Rabbit anti-Human UIMC1 Polyclonal Antibody | anti-UIMC1 antibody

UIMC1 (Ubiquitin Interaction Motif-containing Protein 1, BRCA1-A Complex Subunit RAP80, Receptor-associated Protein 80, RAP80, Retinoid X Receptor-interacting Protein 110, RXRIP110, X2HRIP110)

Cat. Num. AAA648428

• Gene Names: UIMC1; RAP80; X2HRIP110
• Reactivity: Human
• Applications: Western Blot
• Purity: Affinity Purified; Purified by Protein A affinity chromatography.
• Synonyms: UIMC1; Polyclonal Antibody; UIMC1 (Ubiquitin Interaction Motif-containing Protein 1; BRCA1-A Complex Subunit RAP80; Receptor-associated Protein 80; RAP80; Retinoid X Receptor-interacting Protein 110; RXRIP110; X2HRIP110); Anti -UIMC1 (Ubiquitin Interaction Motif-containing Protein 1; anti-UIMC1 antibody

• Host: Rabbit
• Reactivity: Human
• Clonality: Polyclonal
• Isotype: IgG
• Specificity: Recognizes human UIMC1.
• Purity/Purification: Affinity Purified; Purified by Protein A affinity chromatography.
• Form/Format: Supplied as a liquid in PBS, pH 7.2.
• Sequence: MPRRKKKVKEVSESRNLEKKDVETTSSVSVKRKRRLEDAFIVISDSDGEEPKEENGLQKTKTKQSNRAKCLAKRKIAQMTEEEQFALALKMSEQEAREVNSQEEEEEELLRKAIAESLNSCRPSDASATRSRPLATGPSSQSHQEKTTDSGLTEGIWQLVPPSLFKGSHISQGNEAEEREEPWDHTKKTEEEPVSGSSGSWDQSSQPVFENVNVKSFDRCTGHSAEHTQCGKPQESTGRGSAFLKAVQGSGDTSRHCLPTLADAKGLQDTGGTVNYFWGIPFCPDGVDPNQYTKVILCQLEVYQKSLKMAQRQLLNKKGFGEPVLPRPPSLIQNECGQGEQASEKNECISEDMGDEDKEERQESRASDWHSKTKDFQESSIKSLK

• Applicable Applications for anti-UIMC1 antibody: Western Blot (WB)
• Application Notes: Suitable for use in Western Blot.
• Immunogen: Full length human UIMC1, aa1-385 (AAH06078.1).
• Preparation and Storage: May be stored at 4 degree C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20 degree C. Aliquots are stable for at least 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.

Western Blot (WB)

(Western Blot analysis of UIMC1 expression in transfected 293T cell line by UIMC1 polyclonal antibody. Lane 1: UIMC1 transfected lysate (43kD). Lane 2: Non-transfected lysate.)

Product Categories/Family for anti-UIMC1 antibody

Antibodies; Abs to Binding Proteins

NCBI and Uniprot Product Information

• NCBI GI #: 52790440
• NCBI GeneID: 51720
• UniProt Accession #: Q96RL1; Q5XKQ1; Q7Z3W7; Q8N5B9; Q9BZR1; Q9BZR5; Q9UHX7; A8MSA1; B3KMZ1; B4E3N2
• Molecular Weight: 79,727 Da
• NCBI Official Full Name: UIMC1 protein
• NCBI Official Synonym Full Names: ubiquitin interaction motif containing 1
• NCBI Official Symbol: UIMC1
• NCBI Official Synonym Symbols: RAP80; X2HRIP110
• NCBI Protein Information: BRCA1-A complex subunit RAP80; receptor associated protein 80; receptor-associated protein 80; retinoid x receptor interacting protein; retinoid X receptor-interacting protein 110; ubiquitin interaction motif-containing protein 1
• UniProt Protein Name: BRCA1-A complex subunit RAP80
• Protein Family: BRCA1-A complex
• UniProt Gene Name: UIMC1
• UniProt Synonym Gene Names: RAP80; RXRIP110
• UniProt Entry Name: UIMC1_HUMAN

Uniprot Description

Function: Ubiquitin-binding protein that specifically recognizes and binds 'Lys-63'-linked ubiquitin. Plays a central role in the BRCA1-A complex by specifically binding 'Lys-63'-linked ubiquitinated histones H2A and H2AX at DNA lesions sites, leading to target the BRCA1-BARD1 heterodimer to sites of DNA damage at double-strand breaks (DSBs). The BRCA1-A complex also possesses deubiquitinase activity that specifically removes 'Lys-63'-linked ubiquitin on histones H2A and H2AX. Also weakly binds monoubiquitin but with much less affinity than 'Lys-63'-linked ubiquitin. May interact with monoubiquitinated histones H2A and H2B; the relevance of such results is however unclear in vivo. Does not bind Lys-48'-linked ubiquitin. May indirectly act as a transcriptional repressor by inhibiting the interaction of NR6A1 with the corepressor NCOR1. Ref.1 Ref.10 Ref.11 Ref.16 Ref.17 Ref.18 Ref.23 Ref.25 Ref.26

Subunit structure: Interacts with TSP57

By similarity. Component of the BRCA1-A complex, at least composed of the BRCA1, BARD1, UIMC1/RAP80, FAM175A/Abraxas, BRCC3/BRCC36, BRE/BRCC45 and BABAM1/NBA1. In the BRCA1-A complex, interacts directly with FAM175A/Abraxas. Interacts with ESR1, NR6A1 and UBE2I. Ref.1 Ref.9 Ref.11 Ref.12 Ref.13 Ref.14 Ref.17 Ref.18 Ref.21 Ref.22 Ref.23

Subcellular location: Nucleus. Note: Localizes at sites of DNA damage at double-strand breaks (DSBs). Ref.1 Ref.10 Ref.11 Ref.16 Ref.17 Ref.18

Tissue specificity: Expressed in testis, ovary, thymus and heart. Expressed in germ cells of the testis. Ref.1

Domain: The UIM-linker region between the 2 UIM repeats determines the selectivity for 'Lys-63'-linked ubiquitin. The length of the linker is important. The linker reduces the flexibility between the UIM repeats and promotes high-affinity and linkage-selective interactions. Ref.24The Abraxas-interacting region (AIR) mediates the interaction with FAM175A/Abraxas. Ref.24

Post-translational modification: Sumoylated. Ref.9Phosphorylated upon DNA damage by ATM or ATR. Ref.10 Ref.16 Ref.17 Ref.18 Ref.19

Sequence similarities: Belongs to the RAP80 family.Contains 2 UIM (ubiquitin-interacting motif) repeats.

Sequence caution: The sequence AAH06078.1 differs from that shown. Reason: Erroneous termination at position 386. Translated as Glu.