Rabbit anti-Mouse, rat Troponin I (cardiac) Ser150 Polyclonal Antibody | anti-TNNI3 antibody

Anti-Phospho-Ser150 Troponin I (cardiac)

Cat. Num. AAA502216

• Gene Names: Tnni3; Tn1; cTnI
• Reactivity: Mouse, rat
• Applications: Western Blot
• Purity: Affinity Purified (Prepared from rabbit serum by affinity purification via sequential chromatography on phospho- and dephospho-peptide affinity columns.)
• Synonyms: Troponin I (cardiac) Ser150; Polyclonal Antibody; Anti-Phospho-Ser150 Troponin I (cardiac); anti-TNNI3 antibody

• Host: Rabbit
• Reactivity: Mouse, rat
• Clonality: Polyclonal
• Specificity: Specific for the ~25k cardiac troponin I protein phosphorylated at Ser150. Immunolabeling is greatly decreased with lambda-phosphatase treatment.
• Purity/Purification: Affinity Purified (Prepared from rabbit serum by affinity purification via sequential chromatography on phospho- and dephospho-peptide affinity columns.)
• Form/Format: 100 ul in 10 mM HEPES (pH 7.5), 150 mM NaCl, 100 ug per ml BSA and 50% glycerol. Adequate amount of material to conduct 10-mini Western Blots.
• Sequence Length: 211

• Applicable Applications for anti-TNNI3 antibody: Western Blot (WB)
• Application Notes: Quality Control: Western blots performed on each lot.; WB: 1:1000
• Antigen: Phosphopeptide corresponding to amino acid residues surrounding the phospho-Ser150 of mouse troponin I, cardiac (cTnI).
• Immunogen Information: Synthetic phospho-peptide corresponding to amino acid residues surrounding Ser150 conjugated to KLH
• Immunogen Species: Mouse
• Reactivity Assumed Based on 100% Sequence Homology: Human, non-human primate
• Species Reactivity Note: The antibody has been directly tested for reactivity in Western blots with mouse and rat tissue. It is anticipated that the antibody will react with human and non-human primates based on the fact that these species have 100% homology with the amino acid sequence used as antigen.
• Biological Significance: Troponin I (TnI) is 1 of 3 subunits, along with troponin C (TnC) and Troponin T (TnT) of troponin complex found in cardiac (cTnI) and fast skeletal (fsTnI) muscle. cTnI is phosphorylated by protein kinase C and protein kinase A at Ser23/24 (Noland et al, 1995) and is phosphorylated by AMPK at Ser23 and Ser150 (Solis et al, 2011). Evidence suggests that AMPK, a critical regulator of cardiac energetics, prefers phosphorylating Ser150 over Ser23, and may play a role in regulating energy consumption through altering the phosphorylation status of cTnI (Solis et al., 2011).
• Preparation and Storage: Store at -20 degree C; stable for at least 1 year

Testing Data

Related Product Information for anti-TNNI3 antibody

Affinity purified rabbit polyclonal antibody

References

• Raquel Sancho Solis, Ying Ge, Jeffery W. Wlaker. (2011) A Preferred AMPK phosphorylation site adjacent to the inhibitory loop of cardiac and skeletal Troponin I.
• Thomas A. Noland, Jr., Xiaodu Guo, Robert L. Raynor, Nathan M. Jideama, Vera Averyhart-Fullard, R. John Solaro, and J.F. Kuo (1995) Cardiac Troponin I Mutants. J of Biol Chem 270 (43): 25445-25454.

NCBI and Uniprot Product Information

• NCBI GI #: 6678393
• NCBI GeneID: 21954
• NCBI Accession #: NP_033432.1
• NCBI GenBank Nucleotide #: NM_009406.3
• UniProt Accession #: P48787
• Molecular Weight: 25
• NCBI Official Full Name: troponin I, cardiac muscle
• NCBI Official Synonym Full Names: troponin I, cardiac 3
• NCBI Official Symbol: Tnni3
• NCBI Official Synonym Symbols: Tn1; cTnI
• NCBI Protein Information: troponin I, cardiac muscle; cardiac troponin I
• UniProt Protein Name: Troponin I, cardiac muscle
• Protein Family: Troponin
• UniProt Gene Name: Tnni3
• UniProt Entry Name: TNNI3_MOUSE

Uniprot Description

Function: Troponin I is the inhibitory subunit of troponin, the thin filament regulatory complex which confers calcium-sensitivity to striated muscle actomyosin ATPase activity.

Subunit structure: Interacts with TRIM63

By similarity. Binds to actin and tropomyosin. Interacts with STK4/MST1

By similarity.

Post-translational modification: Phosphorylated at Ser-23 and Ser-24 by PRKD1; phosphorylation reduces myofilament calcium sensitivity. Phosphorylated preferentially at Thr-32. Phosphorylation by STK4/MST1 alters its binding affinity to TNNC1 (cardiac Tn-C) and TNNT2 (cardiac Tn-T)

By similarity. Phosphorylated at Ser-43 and Ser-45 by PRKCE; phosphorylation increases myocardium contractile dysfunction. Ref.4 Ref.5

Sequence similarities: Belongs to the troponin I family.