FADD recombinant protein

FADD recombinant protein

Cat. Num. AAA515129

• Gene Names: FADD; GIG3; MORT1
• Applications: Western Blot
• Synonyms: FADD; FADD recombinant protein; GIG3; MORT1; MGC8528

• Host: E Coli
• Form/Format: 50mM sodium phosphate, pH 7.0, 300mM NaCl, 150mM imidazole, 0.1mM PMSF, 0.25mM DTT, 25% glycerol.
• Sequence Length: 1855

• Applicable Applications for FADD recombinant protein: Western Blot (WB)
• Type: Recombinant Fusion Protein
• Species: Human
• Tag Information: His tag
• Expression System: E.coli
• Source Note: Recombinant full-length human FADD was expressed in E. coli cell
• Preparation and Storage: Store product at -70 degree C. For optimal storage, aliquot target into smaller quantities after centrifugation and store at recommended temperature. For most favorable performance, avoid repeated handling and multiple freeze/thaw cycles.

SDS-PAGE

Related Product Information for FADD recombinant protein

Recombinant full-length human FADD was expressed in E. coli cells using an N-terminal His tag.

Scientific Background: FADD or Fas-Associated protein with Death Domain is an adaptor molecule that mediates death signaling by the Fas-receptor, tumor necrosis factor receptor and TRAIL-receptor. FADD binds to these receptors via the C-terminus Death Domain which then unmasks the N-terminal effector domain of FADD thereby allowing it to recruit caspase-8 and activate the cysteine protease cascade leading to apoptosis (1). Cells lacking FADD are defective in intracellular double-stranded RNA (dsRNA)-activated gene expression, including production of type I (alpha/beta) interferons and are thus very susceptible to viral infection (2).

Product Categories/Family for FADD recombinant protein

Adaptor Proteins; Signaling Proteins - Adaptor Proteins

References

1.Sheikh, M.S et al: The FADD is going nuclear. Cell Cycle, 2004; 2 (4): 346 -7.
2.Balachandran, S. et al : A FADD-dependent innate immune mechanism in mammalian cells. Nature 432: 401-405, 2004.

NCBI and Uniprot Product Information

• NCBI GI #: 215820647
• NCBI GeneID: 8772
• NCBI Accession #: NP_003815.1
• NCBI GenBank Nucleotide #: NM_003824
• UniProt Accession #: Q13158; Q14866; Q6IBR4
• Molecular Weight: ~27 kDa
• NCBI Official Full Name: Homo sapiens Fas (TNFRSF6)-associated via death domain (FADD), mRNA
• NCBI Official Synonym Full Names: Fas (TNFRSF6)-associated via death domain
• NCBI Official Symbol: FADD
• NCBI Official Synonym Symbols: GIG3; MORT1
• NCBI Protein Information: protein FADD; growth-inhibiting gene 3 protein; mediator of receptor induced toxicity; mediator of receptor-induced toxicity; Fas-associating protein with death domain; Fas-associating death domain-containing protein
• UniProt Protein Name: Protein FADD
• Protein Family: Fas-associated death domain protein
• UniProt Gene Name: FADD
• UniProt Synonym Gene Names: MORT1
• UniProt Entry Name: FADD_HUMAN

NCBI Description

The protein encoded by this gene is an adaptor molecule that interacts with various cell surface receptors and mediates cell apoptotic signals. Through its C-terminal death domain, this protein can be recruited by TNFRSF6/Fas-receptor, tumor necrosis factor receptor, TNFRSF25, and TNFSF10/TRAIL-receptor, and thus it participates in the death signaling initiated by these receptors. Interaction of this protein with the receptors unmasks the N-terminal effector domain of this protein, which allows it to recruit caspase-8, and thereby activate the cysteine protease cascade. Knockout studies in mice also suggest the importance of this protein in early T cell development. [provided by RefSeq, Jul 2008]

Uniprot Description

Function: Apoptotic adaptor molecule that recruits caspase-8 or caspase-10 to the activated Fas (CD95) or TNFR-1 receptors. The resulting aggregate called the death-inducing signaling complex (DISC) performs caspase-8 proteolytic activation. Active caspase-8 initiates the subsequent cascade of caspases mediating apoptosis. Involved in interferon-mediated antiviral immune response, playing a role in the positive regulation of interferon signaling. Ref.17 Ref.23 Ref.24 Ref.25

Subunit structure: Can self-associate. Interacts with CFLAR, PEA15 and MBD4. When phosphorylated, part of a complex containing HIPK3 and FAS. May interact with MAVS/IPS1. Interacts with MOCV v-CFLAR protein and LRDD. Interacts (via death domain) with FAS (via death domain). Interacts with CASP8. Ref.10 Ref.11 Ref.12 Ref.13 Ref.14 Ref.17 Ref.23 Ref.24 Ref.25

Tissue specificity: Expressed in a wide variety of tissues, except for peripheral blood mononuclear leukocytes.

Domain: Contains a death domain involved in the binding of the corresponding domain within Fas receptor. Ref.24The interaction between the FAS and FADD death domains is crucial for the formation of the death-inducing signaling complex (DISC). Ref.24

Involvement in disease: Infections, recurrent, associated with encephalopathy, hepatic dysfunction and cardiovascular malformations (IEHDCM) [MIM:613759]: A condition with biological features of autoimmune lymphoproliferative syndrome such as high-circulating CD4(-)CD8(-)TCR-alpha-beta(+) T-cell counts, and elevated IL10 and FASL levels. Affected individuals suffer from recurrent, stereotypical episodes of fever, encephalopathy, and mild liver dysfunction sometimes accompanied by generalized seizures. The episodes can be triggered by varicella zoster virus (VZV), measles mumps rubella (MMR) attenuated vaccine, parainfluenza virus, and Epstein-Barr virus (EBV).Note: The disease is caused by mutations affecting the gene represented in this entry. Ref.17

Sequence similarities: Contains 1 death domain.Contains 1 DED (death effector) domain.