank Nucleotide #: NM_018676.3
• UniProt Accession #: Q9NS62; Q6P3U1; Q6UXZ2; A2A3J3; B2RCF5
• Molecular Weight: 94,584 Da
• NCBI Official Full Name: thrombospondin type-1 domain-containing protein 1 isoform 1
• NCBI Official Synonym Full Names: thrombospondin, type I, domain containing 1
• NCBI Official Symbol: THSD1
• NCBI Official Synonym Symbols: TMTSP; UNQ3010; MGC74971
• NCBI Protein Information: thrombospondin type-1 domain-containing protein 1; 4833423O18Rik; OTTHUMP00000018448; OTTHUMP00000178678; thrombospondin, type I, domain 1; transmembrane molecule with thrombospondin module
• UniProt Protein Name: Thrombospondin type-1 domain-containing protein 1
• Protein Family: Thrombospondin type-1 domain-containing protein
• UniProt Gene Name: THSD1
• UniProt Synonym Gene Names: TMTSP
• UniProt Entry Name: THSD1_HUMAN

NCBI Description

The protein encoded by this gene contains a type 1 thrombospondin domain, which is found in a number of proteins involved in the complement pathway, as well as in extracellular matrix proteins. Alternatively spliced transcript variants encoding different isoforms have been observed for this gene. [provided by RefSeq]

Uniprot Description

THSD1: contains a type 1 thrombospondin domain, which is found in a number of proteins involved in the complement pathway, as well as in extracellular matrix proteins. Alternatively spliced transcript variants encoding different isoforms have been observed for this gene. [provided by RefSeq, Jan 2009]

Protein type: Membrane protein, integral

Chromosomal Location of Human Ortholog: 13q14.3

Cellular Component: cell surface; cytoplasm; extracellular region; integral to membrane

Biological Process: hemopoietic progenitor cell differentiation