Rabbit anti-Rat EphB3 Polyclonal Antibody | anti-EphB3 antibody

Anti-EphB3 Antibody, Rabbit Polyclonal

Cat. Num. AAA8113098

• Reactivity: Rat
• Applications: Western Blot, Immunoprecipitation
• Purity: Protein A & Antigen Affinity
• Synonyms: EphB3; Polyclonal Antibody; Anti-EphB3 Antibody; Rabbit Polyclonal; EphB3/HEK2/Eph Receptor B3 Antibody; Rabbit PAb; Antigen Affinity Purified; EPH receptor B3; anti-EphB3 antibody

• Host: Rabbit
• Reactivity: Rat
• Clonality: Polyclonal
• Isotype: Rabbit IgG
• Specificity: Rat EphB3/HEK2/Eph Receptor B3
• Purity/Purification: Protein A & Antigen Affinity
• Form/Format: Liquid; 0.2um filtered solution in PBS

• Applicable Applications for anti-EphB3 antibody: Western Blot (WB), Immunoprecipitation (IP)
• Application Notes: WB: 1:500-1:1000; IP: 0.2-1uL/mg of lysate
• Immunogen: Recombinant Rat EphB3/HEK2/Eph Receptor B3 Protein
• Conjugation: Unconjugated
• Preparation: Produced in rabbits immunized with purified, recombinant Rat EphB3/HEK2/Eph Receptor B3 (rR EphB3/HEK2/Eph Receptor B3). EphB3/HEK2/Eph Receptor B3 specific IgG was purified by Rat EphB3/HEK2/Eph Receptor B3 affinity chromatography.
• Preparation and Storage: This antibody can be stored at 2-8 degree C for one month without detectable loss of activity. Antibody products are stable for twelve months from date of receipt when stored at -20 degree C to -80 degree C. Preservative-Free. Avoid repeated freeze-thaw cycles.

Immunoprecipitation (IP)

(rat EPHB3 was immunoprecipitated using:Lane A:0.5 mg NIH-3T3 Whole Cell Lysate0.5 uL anti-rat EPHB3 rabbit polyclonal antibody and 60 ug of Immunomagnetic beads Protein G.Primary antibody:Anti-rat EPHB3 rabbit polyclonal antibody,at 1:500 dilution Secondary antibody:Dylight 800-labeled antibody to rabbit IgG (H+L), at 1:5000 dilution Developed using the odssey technique.Performed under reducing conditions.Predicted band size: 110 kDaObserved band size: 110 kDa)

Western Blot (WB)

(Anti-EPHB3 rabbit polyclonal antibody at 1:500 dilution.Lane A: Hela Whole Cell LysateLane B: Mouse heart tissue lysateLysates/proteins at 30 ug per lane.SecondaryGoat Anti-Rabbit IgG H&L (Dylight800) at 1/10000 dilution.Developed using the Odyssey technique. Performed under reducing conditions.Predicted band size:110 kDaObserved band size:110 kDa(We are unsure as to the identity of these extra bands.))

Related Product Information for anti-EphB3 antibody

Ephrin type-B receptor 3, also known as EphB3 or HEK2, belongs to the ephrin receptor subfamily of the protein-tyrosine kinase family which 16 known receptors (14 found in mammals) are involved: EPHA1, EPHA2, EPHA3, EPHA4, EPHA5, EPHA6, EPHA7, EPHA8, EPHA9, EPHA10, EPHB1, EPHB2, EPHB3, EPHB4, EPHB5, EPHB6. The Eph family of receptor tyrosine kinases (comprising EphA and EphB receptors) has been implicated in synapse formation and the regulation of synaptic function and plasticity6. Ephrin receptors are components of cell signalling pathways involved in animal growth and development, forming the largest sub-family of receptor tyrosine kinases (RTKs). Ligand-mediated activation of Ephs induces various important downstream effects and Eph receptors have been studied for their potential roles in the development of cancer. EphB receptor tyrosine kinases are enriched at synapses, suggesting that these receptors play a role in synapse formation or function. We find that EphrinB binding to EphB induces a direct interaction of EphB with NMDA-type glutamate receptors. This interaction occurs at the cell surface and is mediated by the extracellular regions of the two receptors, but does not require the kinase activity of EphB.

Product Categories/Family for anti-EphB3 antibody

Actin Dynamics Signaling Pathway

References

Bergemann AD, et al. (1998) Ephrin-B3, a ligand for the receptor EphB3, expressed at the midline of the developing neural tube. Oncogene. 16(4): 471-80.Hock B, et al. (1998) PDZ-domain-mediated interaction of the Eph-related receptor tyrosine kinase EphB3 and the ras-binding protein AF6 depends on the kinase activity of the receptor. Proc Natl Acad Sci U S A. 95(17): 9779-84.Liu X, et al. (2006) EphB3: an endogenous mediator of adult axonal plasticity and regrowth after CNS injury. J Neurosci. 26(12): 3087-101.

NCBI and Uniprot Product Information

• NCBI GI #: 157787046
• NCBI GeneID: 287989
• NCBI Accession #: NP_001099338.1
• NCBI GenBank Nucleotide #: NM_001105868.1
• UniProt Accession #: D3ZH39
• Molecular Weight: 115,537 Da
• NCBI Official Full Name: ephrin type-B receptor 3
• NCBI Official Synonym Full Names: Eph receptor B3
• NCBI Official Symbol: Ephb3
• NCBI Protein Information: ephrin type-B receptor 3
• UniProt Protein Name: Eph receptor B3 (Predicted)
• Protein Family: Ephrin type-B receptor
• UniProt Gene Name: Ephb3