Mouse anti-Human HS3ST4 Monoclonal Antibody | anti-HS3ST4 antibody

HS3ST4 (Heparan Sulfate Glucosamine 3-O-sulfotransferase 4, Heparan Sulfate D-glucosaminyl 3-O-sulfotransferase 4, 3-OST-4, Heparan Sulfate 3-O-sulfotransferase 4, h3-OST-4, 3OST4)

Cat. Num. AAA645632

• Gene Names: HS3ST4; 30ST4; 3OST4; 3-OST-4; h3-OST-4
• Reactivity: Human
• Applications: Immunoprecipitation, Immunohistochemistry
• Purity: Affinity Purified; Purified by Protein A/G affinity chromatography.
• Synonyms: HS3ST4; Monoclonal Antibody; HS3ST4 (Heparan Sulfate Glucosamine 3-O-sulfotransferase 4; Heparan Sulfate D-glucosaminyl 3-O-sulfotransferase 4; 3-OST-4; Heparan Sulfate 3-O-sulfotransferase 4; h3-OST-4; 3OST4); Anti -HS3ST4 (Heparan Sulfate Glucosamine 3-O-sulfotransferase 4; anti-HS3ST4 antibody

• Host: Mouse
• Reactivity: Human
• Clonality: Monoclonal
• Isotype: IgG1
• Clone Number: 12K103
• Specificity: Recognizes human HS3ST4. Does not react to recombinant human HS3ST3B1.
• Purity/Purification: Affinity Purified; Purified by Protein A/G affinity chromatography.
• Form/Format: Supplied as a lyophilized powder in PBS, trehalose. Reconstitute with sterile PBS to 0.5mg/ml.

• Applicable Applications for anti-HS3ST4 antibody: Immunoprecipitation (IP), Immunohistochemistry (IHC)
• Application Notes: Suitable for use in Immunoprecipitation and Immunohistochemistry.; Dilution: Immunohistochemistry: 8-25ug/ml; Immunoprecipitation: 25ug/ml
• Immunogen: Recombinant corresponding to aa184-456 from human HS3ST4 expressed in CHO cell line.
• Preparation and Storage: Lyophilized powder may be stored at -20 degree C. Stable for 12 months at -20 degree C. Reconstitute with sterile PBS. Aliquot to avoid repeated freezing and thawing. Store at -20 degree C. Reconstituted product is stable for 12 months at -20 degree C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.

Immunohistochemistry (IHC)

(Paraffin-embedded sections of human brain (cerebellum) using Mouse Human Heparan Sulfate 3-O- Sulfotransferase 4/HS3ST4 Monoclonal Antibodyat 15ug/ml overnight at 4°C. Before incubation with the primary antibody, tissue was subjected to heat-induced epitope retrieval using Antigen Retrieval Reagent-Basic. Tissue was stained using the Mouse HRP-DAB Cell & Tissue Staining Kit and counter-stained with hematoxylin (blue). Specific staining was localized to the cytoplasm of Purkinje cells. View our protocol for Chromogenic IHC Staining of Paraffin-)

Related Product Information for anti-HS3ST4 antibody

Heparan sulfate is a highly sulfated polysaccharide that can be found on the cell surface and within the extracellular matrix. It is typically covalently attached to the protein core of proteoglycans, such as syndecans and glypicans. Heparin, on the other hand, can be considered as a highly sulfated version of heparan sulfate that is predominantly found in mast cells. Both heparin and heparan sulfate contain disaccharide repeats of uronic acid and N-acetylglucosamine and are modified by the same sulfotransferases. The uronic acid residues can be sulfated at the 2-O position by heparan sulfate 2-O sulfotransferase (HS2ST). The N-acetylglucosamine residues can be sulfated at the N, 3-O, and 6-O positions by N-deacetylase/N-sulfotransferases (NDSTs), heparan sulfate 3-O sulfotransferases (HS3STs) and heparan sulfate 6-O sulfotransferases (HS6STs) respectively. There are seven HS3STs in the human genome. HS3ST4 and HS3ST2 are brain specific and may participate in HS-dependent neurobiologic events. HS3ST4 can generate tetrasulfated heparan sulfate disaccharide, the most highly sulfated sugar found in biological samples, and may have a role in assisting HSV-1 entry and spread. HS3ST4 is a Golgi resident type II membrane protein and has the longest proline rich stem region among all HS3STs.

Product Categories/Family for anti-HS3ST4 antibody

Antibodies; Abs to Enzymes; Sulfotransferase

NCBI and Uniprot Product Information

• NCBI GI #: 104876423
• NCBI GeneID: 9951
• NCBI Accession #: NP_006031.2
• NCBI GenBank Nucleotide #: NM_006040.2
• UniProt Accession #: Q9Y661; Q5QI42; Q8NDC2
• Molecular Weight: 49,799 Da
• NCBI Official Full Name: heparan sulfate glucosamine 3-O-sulfotransferase 4
• NCBI Official Synonym Full Names: heparan sulfate (glucosamine) 3-O-sulfotransferase 4
• NCBI Official Symbol: HS3ST4
• NCBI Official Synonym Symbols: 30ST4; 3OST4; 3-OST-4; h3-OST-4
• NCBI Protein Information: heparan sulfate glucosamine 3-O-sulfotransferase 4; heparan sulfate 3-O-sulfotransferase 4; heparan sulfate 3-O-sulfotransferase-4; heparan sulfate D-glucosaminyl 3-O-sulfotransferase 4
• UniProt Protein Name: Heparan sulfate glucosamine 3-O-sulfotransferase 4
• Protein Family: Heparan sulfate glucosamine 3-O-sulfotransferase
• UniProt Gene Name: HS3ST4
• UniProt Synonym Gene Names: 3OST4; 3-OST-4; Heparan sulfate 3-O-sulfotransferase 4; h3-OST-4
• UniProt Entry Name: HS3S4_HUMAN

NCBI Description

This gene encodes the enzyme heparan sulfate D-glucosaminyl 3-O-sulfotransferase 4. This enzyme generates 3-O-sulfated glucosaminyl residues in heparan sulfate. Cell surface heparan sulfate is used as a receptor by herpes simplex virus type 1 (HSV-1), and expression of this gene is thought to play a role in HSV-1 pathogenesis. [provided by RefSeq, Jul 2008]

Uniprot Description

Function: Sulfotransferase that utilizes 3'-phospho-5'-adenylyl sulfate (PAPS) to catalyze the transfer of a sulfo group to an N-unsubstituted glucosamine linked to a 2-O-sulfo iduronic acid unit on heparan sulfate. Unlike 3-OST-1, does not convert non-anticoagulant heparan sulfate to anticoagulant heparan sulfate

By similarity.

Catalytic activity: 3'-phosphoadenylyl sulfate + [heparan sulfate]-glucosamine = adenosine 3',5'-bisphosphate + [heparan sulfate]-glucosamine 3-sulfate.

Subcellular location: Golgi apparatus membrane; Single-pass type II membrane protein

Probable.

Tissue specificity: Brain-specific. Ref.1

Sequence similarities: Belongs to the sulfotransferase 1 family.