Immunoprecipitation (IP)
(FADD was immunoprecipitated using:Lane A:0.5 mg Hela Whole Cell LysateLane B:0.5 mg Jurkat Whole Cell Lysate0.5 uL anti-FADD rabbit polyclonal antibody and 60 ug of Immunomagnetic beads Protein G.Primary antibody:Anti-FADD rabbit polyclonal antibody,at 1:500 dilution Secondary antibody:Dylight 800-labeled antibody to rabbit IgG (H+L), at 1:5000 dilution Developed using the odssey technique.Performed under reducing conditions.Predicted band size: 23 kDaObserved band size: 23 kDa)
Rabbit anti-Human FADD Polyclonal Antibody | anti-FADD antibody
Anti-FADD Antibody, Rabbit Polyclonal
Reactivity
Human
Applications
Western Blot
Purity
Protein A & Antigen Affinity
Synonyms
FADD; Polyclonal Antibody; Anti-FADD Antibody; Rabbit Polyclonal; FADD Antibody; Rabbit PAb; Antigen Affinity Purified; Anti-GIG3 Antibody; Anti-MORT1 Antibody; Fas (TNFRSF6)-associated via death domain; anti-FADD antibody
Host
Rabbit
Reactivity
Human
Clonality
Polyclonal
Isotype
Rabbit IgG
Specificity
Human FADD
Purity/Purification
Protein A & Antigen Affinity
Form/Format
Liquid. 0.2um filtered solution in PBS
Applicable Applications for anti-FADD antibody
Western Blot (WB)
Application Notes
WB: 1:500-1:1000
Immunogen
A synthetic peptide corresponding to the center region of the Human FADD
Conjugation
Unconjugated
Preparation
Produced in rabbits immunized with a synthetic peptide corresponding to the center region of the Human FADD, and purified by antigen affinity chromatography.
Preparation and Storage
This antibody can be stored at 2-8 degree C for one month without detectable loss of activity. Antibody products are stable for twelve months from date of receipt when stored at -20 degree C to -80 degree C. Preservative-Free. Avoid repeated freeze-thaw cycles.
This antibody is shipped as liquid solution at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.
This antibody is shipped as liquid solution at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.
Immunoprecipitation (IP)
(FADD was immunoprecipitated using:Lane A:0.5 mg Hela Whole Cell LysateLane B:0.5 mg Jurkat Whole Cell Lysate0.5 uL anti-FADD rabbit polyclonal antibody and 60 ug of Immunomagnetic beads Protein G.Primary antibody:Anti-FADD rabbit polyclonal antibody,at 1:500 dilution Secondary antibody:Dylight 800-labeled antibody to rabbit IgG (H+L), at 1:5000 dilution Developed using the odssey technique.Performed under reducing conditions.Predicted band size: 23 kDaObserved band size: 23 kDa)
Immunoprecipitation (IP)
(FADD was immunoprecipitated using:Lane A:0.5 mg Hela Whole Cell LysateLane B:0.5 mg Jurkat Whole Cell Lysate0.5 uL anti-FADD rabbit polyclonal antibody and 60 ug of Immunomagnetic beads Protein G.Primary antibody:Anti-FADD rabbit polyclonal antibody,at 1:500 dilution Secondary antibody:Dylight 800-labeled antibody to rabbit IgG (H+L), at 1:5000 dilution Developed using the odssey technique.Performed under reducing conditions.Predicted band size: 23 kDaObserved band size: 23 kDa)
Western Blot (WB)
(Anti-FADD rabbit polyclonal antibody at 1:500 dilutionLane A: Jurkat Whole Cell LysateLysates/proteins at 30 ug per lane.SecondaryGoat Anti-Rabbit IgG H&L (Dylight800) at 1/10000 dilution.Developed using the Odyssey technique. Performed under reducing conditions.Predicted band size:23 kDaObserved band size:27 kDa(We are unsure as to the identity of these extra bands.))
Western Blot (WB)
(Anti-FADD rabbit polyclonal antibody at 1:500 dilutionLane A: Jurkat Whole Cell LysateLysates/proteins at 30 ug per lane.SecondaryGoat Anti-Rabbit IgG H&L (Dylight800) at 1/10000 dilution.Developed using the Odyssey technique. Performed under reducing conditions.Predicted band size:23 kDaObserved band size:27 kDa(We are unsure as to the identity of these extra bands.))
Related Product Information for anti-FADD antibody
Background: The Fas-Associated Death Domain (FADD) gene is located in the chromosome 11q13-region and frequently is amplified in head and neck squamous cell carcinoma. Expression of FADD and its phosphorylated isoform (pFADD) have been associated with aggressive tumor growth, lymph node metastasis, and overall survival. Previously, we demonstrated that pFADD expression was related to a significantly improved local control in early stage (tumor [T]1 to T2) glottic laryngeal squamous cell carcinoma (LSCC). FADD (Fas-associated death domain) adaptor is a crucial protein involved in the induction of cell death but also mediates non-apoptotic actions via a phosphorylated form (p-Ser194-FADD). Together with the observations of FADD redistribution into tangles and dystrophic neurites within plaques in Alzheimer's disease brains, and its reduction in APP23 mouse cortex, the results suggest this multifunctional protein might participate in the mechanisms linking amyloid and tau pathologies during the course of the illness. Thus FADD as a putative biomarker for pathological processes associated with the course of clinical dementia. The Fas-associated death domain protein (FADD), a classical adaptor protein mediating apoptotic stimuli-induced cell death, protects pancreatic cancer cells from drug-induced apoptosis. In contrast to its classical apoptotic roles, it was observed that FADD is required for pancreatic cancer cell proliferation and that it is overexpressed to varying degrees in various types of pancreatic cancer cell.
Product Categories/Family for anti-FADD antibody
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Product Notes
The FADD (Catalog #AAA8118893) is an Antibody produced from Rabbit and is intended for research purposes only. The product is available for immediate purchase. The Anti-FADD Antibody, Rabbit Polyclonal reacts with Human and may cross-react with other species as described in the data sheet. AAA Biotech's FADD can be used in a range of immunoassay formats including, but not limited to, Western Blot (WB). WB: 1:500-1:1000. Researchers should empirically determine the suitability of the FADD for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "FADD, Polyclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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