Rabbit anti-Human BID Polyclonal Antibody | anti-BID antibody

Anti-BID Antibody, Rabbit Polyclonal

Cat. Num. AAA8102917

• Gene Names: BID; FP497
• Reactivity: Human
• Applications: Western Blot, ELISA, Immunohistochemistry, Immunoprecipitation
• Purity: Protein A & Antigen Affinity
• Synonyms: BID; Polyclonal Antibody; Anti-BID Antibody; Rabbit Polyclonal; p22 BID Antibody; Rabbit PAb; Antigen Affinity Purified; BH3 interacting domain death agonist; Anti-FP497 Antibody; anti-BID antibody

• Host: Rabbit
• Reactivity: Human
• Clonality: Polyclonal
• Isotype: Rabbit IgG
• Specificity: Human p22 BID
• Purity/Purification: Protein A & Antigen Affinity
• Form/Format: Liquid; 0.2um filtered solution in PBS

• Applicable Applications for anti-BID antibody: Western Blot (WB), ELISA (EIA), Immunohistochemistry-Paraffin (IHC-P), Immunoprecipitation (IP)
• Application Notes: WB: 1:500-1:2000; ELISA: 1:5000-1:10,000; IHC-P: 1:500-1:3000; IP: 0.5-2uL/mg of lysate
• Immunogen: Recombinant Human p22 BID Protein
• Conjugation: Unconjugated
• Preparation: Produced in rabbits immunized with purified, recombinant Human p22 BID (rh p22 BID). p22 BID specific IgG was purified by Human p22 BID affinity chromatography.
• Preparation and Storage: This antibody can be stored at 2-8 degree C for one month without detectable loss of activity. Antibody products are stable for twelve months from date of receipt when stored at -20 degree C to -80 degree C. Preservative-Free. Avoid repeated freeze-thaw cycles.

Immunohistochemistry (IHC)

(Immunochemical staining of human BID in human spleen with rabbit polyclonal antibody at 1:2000 dilution, formalin-fixed paraffin embedded sections.)

Immunohistochemistry (IHC)

(Immunochemical staining of human BID in human placenta with rabbit polyclonal antibody at 1:2000 dilution, formalin-fixed paraffin embedded sections.)

Immunoprecipitation (IP)

(BID was immunoprecipitated using:Lane A:0.5 mg Hela Whole Cell LysateLane B:0.5 mg 293T Whole Cell LysateLane C:0.5 mg RAW264.7 Whole Cell Lysate2 uL anti-BID rabbit polyclonal antibody and 15 ul of 50 % Protein G agarose.Primary antibody:Anti-BID rabbit polyclonal antibody,at 1:200 dilution Secondary antibody:Dylight 800-labeled antibody to rabbit IgG (H+L), at 1:5000 dilution Developed using the odssey technique.Performed under reducing conditions.Predicted band size: 22 kDaObserved band size: 22 kDa)

Western Blot (WB)

(Anti-BID rabbit polyclonal antibody at 1:500 dilutionLane A: A431 Whole Cell LysateLane B: Jurkat Whole Cell LysateLane C: HepG2 Whole Cell LysateLysates/proteins at 30 ug per lane.SecondaryGoat Anti- Rabbit  IgG H&L (Dylight 800)  at 1/10000 dilution.Developed using the Odyssey technique. Performed under reducing conditions.Predicted band size:22 kDaObserved band size:22 kDa(We are unsure as to the identity of these extra bands.))

Related Product Information for anti-BID antibody

The BH3 interacting domain death agonist (BID) is a pro-apoptotic member of the Bcl-2 protein family, which contains only the BH3 domain, and is required for its interaction with the Bcl-2 family proteins and for its pro-death activity. BID is important to cell death mediated by these proteases and thus is the sentinel to protease-mediated death signals. Recent studies further indicate that Bid may be more than just a killer molecule, it could be also involved in the maintenance of genomic stability by engaging at mitosis checkpoint. BID is an integrating key regulator of the intrinsic death pathway that amplifies caspase-dependent and caspase-independent execution of neuronal apoptosis. Therefore pharmacological inhibition of BID provides a promising therapeutic strategy in neurological diseases where programmed cell death is prominent. BID is activated by Caspase 8 in response to Fas/TNF-R1 death receptor activation. Activated BID is translocated to mitochondria and induces cytochrome c release, which in turn activates downstream caspases. BID action has been proposed to involve the mitochondrial re-location of its truncated form, tBid, to facilitate the release of apoptogenic proteins like cytochrome c.

Product Categories/Family for anti-BID antibody

Death Receptor Signaling

References

Gross A. (2006) BID as a double agent in cell life and death. Cell Cycle. 5(6): 582-4.Yin XM. (2007) Bid, a BH3-only multi-functional molecule, is at the cross road of life and death. Gene. 369: 7-19.Esposti MD. (2002) The roles of Bid. Apoptosis. 7(5): 433-40.Yin XM. (2000) Signal transduction mediated by Bid, a pro-death Bcl-2 family proteins, connects the death receptor and mitochondria apoptosis pathways. Cell Res. 10(3): 161-7.Yin XM. (2000) Bid, a critical mediator for apoptosis induced by the activation of Fas/TNF-R1 death receptors in hepatocytes. J Mol Med. 78(4): 203-11.

NCBI and Uniprot Product Information

• NCBI GI #: 4557361
• NCBI GeneID: 637
• NCBI Accession #: NP_001187.1
• NCBI GenBank Nucleotide #: NM_001196.3
• UniProt Accession #: P55957; Q549M7; Q71T04; Q7Z4M9; Q8IY86
• Molecular Weight: 21,995 Da
• NCBI Official Full Name: BH3-interacting domain death agonist isoform 2
• NCBI Official Synonym Full Names: BH3 interacting domain death agonist
• NCBI Official Symbol: BID
• NCBI Official Synonym Symbols: FP497
• NCBI Protein Information: BH3-interacting domain death agonist; p22 BID; BID isoform Si6; BID isoform L(2); BID isoform ES(1b); desmocollin type 4; apoptic death agonist; Human BID coding sequence
• UniProt Protein Name: BH3-interacting domain death agonist
• Protein Family: Bidirectional sugar transporter
• UniProt Gene Name: BID
• UniProt Synonym Gene Names: BID
• UniProt Entry Name: BID_HUMAN

NCBI Description

This gene encodes a death agonist that heterodimerizes with either agonist BAX or antagonist BCL2. The encoded protein is a member of the BCL-2 family of cell death regulators. It is a mediator of mitochondrial damage induced by caspase-8 (CASP8); CASP8 cleaves this encoded protein, and the COOH-terminal part translocates to mitochondria where it triggers cytochrome c release. Multiple alternatively spliced transcript variants have been found, but the full-length nature of some variants has not been defined. [provided by RefSeq, Jul 2008]

Uniprot Description

BID: a pro-apoptotic member of the Bcl-2 superfamily. Targets intracellular membranes and contains a BH3 death domain. Heterodimerizes with either the pro-apoptotic protein BAX or the anti-apoptotic protein BCL2, antagonizing its protective effect. The activity of BID is regulated by Caspase 8-mediated cleavage, exposing the BH3 domain and significantly changing the surface charge and hydrophobicity, causing translocation to mitochondria where it triggers cytochrome c release. Multiple alternatively spliced variants have been found.

Protein type: Apoptosis; Mitochondrial

Chromosomal Location of Human Ortholog: 22q11.1

Cellular Component: mitochondrial outer membrane; membrane; mitochondrion; cytosol

Molecular Function: protein binding; ubiquitin protein ligase binding; death receptor binding

Biological Process: caspase activation; release of cytochrome c from mitochondria; positive regulation of apoptosis; apoptosis; positive regulation of protein homooligomerization; DNA damage response, signal transduction; response to estradiol stimulus; regulation of cell proliferation; positive regulation of protein oligomerization; neuron apoptosis; apoptotic mitochondrial changes; induction of apoptosis via death domain receptors; brain development; protein targeting to mitochondrion; protein homooligomerization