Rabbit anti-Human CPNE1 Polyclonal Antibody | anti-CPNE1 antibody

Anti-CPNE1 Antibody, Rabbit Polyclonal

Cat. Num. AAA8107299

• Gene Names: CPNE1; CPN1; COPN1
• Reactivity: Human
• Applications: Western Blot, ELISA, Immunoprecipitation
• Purity: Protein A & Antigen Affinity
• Synonyms: CPNE1; Polyclonal Antibody; Anti-CPNE1 Antibody; Rabbit Polyclonal; Copine I/CPN1/CPNE1 Antibody; Rabbit PAb; Antigen Affinity Purified; copine I; Anti-COPN1 Antibody; Anti-CPN1 Antibody; anti-CPNE1 antibody

• Host: Rabbit
• Reactivity: Human
• Clonality: Polyclonal
• Isotype: Rabbit IgG
• Specificity: Human Copine I/CPN1/CPNE1
• Purity/Purification: Protein A & Antigen Affinity
• Form/Format: Liquid; 0.2um filtered solution in PBS

• Applicable Applications for anti-CPNE1 antibody: Western Blot (WB), ELISA (EIA), Immunoprecipitation (IP)
• Application Notes: WB: 1:500-1:2000; ELISA: 1:5000-1:10,000; IP: 0.5-2uL/mg of lysate
• Immunogen: Recombinant Human Copine I/CPN1/CPNE1 Protein
• Conjugation: Unconjugated
• Preparation: Produced in rabbits immunized with purified, recombinant Human Copine I/CPN1/CPNE1 (rh Copine I/CPN1/CPNE1). Copine I/CPN1/CPNE1 specific IgG was purified by Human Copine I/CPN1/CPNE1 affinity chromatography.
• Preparation and Storage: This antibody can be stored at 2-8 degree C for one month without detectable loss of activity. Antibody products are stable for twelve months from date of receipt when stored at -20 degree C to -80 degree C. Preservative-Free. Avoid repeated freeze-thaw cycles.

Immunoprecipitation (IP)

(CPNE1 was immunoprecipitated using:Lane A:0.5 mg Hela Whole Cell LysateLane B:0.5 mg HepG2 Whole Cell Lysate2 uL anti-CPNE1 rabbit polyclonal antibody and 60 ug of Immunomagnetic beads Protein G.Primary antibody:Anti-CPNE1 rabbit polyclonal antibody,at 1:100 dilution Secondary antibody:Dylight 800-labeled antibody to rabbit IgG (H+L), at 1:5000 dilution Developed using the odssey technique.Performed under reducing conditions.Predicted band size: 59 kDaObserved band size: 59 kDa)

Western Blot (WB)

(Anti-CPNE1 rabbit polyclonal antibody at 1:500 dilutionLane A: A431 Whole Cell LysateLysates/proteins at 30 ug per lane.SecondaryGoat Anti-Rabbit IgG H&L (Dylight800) at 1/10000 dilution.Developed using the Odyssey technique. Performed under reducing conditions.Predicted band size:59 kDaObserved band size:59 kDa(We are unsure as to the identity of these extra bands.))

Related Product Information for anti-CPNE1 antibody

Copine I, also known as CPN1, is a member of the copine family. Copine I is a calcium-dependent membrane-binding protein which has a wide tissue distribution. Calcium-dependent membrane-binding proteins may regulate molecular events at the interface of the cell membrane and cytoplasm. Copine I contains two N-terminal type II C2 domains and an integrin A domain-like sequence in the C-terminus, while it does not contain a predicted signal sequence or transmembrane domains. Copine I may function in membrane trafficking.

References

Cowland JB, et al. (2003) Tissue expression of copines and isolation of copines I and III from the cytosol of human neutrophils. J Leukoc Biol. 74(3):379-88. Tomsig JL, et al. (2003) Identification of targets for calcium signaling through the copine family of proteins. Characterization of a coiled-coil copine-binding motif. J Biol Chem. 278 (12):10048-54. Strausberg RL, et al. (2003) Generation and initial analysis of more than 15, 000 full-length human and mouse cDNA sequences. Proc Natl Acad Sci. 99(26):16899-903.

NCBI and Uniprot Product Information

• NCBI GI #: 311893345
• NCBI GeneID: 8904
• NCBI Accession #: NP_001185792.1
• NCBI GenBank Nucleotide #: NM_001198863.1
• UniProt Accession #: Q99829; Q6IBL3; Q9H243; Q9NTZ7; E1P5Q4
• Molecular Weight: 59,059 Da
• NCBI Official Full Name: copine-1 isoform c
• NCBI Official Synonym Full Names: copine 1
• NCBI Official Symbol: CPNE1
• NCBI Official Synonym Symbols: CPN1; COPN1
• NCBI Protein Information: copine-1
• UniProt Protein Name: Copine-1
• Protein Family: Copine
• UniProt Gene Name: CPNE1
• UniProt Synonym Gene Names: CPN1

NCBI Description

Calcium-dependent membrane-binding proteins may regulate molecular events at the interface of the cell membrane and cytoplasm. This gene encodes a calcium-dependent protein that also contains two N-terminal type II C2 domains and an integrin A domain-like sequence in the C-terminus. However, the encoded protein does not contain a predicted signal sequence or transmembrane domains. This protein has a broad tissue distribution and it may function in membrane trafficking. This gene and the gene for RNA binding motif protein 12 overlap at map location 20q11.21. Alternate splicing results in multiple transcript variants encoding different proteins. [provided by RefSeq, Aug 2008]

Uniprot Description

Calcium-dependent phospholipid-binding protein that plays a role in calcium-mediated intracellular processes (PubMed:14674885). Involved in the TNF-alpha receptor signaling pathway in a calcium-dependent manner (PubMed:14674885). Exhibits calcium-dependent phospholipid binding properties (PubMed:9430674, PubMed:19539605). Plays a role in neuronal progenitor cell differentiation; induces neurite outgrowth via a AKT-dependent signaling cascade and calcium-independent manner (PubMed:23263657, PubMed:25450385). May recruit target proteins to the cell membrane in a calcium-dependent manner (PubMed:12522145). May function in membrane trafficking (PubMed:9430674). Involved in TNF-alpha-induced NF-kappa-B transcriptional repression by inducing endoprotease processing of the transcription factor NF-kappa-B p65/RELA subunit (PubMed:18212740). Also induces endoprotease processing of NF-kappa-B p50/NFKB1, p52/NFKB2, RELB and REL (PubMed:18212740).